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Immobilization of enzymes in microtiter plate scale
Biotechnology Journal, 2006AbstractDifferent immobilization methods were adapted to the 96‐well microtiter plate scale using esterases as model enzymes. The methods tested were based on adsorption, coprecipitation, aggregation and covalent bonding. The protein covered microcrystals proved to be the best method in terms of yield and expressed activity for the test reaction, which
Aurelio Hidalgo, Uwe T Bornscheuer
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Biofilm Formation in the 96-Well Microtiter Plate
, 2014 The microtiter plate (also called 96-well plate) assay for studying biofilm formation is a method which allows for the observation of bacterial adherence to an abiotic surface. In this assay, bacteria are incubated in vinyl "U"-bottom or other types of 96-well microtiter plates.
Barbara M, Coffey, Gregory G, Anderson
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A Microtiter Plate Assay for Sulfonamides
Analytical Letters, 1994Abstract Sulfamethoxazole, sulfisoxazole, and sulfadiazine are sulfonamides used in the treatment of several infectious diseases. Several studies have demonstrated that the amino substituent plays an important role in both the toxicity and the therapeutic effect of these drugs.
Timothy J Smith
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Microtiter Plate-Format Optode
Analytical Chemistry, 1998Microtiter plate-format optodes could be assembled by casting bulk-response membranes into the standard 96-well polypropylene-based plate or by screen printing them on an optically transparent substrate with 96-well pattern. The compositions of thick optode membranes, especially the ratios of poly(vinyl chloride) (PVC) to plasticizer [bis-(2-ethylhexyl)
S B, Kim, H C, Cho, G S, Cha, H, Nam
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Synthesis of Sugar Arrays in Microtiter Plate
Journal of the American Chemical Society, 20021,3-Dipolar cycloadditions between azides and alkynes were exploited to attach oligosaccharides to a C(14) hydrocarbon chain that noncovalently binds to the microtiter well surface. Synthesis of sugar arrays was performed on a micromolar scale in situ in the microtiter plate.
Fabio, Fazio +4 more
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A microtiter plate assay for inorganic phosphate
Journal of Biochemical and Biophysical Methods, 1989A microtiter assay for the detection of picomolar quantities of inorganic phosphate has been described. The assay, linear between 50 and 1000 pmol of inorganic phosphate, is simple and rapid, with results obtainable in several minutes. Results from 5'-nucleotidase and (Ca2+ + Mg2+)ATPase assays using this method were compared with conventional ...
M, Hoenig, R J, Lee, D C, Ferguson
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A microtiter plate assay for aspartate transcarbamylase
Analytical Biochemistry, 1990A discontinuous, colorimetric method for the assay of aspartate transcarbamylase has been adapted for use with 96-well microtiter plates. The method is based on that of L.M. Prescott and M.E. Jones (1969 Anal. Biochem. 32, 408-419) for the detection of ureido compounds, using monoxime and antipyrine. The enzymatic reaction is carried out in a volume of
A J, Else, G, Hervé
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Sugar Arrays in Microtiter Plates
2003Presented herein are a variety of methods for the attachment of saccharides to the surface of microtiter plates through long-chain hydrocarbons capable of binding to the polystyrene surface through noncovalent interaction. These glycolipids have the benefit of diverse and facile conjugation procedures and the ability to withstanding a range of ...
Chi-Huey, Wong, Marian C, Bryan
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Parallelized Antibody Selection in Microtiter Plates
2017The most common in vitro technology to generate human antibodies is phage display. This technology is a key technology to select recombinant antibodies for the use as research tools, in diagnostic tests, and for the development of therapeutics.In this review, the high-throughput compatible selection of antibodies (scFv) in microtiter plates is ...
Giulio, Russo +6 more
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