Results 71 to 80 of about 271 (212)

Review of Triply Periodic Minimal Surface (TPMS) Structures for Cooling Heat Sinks

Energies
This review paper deals with Triply Periodic Minimal Surfaces (TPMS) and lattice structures as a new generation of heat exchangers. Especially, their manufacturing is becoming feasible with technological progress.
Khaoula Amara, Mohamad Ziad Saghir, Ridha Abdeljabar   +2 more
doaj   +1 more source

A new flow chip in combination with multiphoton microscopy as a protocol for longitudinal 3D imaging of tissue calcification under shear stress

FEBS Open Bio, EarlyView.
Miniaturized flow chip platform enabling continuous perfusion and longitudinal multiphoton 3D imaging of vascular smooth muscle cell constructs under physiological flow. Brightfield imaging guides region selection, while CellTracker Green and mRuby‐labeled fetuin‐A visualize cells and mineral deposition, respectively. Magnesium supplementation markedly
Vytautas Kučikas, Aaron D. Morgan, Ignas Čiurlionis, Christian Böhm, Stefan Jockenhövel, Willi Jahnen‐Dechent, Marc A. M. J. van Zandvoort   +6 more
wiley   +1 more source

Optimizing photoexcitation conditions for time‐resolved X‐ray solution scattering experiments

FEBS Open Bio, EarlyView.
Time‐resolved X‐ray solution scattering (TR‐XSS) is a powerful technique to visualize how proteins change their structure in real time after light activation. Selecting the right laser photoexcitation conditions—fluence, excitation geometry, and sample refresh rate—is critical to maximize the experimental signal while avoiding unwanted side effects ...
Matteo Levantino
wiley   +1 more source

Delivery of DNA‐encoded vaccines and proteins in mice using cutaneous suction‐mediated transfection

FEBS Open Bio, EarlyView.
Cutaneous suction‐mediated transfection in mice for delivery of DNA‐encoded vaccines and proteins. A critical limitation of DNA vaccines and other therapeutics is transfection in vivo to produce the encoded antigens or therapeutic proteins. Cutaneous suction‐based methods have demonstrated effectiveness in many animal models and have been successfully ...
Emran O. Lallow, Isabel Brandtjen, Yaxin Mo, Melissa Gulley, Louis Osorio, Sagar Kudchodkar, Nandita C. Jhumur, Christine C. Roberts, Lisa K. Denzin, David I. Shreiber, Biju Parekkadan, Hao Lin, Joel N. Maslow   +12 more
wiley   +1 more source

Structural studies and functional engineering of NanX: an anhydro‐sialic acid transporter from Escherichia coli

FEBS Open Bio, EarlyView.
Biophysical characterisation shows that NanX, a membrane transport protein from the major facilitator superfamily (MFS), forms both monomers and dimers after purification. AlphaFold modelling and substrate docking provide information on residues likely involved in substrate recognition for NanX and another MFS member, NanT.
Michael C. Newton‐Vesty, Kelsi R. Hall, Zachary D. Tillett, Liam S. Turk, James S. Davies, Michael J. Currie, Hamish G. Brown, Sepideh Valimehr, Peter D. Mace, Andrew E. Whitten, Santosh Panjikar, Eric Hanssen, Rachel A. North, Renwick C.J. Dobson   +13 more
wiley   +1 more source

Revealing the mechanism of crystal orientation-dependent surface morphology and topography evolution in single-crystal ZnO using nanoindentation

Materials & Design
Single-crystal zinc oxide (ZnO) is an important optoelectronic material, but its micro/nanoscale mechanical behavior is poorly understood. In this study, nanoindentation tests were performed on various crystalline planes of ZnO crystals using a Berkovich
Weihai Huang, Jiwang Yan
doaj   +1 more source

Purification and preparation of Marchantia polymorpha Auxin Response Factor 2 for phase separation studies

FEBS Open Bio, EarlyView.
We describe detailed protocols for the purification and preparation of Marchantia polymorpha Auxin Response Factor 2 (MpARF2). This protein is fused to an MBP solubility tag and an mNG fluorescent tag and is purified from Escherichia coli. The presented procedures make it possible to study MpARF2 assemblies, which could arise from phase separation ...
Bas Janssen, Robin Romein, Willy A. M. van den Berg, Simon Lindhoud, Jan Willem Borst, Carlo P. M. van Mierlo   +5 more
wiley   +1 more source

The influence of different surface texture parameters on the cell proliferation and adhesion early behaviors on the surface of titanium alloy

Frontiers in Bioengineering and Biotechnology
Current clinical practice has extensively validated the efficacy of left ventricular assist devices (LVADs) in managing end-stage heart failure. A persistent challenge across all ventricular assist systems involves achieving optimal biocompatibility at ...
Fuqiang Lai, Fuqiang Lai, Fuqiang Lai, Changsheng Cao, Linfeng Xie, Linfeng Xie, Mingxuan Shang, Wenge Liu, Yumei Li, Yumei Li, Zhihuang Qiu, Zhihuang Qiu, Liangwan Chen, Liangwan Chen   +13 more
doaj   +1 more source

Protocol for quantifying miRNA trafficking across the endosomal membrane

FEBS Open Bio, EarlyView.
An in vitro protocol measures miRNA uptake into endosomes isolated from mammalian cell extracts, which are free of subcellular contaminants. Performed at 37 °C in the presence of ATP, it ensures the import of single‐stranded miRNA into the endosomal lumen.
Syamantak Ghosh, Kamalika Mukherjee, Suvendra N. Bhattacharyya   +2 more
wiley   +1 more source

Comparative Study on EDM Wire Cutting and CO2 Laser Cutting for High‐Precision Stainless Steel Sheet Processing

Engineering Reports
CO2 laser cutting and electrical discharge machining (EDM) wire cutting are two advanced thermal energy‐based methods widely utilized for precision profile cutting tasks.
A. Parthiban, K. Ananthakumar, S. Ajith Arul Daniel, S. Sivaganesan, T. Sathish, Jayant Giri, A. Johnson Santhosh, Ahmad O. Hourani   +7 more
doaj   +1 more source