Results 11 to 20 of about 12,959 (221)

Effect of the Method of Microspore Isolation on the Efficiency of Isolated Microspore Culture In Vitro for Brassicaceae Family

Horticulturae, 2022
Isolated microspore culture in vitro (IMC) is an advanced technique for producing doubled haploids. We developed a modified microspore isolation method for the Brassicaceae family, which exceeds the results obtained by a standard microspore isolation ...
Elena V. Kozar, Elena G. Kozar, Elena A. Domblides   +2 more
doaj   +2 more sources

Efficient isolated microspore culture protocol for callus induction and plantlet regeneration in japonica rice (Oryza sativa L.)

Plant Methods
Background Isolated microspore culture is a useful biotechnological technique applied in modern plant breeding programs as it can produce doubled haploid (DH) plants and accelerate the development of new varieties.
Runhong Gao, Yingjie Zong, Shuwei Zhang, Guimei Guo, Wenqi Zhang, Zhiwei Chen, Ruiju Lu, Chenghong Liu, Yifei Wang, Yingbo Li   +9 more
doaj   +2 more sources

Microspore Embryogenesis [PDF]

, 2018
Quite separate from this intended pathway of gametogenesis, a microspore can also be induced to assume sporophytic development. “Totipotency”, which is unique to plant cells, allows a microspore that is already destined to develop into a male gametophyte,
T. Silva
semanticscholar   +2 more sources

Early embryo achievement through isolated microspore culture in Citrus clementina Hort. ex Tan., cvs. ‘Monreal Rosso’ and ‘Nules’

Frontiers in Plant Science, 2015
Microspore embryogenesis is a method of achieving complete homozygosity from plants. It is particularly useful for woody species, like Citrus, characterized by long juvenility, a high degree of heterozygosity and often self-incompatibility.
Benedetta eChiancone, Marines eMarli Gniech Karasawa, Valeria eGianguzzi, Ahmed Mohamed Abdelgalel, Ivett eBárány, PILAR S. TESTILLANO, Daniela eTorello Marinoni, Roberto eBotta, Maria Antonietta eGermanà   +8 more
doaj   +2 more sources

Dynamics of Calcium during In vitro Microspore Embryogenesis and In vivo Microspore Development in Brassica napus and Solanum melongena

Frontiers in Plant Science, 2017
Calcium is widely known to have a role as a signaling molecule in many different processes, including stress response and activation of the embryogenic program.
JOSÉ M Segui-Simarro, Antonio Calabuig-Serna, Alba Rivas-Sendra   +2 more
exaly   +2 more sources

Glutathione provides antioxidative defence and promotes microspore-derived embryo development in isolated microspore cultures of triticale (× Triticosecale Wittm.)

Plant Cell Reports, 2018
Depending on the capability for stress adaptation, the role played by glutathione in microspore embryogenesis consists of both antioxidative activity and stimulation of embryo-like structure development. The efficiency of microspore embryogenesis (ME) is
Franciszek Janowiak, Iwona Zur, Kamil Zielinski   +2 more
exaly   +2 more sources

In Vitro Live Cell Imaging Reveals Nuclear Dynamics and Role of the Cytoskeleton During Asymmetric Division of Pollen Mitosis I in Nicotiana Benthamiana. [PDF]

Cytoskeleton (Hoboken)
ABSTRACT Pollen is a male gametophyte of angiosperms. Following meiosis, the microspore undergoes an asymmetric division called pollen mitosis I (PMI), which produces two cells of different sizes: a large vegetative cell and a small generative cell. Polarized nuclear migration and positioning during PMI are important for successful pollen development ...
Mizuta Y, Igarashi M, Shinagawa T, Kaneshiro I, Anan H, Kurihara D.   +5 more
europepmc   +2 more sources

Inhibition of Histone H3K9 Methylation by BIX-01294 Promotes Stress-Induced Microspore Totipotency and Enhances Embryogenesis Initiation

Frontiers in Plant Science, 2017
Microspore embryogenesis is a process of cell reprogramming, totipotency acquisition and embryogenesis initiation, induced in vitro by stress treatments and widely used in plant breeding for rapid production of doubled-haploids, but its regulating ...
Eduardo Berenguer, Ivett Bárány, María-Teresa Solís, Yolanda Pérez-Pérez, María C. Risueño, Pilar S. Testillano   +5 more
doaj   +2 more sources

5-azacytidine promotes microspore embryogenesis initiation by decreasing global DNA methylation, but prevents subsequent embryo development in rapeseed and barley

Frontiers in Plant Science, 2015
Microspores are reprogrammed by stress in vitro towards embryogenesis. This process is an important tool in breeding to obtain double-haploid plants. DNA methylation is a major epigenetic modification that changes in differentiation and proliferation. We
María-Teresa eSolís, Ahmed-Abdalla eEl-Tantawy, Vanesa eCano-Lázaro, María C. Risueño, Pilar S. Testillano   +4 more
doaj   +2 more sources

Chromosome doubling of microspore-derived plants from cabbage (Brassica oleracea var. capitata L.) and broccoli (Brassica oleracea var. italica L.)

Frontiers in Plant Science, 2015
Chromosome doubling of microspore-derived plants is an important factor in the practical application of microspore culture technology because breeding programs require a large number of genetically stable, homozygous doubled haploid plants with a high ...
Suxia eYuan, Yanbin eSu, Yumei eLiu, Zhansheng eLi, Zhiyuan eFang, Limei eYang, Mu eZhuang, Yangyong eZhang, Honghao eLv, Peitian eSun   +9 more
doaj   +2 more sources