Results 121 to 130 of about 120,736 (335)

Building a custom large-scale panel of novel microhaplotypes for forensic identification using MiSeq and Ion S5 massively parallel sequencing systems.

Forensic Science International: Genetics, 2019
A large number of new microhaplotype loci were identified in the human genome by applying a directed search with selection criteria emphasizing short haplotype length (
M. Puente, M. Puente, Christopher Phillips, C. Xavier, J. Amigo, A. Carracedo, W. Parson, W. Parson, M. Lareu   +8 more
semanticscholar   +1 more source

Cost per sample with varying batching schemes for MiSeq and iSeq platforms.

, 2019
WGS depth of coverage calculated assuming M. tuberculosis genome and 2 × 150 bp runs. Minimum of 30× coverage was used for all scenarios. The bar graph depicts the batching size for the different scenarios. Red, brown, green, blue, and purple data depict
Rebecca E. Colman (254707), Aurélien Mace (6650654), Haifa Mshaiel (6650660), Claudia M. Denkinger (501657), David M. Engelthaler (117020), Darrin Lemmer (773307), Timothy C. Rodwell (175176), Anita Suresh (23218), Jonathan Hetzel (6650657), Donald G. Catanzaro (175160), Amanda G. Young (6650663), Marva Seifert (709453)   +11 more
core   +1 more source

Dietary Baicalin Supplementation Can Enhance the Growth Performance of Weaned Piglets and Maintain the Intestinal Barrier Integrity

Animal Research and One Health, EarlyView.
This research innovatively illustrates baicalin’s multifaceted mechanisms in enhancing piglets’ intestinal health: modulating bile acid metabolism via probiotics, reinforcing tight junction proteins (ZO‐1/claudin), suppressing TLR4/NF‐κB‐mediated inflammation, whereas promoting growth and reducing diarrhea.
Yuhui Gao, Jie Liu, Rui Yang, Xizhen Fan, Cheng Liang, Xin'e Shi, Jianjun Jin   +6 more
wiley   +1 more source

Primer ID MiSeq Library Prep v3 [PDF]

, 2018
This is the protocol to prepare Primer ID MiSeq sequencing library. Viral RNA was first extracted using QIAamp viral RNA extraction kit. The block of random nucleotides (Ns) in the cDNA primers served as the Primer ID. The Superscript III kit was used for the cDNA synthesis. We used two rounds of PCR to amplify the cDNA and incorporate Illumina indexed
openaire   +1 more source

Effects of Lycium barbarum Residue Substituting Dietary Maize on Fecal Microbiota and Growth Performance of Crossbred Simmental Cattle

Animal Research and One Health, EarlyView.
The bioactive substances we extracted from Lycium barbarum residue (LBR) have a typical polysaccharide structure. When 1.80% LBR was used to replace maize in the diet, the average daily gain of rossbred Simmental was significantly increased, and anti‐inflammatory ability was improved. In addition, LBR improved the fecal microbial composition, increased
Kun Cai, Mingtan Li, Lihang He, Ning Ma, Haoyu Liu, Chi Xu, Xianhua Wu, Zeguang Xing, Qian Xing, Yuankai Qu, Xi Ma   +10 more
wiley   +1 more source

Targeted fetal cell‐free DNA screening for aneuploidies in 4,594 pregnancies: Single center study

Molecular Genetics & Genomic Medicine, 2019
Background Next‐generation sequencing (NGS) and discovery of fetal cell‐free DNA (cfDNA) in the maternal circulation render possible prenatal screening for trisomy 21 (Down syndrome), trisomy 18, trisomy 13, and sex chromosome aneuploidies.
Altug Koc, Ozge Ozer Kaya, Berk Ozyilmaz, Yasar B. Kutbay, Ozgur Kirbiyik, Taha R. Ozdemir, Kadri M. Erdogan, Merve Saka Guvenc, Deniz C. Oztekin, Mehmet Ozeren, Halil G. Pala, Atalay Ekin, Cenk Gezer, Alkim G. Sahingoz Yildirim, Bahar Konuralp Atakul, Secil Kurtulmus, Ugur Turhan, Cuneyt E. Taner   +17 more
doaj   +1 more source

MiSeq assembly statistics for the 3 O26:H11 STECs.

, 2019
MiSeq assembly statistics for the 3 O26:H11 STECs.
Narjol González-Escalona (280578), Eric W. Brown (213283), Marc A. Allard (7067681), Shashi Sharma (282487), Maria Hoffmann (720254)   +4 more
core   +1 more source

HIV Drug Resistance Testing by High-Multiplex “Wide” Sequencing on the MiSeq Instrument [PDF]

, 2015
Limited access to HIV drug resistance testing in low- and middle-income countries impedes clinical decision-making at the individual patient level. An efficient protocol to address this issue must be established to minimize negative therapeutic outcomes ...
Dong, W, Bangsberg, DR, Karakas, A, Kirkby, D, Brumme, CJ, Boum, Y, Lapointe, HR, Poon, AFY, Harrigan, PR, Lee, GQ, Mocello, AR, Martin, JN   +11 more
core   +1 more source

GenomeTrakr WGS Protocol Collection and Workflow for MiSeq v2

, 2022
Here we have created a collection of all the protocols used for WGS using the MiSeq, in order, from sample extraction to NCBI submission. This collection has three sections: Section 1: WGS Wet lab workflow for Illumina MiSeq Section 2: Dry lab workflow for sequence QC and NCBI submission - Direct Submission Section 3: Dry lab workflow for sequence ...
openaire   +1 more source

Effects of Cellulase and Lactobacillus plantarum Supplementation on Fermentation Characteristics, In Situ Degradability, and Microbial Community Dynamics of Apple Pomace Silage

Animal Research and One Health, EarlyView.
This study aimed to investigate the effects of cellulase and Lactobacillus plantarum on the silage quality, in situ digestibility, and microbial communities of apple pomace silage. The combined supplementation of cellulase and Lactobacillus plantarum in apple pomace silage improved fermentation quality and enhanced rumen utilization efficiency.
Zhuangzhuang Liu, Xiongfei Zhang, Fengbo Sun, Junhu Yao, Xinjian Lei   +4 more
wiley   +1 more source