Results 81 to 90 of about 3,074,988 (359)

Simultaneous spatiotemporal super-resolution and multi-parametric fluorescence microscopy

Nature Communications, 2021
Super-resolution microscopy and single molecule fluorescence spectroscopy require optimisation of the temporal or spatial resolution, which are usually mutually exclusive.
Jagadish Sankaran, Harikrushnan Balasubramanian, Wai Hoh Tang, Xue Wen Ng, Adrian Röllin, Thorsten Wohland   +5 more
doaj   +1 more source

Developing Digital Photomicroscopy

Cells, 2022
(1) The need for efficient ways of recording and presenting multicolour immunohistochemistry images in a pioneering laboratory developing new techniques motivated a move away from photography to electronic and ultimately digital photomicroscopy.
Kingsley Micklem
doaj   +1 more source

Super-resolution fluorescence microscopy by stepwise optical saturation [PDF]

Biomed. Opt. Express 9, 1613-1629 (2018), 2018
Super-resolution fluorescence microscopy is an important tool in biomedical research for its ability to discern features smaller than the diffraction limit. However, due to its difficult implementation and high cost, the universal application of super-resolution microscopy is not feasible.
arxiv   +1 more source

The analysis of fluorophore orientation by multiphoton fluorescence microscopy [PDF]

, 2010
The accessibility of tunable, ultrafast laser sources has spurred the development and wide application of specialized microscopy techniques based on chromophore fluorescence following two- and three-photon absorption.
Andrews, D.L., Leeder, J.M.
core   +1 more source

Modifications in FLAP's second cytosolic loop influence 5‐LOX interaction, inhibitor binding, and leukotriene formation

FEBS Letters, EarlyView.
The enzyme 5‐lipoxygenase (5‐LOX) catalyzes the first step in the biosynthesis of leukotrienes (LTs) involved in inflammatory pathophysiology. After cellular stimulation, 5‐LOX translocates to the nucleus, interacting with the 5‐LOX‐activating protein (FLAP) to form LTA4 from arachidonic acid (AA).
Erik Romp, Katharina Rataj, Stefanie König, Marcia E. Newcomer, Oliver Werz, Ulrike Garscha   +5 more
wiley   +1 more source

Calibrating Fluorescence Microscopy With 3D-Speckler (3D Fluorescence Speckle Analyzer)

Bio-Protocol
Fluorescence microscopy has been widely accessible and indispensable in cell biology research. This technique enables researchers to label targets, ranging from individual entities to multiple groups, with fluorescent markers.
Chieh-Chang Lin, Aussie Suzuki
doaj   +1 more source

Fast image reconstruction for fluorescence microscopy

AIP Advances, 2012
Real-time image reconstruction is essential for improving the temporal resolution of fluorescence microscopy. A number of unavoidable processes such as, optical aberration, noise and scattering degrade image quality, thereby making image reconstruction ...
Mahesh Ravi Varma, K. Rajan, Partha Pratim Mondal   +2 more
doaj   +1 more source

Resolution and enhancement in nanoantenna-based fluorescence microscopy

, 2009
Single gold nanoparticles can act as nanoantennas for enhancing the fluorescence of emitters in their near-fields. Here we present experimental and theoretical studies of scanning antenna-based fluorescence microscopy as a function of the diameter of the
Anger P., Buchler B. C., Das P. C., Gleyzes P., Hartschuh A., Höppener C., Höppener C., Kalkbrenner T., Karrai K., Kawata S., Keilmann F., Kreibig U., Kühn S., Kühn S., Kühn S., Lewis A., Mohammadi A., Pfab R. J., Pohl D., Sanchez J., Stoller P., Taflove A., Taminiau T. H., Xie C, Yoskovitz E., Zenhausern F., Zenhausern F.   +26 more
core   +1 more source

Blind fluorescence structured illumination microscopy: A new reconstruction strategy [PDF]

, 2016
In this communication, a fast reconstruction algorithm is proposed for fluorescence \textit{blind} structured illumination microscopy (SIM) under the sample positivity constraint.
Allain, M., Bourguignon, S., Idier, J., Labouesse, S., Liu, P., Negash, A., Sentenac, A.   +6 more
core   +4 more sources

Fourier ring correlation simplifies image restoration in fluorescence microscopy

Nature Communications, 2019
Fourier ring correlation (FRC) has recently gained popularity among fluorescence microscopists as a straightforward and objective method to measure the effective image resolution.
Sami V. Koho, G. Tortarolo, M. Castello, T. Deguchi, A. Diaspro, G. Vicidomini   +5 more
semanticscholar   +1 more source