Results 121 to 130 of about 3,372 (157)

Minisatellite variant repeat (MVR) mapping: analysis of ‘null’ repeat units at D1S8

Human Molecular Genetics, 1992
Minisatellite variant repeat mapping by PCR (MVR-PCR) is a new approach to studying variation in human DNA which analyses interspersion patterns of variant repeats within minisatellite arrays. MVR-PCR has been applied to the hypervariable human minisatellite D1S8 which contains two major classes of variant 29bp repeat units designated a-type and t-type.
K, Tamaki, D G, Monckton, A, MacLeod, D L, Neil, M, Allen, A J, Jeffreys   +5 more
openaire   +3 more sources

Minisatellite variant repeat (MVR) analysis of the HRAS1 minisatellite locus

ELECTROPHORESIS, 1998
AbstractTwo alternative electrophoretic strategies were used to study the internal variation of the HRAS1 minisatellite after minisatellite variant repeat mapping (MVR‐PCR) was carried out. While the use of automated sequencers with fluorescent based technology is ideal for analyzing fragment size, and therefore, for analyzing the repeat number, the ...
A, Vega, F, Barros, S, Bellas, A, Carracedo   +3 more
openaire   +2 more sources

Minisatellite repeat coding as a digital approach to DNA typing

Nature, 1991
Most DNA typing systems used in forensic and legal medicine assay allelic length variation at tandem repetitive DNA regions such as minisatellites. A simple alternative approach that displays patterns of variant repeat units along minisatellite alleles is described here.
Jeffreys, AJ, MacLeod, A, Tamaki, K, Neil, DL, Monckton, DG   +4 more
openaire   +3 more sources

PCR‐free digital minisatellite tandem repeat genotyping

ELECTROPHORESIS, 2011
AbstractWe demonstrated a proof‐of‐concept for novel minisatellite tandem repeat typing, called PCR‐free digital VNTR (variable number tandem repeat) typing, which is composed of three steps: a ligation reaction instead of PCR thermal cycling, magnetic bead‐based solid‐phase capture for purification, and an elongated sample stacking microcapillary ...
Chen, Y Chen, Yuchao, Seo, TS SEO, TAE SEOK   +1 more
openaire   +2 more sources

Human minisatellites, repeat DNA instability and meiotic recombination

Electrophoresis, 1999
Minisatellites include some of the most variable loci in the human genome and are superb for dissecting processes of tandem repeat DNA instability. Single DNA molecule analysis has revealed different mutation processes operating in the soma and germline. Low-level somatic instability results in simple intra-allelic rearrangements.
Jeffreys, AJ, Barber, R, Bois, P, Buard, J, Dubrova, YE, Grant, G, Hollies, CR, May, CA, Neumann, R, Panayi, M, Ritchie, AE, Shone, AC, Signer, E, Stead, JD, Tamaki, K   +14 more
openaire   +2 more sources

Distribution of tandem repeat polymorphism within minisatellite MS621 (D5S110)

Annals of Human Genetics, 1996
SummaryThe minisatellite MS621 (D5S110) is a highly polymorphic tandem repeat locus which maps to the distal region of human chromosome 5p. Major repeat unit variants at D5S110 differ not by base substitutions but by differences in the repetition of an 11 bp sequence motif found within each repeat. The two major classes of repeat unit thus contain two (
Armour, JAL, Crosier, M, Jeffreys, AJ
openaire   +3 more sources

Differences and similarities between various tandem repeat sequences: Minisatellites and microsatellites

Biochimie, 1997
Tandemly repetitive DNA sequences are abundantly interspersed in the genome of practically all eukaryotic species studied. The relative occurrence of one type of repetitive sequence and its location in the genome appear to be species specific. A common property of repetitive sequences within the living world is their ability to give rise to variants ...
H, Debrauwere, C G, Gendrel, S, Lechat, M, Dutreix   +3 more
openaire   +2 more sources

Characterization of a minisatellite repeat locus in the chloroplast genome of Orchis palustris (Orchidaceae)

Current Genetics, 2001
We describe the occurrence of a tandem repeat in the chloroplast genome of the marsh orchid, Orchis palustris. The repeat unit is an AT-rich, 16-bp sequence located in the chloroplast tRNALEU intron. Southern blot analysis confirmed that the O. palustris tRNALEU intron including the minisatellite locus has not been transferred to the nucleus, but is ...
CAFASSO D., PELLEGRINO G., WIDMER A., MUSACCHIO A., COZZOLINO, SALVATORE   +4 more
openaire   +5 more sources

Human minisatellite loci composed of interspersed GGA-GGT triplet repeats

Proceedings of the Royal Society of London. Series B: Biological Sciences, 1995
We have isolated two tandemly repeated loci from human DNA which contain long blocks of GGA and GGT trinucleotide repeats. These two repeat unit types, together with other less common variants, are apparently irregularly interspersed along each repeat array.
Armour, JA, Crosier, M, Malcolm, S, Chan, JC, Jeffreys, AJ   +4 more
openaire   +3 more sources

Minisatellite-like repeat sequences in the genome of rodent malaria parasites.

Acta Leidensia, 1992
Using minisatellite DNA probes and various Southern blots containing DNA samples from rodent malaria parasites it was shown that minisatellite-like sequences occur in the genome of these Plasmodium species. In contrast to the high copy number as observed in higher eukaryotes, the use of fingerprinting techniques on DNA from these parasites reveals that
A, van Belkum, G, Trommelen, A, Uitterlinden   +2 more
openaire   +2 more sources