Results 191 to 200 of about 4,030 (216)
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Colchicine-induced microspore embryogenesis in coffee
Plant Cell, Tissue and Organ Culture, 2002A protocol for the induction of androgenesis and plant regeneration from C. arabica cv. Caturra isolated microspores in vitro using colchicine pretreatment has been developed. Microspores were mechanically isolated and then carefully purified. Before colchicine pretreatment, microspores were cultured in a semi-solid medium for further develop and ...
J.C. Herrera +4 more
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Wheat microspore embryogenesis during in vitro anther culture
Theoretical and Applied Genetics, 1984Cytological analysis of microspore embryogenesis during in vitro culture reveals a high mortality in the first week and a latency phase of about one week before the first embryogénic mitosis. Genotypic differences observed during our wheat anther culture do not seem to originate at the induction level but are linked to the different abortion rates.
Y, Henry +3 more
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Initiation of microspore embryogenesis by stress
Trends in Plant Science, 1997Microspores have the remarkable capacity to develop into haploid plants via embryogenesis in vitro . Stress treatment acts as a trigger for inducing this sporophytic pathway, preventing the development of fertile pollen (gametophytic pathway). The doubled haploids generated are completely homozygous, and represent an important tool for research in ...
Alisher Touraev +2 more
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Microspore Embryogenesis in Almond (Prunus dulcis Mill.)
2018Almond is the most important tree nut crop in terms of commercial production. Its production is limited to areas characterized by a Mediterranean climate, including regions in the Mediterranean countries, the Central Valley of California, Central Asia, the Himalayan slopes and some equivalent areas in the Southern Hemisphere, including Argentina ...
Cimò G, Germanà M
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Scanning electron microscopy of microspore embryogenesis inBrassica spp.
Plant Cell Reports, 1997Scanning electron microscopy was employed to study and compare microspore embryogenesis in vitro with pollen development in planta inBrassica napus andB. oleracea. An exine with its specific pattern had already been formed, when microspores were released from tetrads.
T, Nitta, Y, Takahata, N, Kaizuma
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Proteomics in Rapeseed Microspore Embryogenesis
2008This chapter reviews the proteomics research performed to elucidate the molecular processes associated with embryo initiation and development in Brassica napus (rapeseed) microspore-derived embryo cultures. Classical biochemical methods such as combined [35S]-methionine labelling and 2-D gel electrophoresis (2-DE) have been used for the large-scale ...
J. Cordewener +4 more
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High-frequency embryogenesis inBrassica campestris microspore culture
Plant Cell, Tissue and Organ Culture, 1996Microspore culture is a very important and useful tool in plant breeding for haploid production and has been developed for many years.Brassica campestris (Brassica rapa L. ssp.oleifera) is an important oilseed crop, but it is relatively recalcitrant in tissue culture including microspore culture. The microspore culture in our laboratory is based on the
Yang-Dong Guo, Seppo Pulli
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Programmed Cell Death and Microspore Embryogenesis
2008Programmed cell death (PCD) occurs in the anther during in vivo micro-spore/pollen development. The first hallmarks are detected in the tapetum at meiosis and consist in progressive degeneration of organelles such as mitochondria, as well as DNA degradation into multiple of 180 kpb fragments.
A. L. Varnier, C. Jacquard, C. Clément
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Induction of embryogenesis from isolated apple microspores
Plant Cell Reports, 1999We report, for the first time, the induction of embryogenesis and plant formation from isolated apple (Malus domestica Borkh.) microspores in vitro. Different isolation techniques were tested and an optimized protocol was elaborated. Furthermore, the influence of the induction medium and starvation treatment, using different starvation material ...
M. Höfer, A. Touraev, E. Heberle-Bors
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Effects of brassinosteroids on microspore embryogenesis in Brassica species
In Vitro Cellular & Developmental Biology - Plant, 2005Experiments were conducted to determine the effects of brassinosteroids on microspore embryogenesis in Brassica species. Two compounds, 24-epibrassinolide (EBR) and brassinolide (BL), were evaluated. An increase in embryogenesis was observed in all Brassica napus lines evaluated, including Topas 4079 and several recalcitrant cultivars: Garrison, Westar,
Ferrie, Alison +4 more
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