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Critical Evaluation of Embedding Media for Histological Studies of Early Stages of Chick Embryo Development [PDF]
Methods and Protocols, 2023 A histological examination is an important tool in embryology, developmental biology, and correlated areas. Despite the amount of information available about tissue embedding and different media, there is a lack of information regarding best practices ...
Melyssa Kmecick +4 more
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Histological Processing of Scaffolds: Challenges and Solutions [PDF]
Journal of Functional BiomaterialsScaffolds are widely used in bioengineering, both as 3D native tissue-mimicking models for investigating mechanisms under physiological and pathological conditions and also as implantable agents in regenerative medicine.
Tomas Ragauskas +2 more
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High-Resolution X-Ray Computed Tomography: A New Workflow for the Analysis of Xylogenesis and Intra-Seasonal Wood Biomass Production [PDF]
Frontiers in Plant Science, 2021 Understanding tree growth and carbon sequestration are of crucial interest to forecast the feedback of forests to climate change. To have a global understanding of the wood formation, it is necessary to develop new methodologies for xylogenesis ...
Romain Lehnebach +7 more
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Limits of Vibrating Microtomy [PDF]
Journal of Histochemistry & CytochemistryThe Vibratome, or vibrating microtome allows sectioning of the mouse brain with a reliably established thickness from 50 µm, but they have a strong limitation on the size of the sample suitable for cutting. Herein is described the construction with publicly available element base (from parts for a 3D printer) a so-called 3D-vibrating microtome capable ...
Klepukov A.
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Mastering the art of sectioning: a comprehensive guide to slide-microtome technology and histological applications [PDF]
Frontiers in Veterinary ScienceThe well-known technique of microtomy, which is an essential cutting tool, was first developed for light and transmission electron microscope uses, but it is currently also utilized to prepare specimens for atomic force microscopy (AFM), ion microscopy ...
Yasmin M. Abd El-Aziz +9 more
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Standardization of DNA extraction from paraffinized spleen samples: molecular diagnosis of human malaria [PDF]
Malaria Journal, 2023 Background Plasmodium vivax is the main species responsible for human malaria in Brazil, and one of its manifestations is splenic malaria, though there are still challenges in its diagnosis. The present study aimed to standardize Plasmodium sp.
Raimunda Sandra Pacheco Souza +7 more
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Colorectal cancer biofilm composition reveals distinct bacterial species signature [PDF]
Applied Microbiology and BiotechnologyHuman colon hosts a highly organized protective microbial ecosystem in the form of biofilms, increasingly recognized as key contributors to colorectal cancer (CRC) progression through microbial dysbiosis and complex host-microbiota interactions. In India,
Manish Kushwaha +6 more
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Taxonomic relevance of petiole anatomical and micro-morphological characteristics of Clematis L. (Ranunculaceae) taxa from South Korea [PDF]
PeerJ, 2021 We assessed the micro-morphological and anatomical structures of the petioles of 19 Clematis taxa from South Korea. The petiole surface features were observed with the help of stereomicroscopy and scanning electron microscopy, and the anatomical features
Beom Kyun Park +2 more
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iScience, 2022 Summary: Three-dimensional (3D) histology is vitally important to characterize disease-induced tissue heterogeneity at the individual cell level. However, it remains challenging for both high-throughput 3D imaging and volumetric reconstruction.
Yan Zhang +4 more
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Chinese Journal of Mechanical Engineering, 2022 Modern-day microtomy requires high precision equipment to thinly section biological tissues. The sectioned tissue must be of good quality not showing cutting tracks or so-called artefacts.
Dong Wang, Daniel De Becker, Anish Roy
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