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PCIB an Antiauxin Enhances Microspore Embryogenesis in Microspore Culture of Brassica juncea

Plant Cell, Tissue and Organ Culture, 2006
An efficient protocol to improve microspore embryogenesis is established in an important oleiferous crop, Brassica juncea (Indian mustard). Colchicine was used for enhancing microspore embryogenesis and also to obtain doubled haploid embryos. Colchicine at high concentrations (>10 mg l−1), for 24 h, proved convenient for direct recovery of diploid ...
Pradeep K Agarwal   +2 more
exaly   +2 more sources

Cereal microspore culture

Plant Science, 1995
The present review will examine some of the recent developments in cereal microspore culture. Experimental procedures as well as applied purposes will be considered. This article will mainly concentrate on the culture of isolated microspores, but due to the close relationship some aspects of anther culture will be discussed also.
Horst Lorz
exaly   +2 more sources

Microspore Embryogenesis in Citrus

2021
This chapter deals with microspore embryogenesis in Citrus. Microspore embryogenesis allows to induce immature gametes (microspores) and to deviate them, in this case, the male one, from the normal gametophytic developmental route in the direction of the sporophytic one, yielding homozygous organisms (embryos and plants).
Yahyaoui E, Germanà Maria
openaire   +2 more sources

Chromatin differentiations in microspores

Experientia, 1971
Peer ...
Esponda, Pedro   +1 more
openaire   +3 more sources

Efficient doubled haploid production in microspore culture of Zengcheng flowering Chinese cabbage (Brassica campestris L. ssp. chinensis [L.] Makino var. utilis Tsen et Lee)

Scientia Horticulturae, 2019
Microspore culture has been implemented in breeding programs to produce doubled haploids (DH) and thus accelerate the breeding process. This study aimed to establish an efficient microspore culture protocol for Zengcheng flowering Chinese cabbage, a ...
Liujing Niu   +3 more
semanticscholar   +1 more source

Barley Isolated Microspore Culture

2021
The production of doubled haploids (DHs) has proved to be a highly valuable tool to obtain new cultivars. Among the cereals, barley (Hordeum vulgare L.) is the most successful species in large-scale haploid production. Techniques employed for this purpose are based on either the gynogenetic or the androgenetic pathway.
Luís, Cistué, Begoña, Echávarri
openaire   +2 more sources

Genetic manipulation of microspores and microspore-derived embryos

In Vitro Cellular & Developmental Biology - Plant, 1992
Recent advances in plant cell and molecular biology have furthered the genetic manipulation of many plant species and advanced the options for crop improvement. Among the many targets for genetic manipulation, microspores offer several unique advantages: they are haploid, single-celled, and highly synchronized. In many plant species microspores develop
openaire   +1 more source

Chemically-induced DNA de-methylation alters the effectiveness of microspore embryogenesis in triticale.

Plant Science, 2019
Microspores exposed to some stress factors may display cell totipotency and could be reprogrammed towards embryogenic development. Plant breeding and genetic engineering widely use haploids/doubled haploids (DHs) derived from in vitro-cultured ...
A. Nowicka   +7 more
semanticscholar   +1 more source

FIRST STAGES OF MICROSPORE REPROGRAMMING TO EMBRYOGENESIS THROUGH ISOLATED MICROSPORE CULTURE IN LOQUAT

Acta Horticulturae, 2011
Peer ...
Padoan D   +6 more
openaire   +3 more sources

Isolated Microspore Culture in Barley

2018
Isolated microspore culture (IMC) is the most efficient way to produce large numbers of doubled-haploid (DH) barley plants in a short time. Yet, while IMC is more cost-efficient and less labor-intensive than anther culture, it is technically more complex and requires more experienced personnel if it is to yield its full potential.
Esteves, Patricio, Belzile, François
openaire   +3 more sources

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