Isolation and culture of soybean (Glycine max L. Merrill) microspores and pollen grains [PDF]
In the last three decades, research on soybean microspore embryogenesis was restricted to anther culture, which presents limitations such as the small number of responsive microspores and the high embryogenic potential of sporophytic tissues.
Lia Rosane Rodrigues +2 more
doaj +2 more sources
Microspore Culture in Sunflower (Helianthus Annuus L.) [PDF]
ABSTRACTSeveral parameters of the microspore culture technique in sunflower were tested and a system for isolation of uninucliate, pure and viable microspores was established. The tests are carried out with 8 lines, produced by crosses of wild Helianthus species with H. annuus and F1 generations of 3 hybrids of H. annuus.
M. Drumeva +2 more
openalex +2 more sources
The effect of phytosulfokine alpha on haploid embryogenesis and gene expression of Brassica napus microspore cultures [PDF]
Microspore embryogenesis (ME) is the most powerful tool for creating homozygous lines in plant breeding and molecular biology research. It is still based mainly on the reprogramming of microspores by temperature, osmotic and/or nutrient stress.
Špela Mestinšek Mubi +4 more
doaj +2 more sources
Anther-derived microspore embryogenesis in pepper hybrids orobelle and Bomby [PDF]
Background Traditional breeding methods have long been employed worldwide for the evaluation and development of pepper cultivars. However, these methods necessitate multiple generations of screening, line development, evaluation, recognition, and ...
K. P. Sahana +7 more
doaj +2 more sources
New Prospects for Improving Microspore Embryogenesis Induction in Highly Recalcitrant Winter Wheat Lines [PDF]
Among various methods stimulating biological progress, double haploid (DH) technology, which utilizes the process of microspore embryogenesis (ME), is potentially the most effective.
Ewa Dubas +9 more
doaj +2 more sources
Rapid Introgression of the Fusarium Wilt Resistance Gene into an Elite Cabbage Line through the Combined Application of a Microspore Culture, Genome Background Analysis, and Disease Resistance-Specific Marker Assisted Foreground Selection. [PDF]
Liu X +9 more
europepmc +3 more sources
In vitro androgenesis of triticale in isolated microspore culture [PDF]
Monostori Tamás +3 more
core +2 more sources
Isolated microspore culture in vitro (IMC) is an advanced technique for producing doubled haploids. We developed a modified microspore isolation method for the Brassicaceae family, which exceeds the results obtained by a standard microspore isolation ...
Elena V. Kozar +2 more
doaj +1 more source
Substantial non-homologous recombination and structural variation results from Brassica AABC and CCAB hybrid meiosis. [PDF]
SUMMARY Meiotic crossovers contribute to genetic diversity and play a crucial role in homologous chromosome segregation. Non‐homologous crossovers in Brassica, involving the exchange of genetic material between genomes, can be valuable for transferring novel traits or characteristics between Brassica species.
Lv Z, Mahmoudi S, Mason AS.
europepmc +2 more sources
The Influence of pH on Microspore Embryogenesis of White Cabbage (<i>Brassica oleracea</i> L.)
In vitro microspore culture is one of the top techniques utilised now-a-days for the obtaining of double haploid plants in many plant species, including Brassica. The pH of the medium is a critical factor for the success of In vitro microspore culture as
Tina Oana CRISTEA
doaj +3 more sources

