Three-dimensional label-free histological imaging of whole organs by microtomy-assisted autofluorescence tomography [PDF]
iScience, 2022 Summary: Three-dimensional (3D) histology is vitally important to characterize disease-induced tissue heterogeneity at the individual cell level. However, it remains challenging for both high-throughput 3D imaging and volumetric reconstruction.
Yan Zhang +4 more
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Critical Evaluation of Embedding Media for Histological Studies of Early Stages of Chick Embryo Development [PDF]
Methods and Protocols, 2023 A histological examination is an important tool in embryology, developmental biology, and correlated areas. Despite the amount of information available about tissue embedding and different media, there is a lack of information regarding best practices ...
Melyssa Kmecick +4 more
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Ultrasonically Assisted Cutting of Histological Sections for Reducing the Environmental and Financial Impact of Microtomy [PDF]
Chinese Journal of Mechanical Engineering, 2022 Modern-day microtomy requires high precision equipment to thinly section biological tissues. The sectioned tissue must be of good quality not showing cutting tracks or so-called artefacts.
Dong Wang, Daniel De Becker, Anish Roy
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High-Resolution X-Ray Computed Tomography: A New Workflow for the Analysis of Xylogenesis and Intra-Seasonal Wood Biomass Production [PDF]
Frontiers in Plant Science, 2021 Understanding tree growth and carbon sequestration are of crucial interest to forecast the feedback of forests to climate change. To have a global understanding of the wood formation, it is necessary to develop new methodologies for xylogenesis ...
Romain Lehnebach +7 more
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Histological Processing of Scaffolds: Challenges and Solutions [PDF]
Journal of Functional BiomaterialsScaffolds are widely used in bioengineering, both as 3D native tissue-mimicking models for investigating mechanisms under physiological and pathological conditions and also as implantable agents in regenerative medicine.
Tomas Ragauskas +2 more
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Preparation of Zeolites for TEM Using Microtomy [PDF]
MRS Proceedings, 1987 ABSTRACTThe application of microtomy to Transmission Electron Microscopy (TEM) specimen preparation of zeolite catalysts is explained. Using a new acrylic resin (LR White®) thin sections (≤60 nm) may be cut with relative ease. Although the details described are specific to catalysts, microtomy and the use of the acrylic resin are applicable to any hard
R. Csencsits, R. Gronsky
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Microtomy of Large Particle Zeolites for Tem [PDF]
MRS Proceedings, 1990 ABSTRACTUniformly thin specimens of powders, suitable for electron microscopy, can be prepared by ultramicrotomy. There is a size limit to the particles that can be studied since larger particles tend to be dislodged from the available resins when microtomed. A method is described to strengthen the binding of the organic resin to the inorganic zeolite,
J. G. Ulan +2 more
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Label-free automated three-dimensional imaging of whole organs by microtomy-assisted photoacoustic microscopy [PDF]
Nature Communications, 2017 The state-of-the-art three-dimensional biomedical imaging often requires specific tissue preparation that may alter the physical properties of the specimen causing loss of information. Here Wong et al.
Terence T. W. Wong +10 more
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Standardization of DNA extraction from paraffinized spleen samples: molecular diagnosis of human malaria [PDF]
Malaria Journal, 2023 Background Plasmodium vivax is the main species responsible for human malaria in Brazil, and one of its manifestations is splenic malaria, though there are still challenges in its diagnosis. The present study aimed to standardize Plasmodium sp.
Raimunda Sandra Pacheco Souza +7 more
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Optik & Photonik, 2007 AbstractThe laser microtome (LMT) has been designed to slice biological tissue and various materials with high precision. The cutting process is performed by ultra short laser pulses in the near infrared region, inducing an optical breakdown below the surface of the material. Biological Tissue can be processed in its native state without prior fixation
Holger Lubatschowski
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