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PCIB an Antiauxin Enhances Microspore Embryogenesis in Microspore Culture of Brassica juncea

Plant Cell, Tissue and Organ Culture, 2006
An efficient protocol to improve microspore embryogenesis is established in an important oleiferous crop, Brassica juncea (Indian mustard). Colchicine was used for enhancing microspore embryogenesis and also to obtain doubled haploid embryos. Colchicine at high concentrations (>10 mg l−1), for 24 h, proved convenient for direct recovery of diploid ...
Agarwal, P.K.   +4 more
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Microspore culture technology

Journal of Tissue Culture Methods, 1989
Methodologies of microspore culture that have been successfully developed in Brassica napus, Zea mays, and Hordeum vulgare, three major, important crop species. However, the protocols can be applied to other plant species effectively, perhaps with some testing and ...
Bin Huang, Wilfred A. Keller
openaire   +1 more source

Isolated Microspore Culture in Barley

2018
Isolated microspore culture (IMC) is the most efficient way to produce large numbers of doubled-haploid (DH) barley plants in a short time. Yet, while IMC is more cost-efficient and less labor-intensive than anther culture, it is technically more complex and requires more experienced personnel if it is to yield its full potential.
Esteves, Patricio, Belzile, François
openaire   +3 more sources

Microspores. Evolution and Ontogeny

Kew Bulletin, 1992
Microsporogenesis - the male programme of development, S.Blackmore and R.B.Knox the phylogenetic context of microsporogenesis, P.R.Crane meiospore formation in charophycean algae, L.E.Graham sporogenesis in bryophytes, R.C.Brown and B.E.Lemmon pteridophyte sporogenesis - a survey of spore wall ontogeny and fine structure in a polyphyletic plant group ...
Simon J. Owens, S. Blackmore, R. B. Knox
openaire   +2 more sources

Isolated wheat microspore culture

Plant Cell, Tissue and Organ Culture, 1995
The use of doubled haploid plants in a wheat breeding program requires an efficient haploid production system. While the techniques for producing doubled haploids from anther culture are well established, those for isolated microspores are complicated and inefficient.
Vicki D. Gustafson   +4 more
openaire   +1 more source

Brassica Improvement Through Microspore Culture

2004
The microspore culture system developed for Brassica species represents one of the most efficient systems available for generating microspore-derived embryos and double haploid plants. Haploid embryos from many Brassica species can be produced at frequencies suitable for both basic research and practical applications.
Ferrie, Alison, Keller, Wilfred
openaire   +2 more sources

Isolated Microspore Culture in Brassica napus

2020
Isolated microspore culture is the most efficient technique among those used to induce microspore embryogenesis. In the particular case of Brassica napus, it is also the most widely used and optimized. In this chapter, we describe a protocol for microspore culture in B.
Patricia, Corral-Martínez   +2 more
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Microspores in Diatoms

Nature, 1939
THE occurrence of microspores in centric diatoms and their possible nature have been the objects of great discussion. Recently, Gross1, on the basis of cultures of Chaetoceros pseudocrinitus and Ch. didymus, concluded that “there is no evidence whatever that these microspore-like bodies found in the cultures of Ch.
openaire   +1 more source

Microspore Culture in Brassica

2003
Pioneering research in Brassica microspore culture (1,2,3)rapidly led to the realization that microspores provide a powerful alternative to protoplast culture as a single-celled culture method in plants. These two single-celled systems are fundamentally different, both in tissue origin and in genetic variability.
openaire   +2 more sources

Single-Stage Carbon Replicas of Microspores

Stain Technology, 1966
Single-stage surface replicas of treated or fresh pollen grains can be made ready for the electron microscope in 1.5 hr. The microspores are discharged into a drop of 50% acetone on a 1 cm square of cleaved mica and air dried. Carbon is evaporated to a film thickness of 35 mμ during rotation of the mica support.
J R, Rowley, J J, Flynn
openaire   +2 more sources

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