Primer ID MiSeq Library Prep v1
, 2017 This is the protocol to prepare Primer ID MiSeq sequencing library. Viral RNA was first extracted using QIAamp viral RNA extraction kit. The block of random nucleotides (Ns) in the cDNA primers served as the Primer ID. The Superscript III kit was used for the cDNA synthesis. We used two rounds of PCR to amplify the cDNA and incorporate Illumina indexed
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Improved COI taxonomic assignment workflow enhances detection of invertebrates in environmental DNA
Limnology and Oceanography: Methods, EarlyView.Abstract Environmental DNA (eDNA) metabarcoding is an increasingly popular and powerful tool in ecology, offering a non‐invasive method to assess biodiversity and monitor ecosystem health across diverse environments. While the mitochondrial cytochrome c oxidase subunit I (COI) gene is one of the most widely used markers for studying eukaryotic ...
Yuanyu Cheng +5 more
wiley +1 more source
A MiSeq-HyDRA platform for enhanced HIV drug resistance genotyping and surveillance
Scientific Reports, 2019 Conventional HIV drug resistance (HIVDR) genotyping utilizes Sanger sequencing (SS) methods, which are limited by low data throughput and the inability of detecting low abundant drug resistant variants (LADRVs).
Tracy Taylor +10 more
doaj +1 more source
Molecular epidemiology of waterborne zoonoses in the North Island of New Zealand : a thesis presented in partial fulfilment of the requirements for the degree of Doctor of Philosophy in Veterinary Science (Epidemiology and Public Health) at Institute of Veterinary, Animal and Biomedical Sciences (IVABS), Massey University, Palmerston North, New Zealand [PDF]
, 2016 Campylobacter, Cryptosporidium and Giardia species are three important waterborne zoonotic pathogens of global public health concern. This PhD opens with an interpretive overview of the literature on Campylobacter, Cryptosporidium and Giardia spp.
Shrestha, Rima Devi
core
Sediment trap preservative choice impacts particle‐associated microbial community composition
Limnology and Oceanography: Methods, EarlyView.Abstract Understanding and quantifying the microbial contribution to particulate organic matter (POM) flux and carbon cycling in the ocean relies largely on the collection of sinking POM. A critical aspect of evaluating microbial communities on sediment trap‐collected POM is their preservation until recovery and sample processing.
Nicola L. Paul +7 more
wiley +1 more source
Modulation of microRNA editing, expression and processing by ADAR2 deaminase in glioblastoma. [PDF]
, 2015 Background: ADAR enzymes convert adenosines to inosines within double-stranded RNAs, including microRNA (miRNA) precursors, with important consequences on miRNA retargeting and expression.
Alon, S +10 more
core +2 more sources
A case of yogurt central line‐associated bloodstream infection in a child with intestinal failure
Nutrition in Clinical Practice, EarlyView.Abstract Central line‐associated bloodstream infections (CLABSI) are a significant cause of morbidity in children with intestinal failure (IF). Probiotic therapy is discouraged in patients with IF because of the increased risk of bacteremia with the probiotic organism.
Nasiha Rahim +6 more
wiley +1 more source
Draft Genome Sequence of Campylobacter jejuni 11168H [PDF]
, 2017 Campylobacter jejuni is the most prevalent cause of food-borne gastroenteritis in the developed world. The reference and original sequenced strain C. jejuni NCTC11168 has low levels of motility compared to clinical isolates.
Boetzer +13 more
core +4 more sources
GenomeTrakr WGS Protocol Collection and Workflow for MiSeq v1
, 2022 Here we have created a collection of all the protocols used for WGS using the MiSeq, in order, from sample extraction to NCBI submission. This collection has three sections: Section 1: WGS Wet lab workflow for Illumina MiSeq Section 2: Dry lab workflow for sequence QC and NCBI submission - Direct Submission Section 3: Dry lab workflow for sequence ...
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Clinical metagenomic sequencing - CSF RNA and DNA Illumina MiSeq v1
, 2022 This protocol was developed to evaluate unbiased cerebrospinal fluid metagenomics at a referral centre in South Africa. Commercial kit protocols are generally used unmodified and limited use of in-house developed methods or reagents is made. This maintains the simplicity and accessibility of the protocol which can serve as a base for evaluation of ...
Gert Marais +8 more
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