Results 141 to 150 of about 145,034 (286)

Miseq sequence identification of bacteria isolated from Çıldır Lake

, 2021
 In this study, it was aimed to identify and phylogenetic evaluation of bacteria isolated from Çıldır lake sediment. Four sediment sampling were done from two different regions. For bacterial isolations, samples were incubated in Bennet's agar at 28 oC for 7 days.
ÖNALAN, Şükrü, SEÇKİN, Hamdullah
openaire   +4 more sources

Whole-genome and targeted sequencing of drug-resistant Mycobacterium tuberculosis on the iSeq100 and MiSeq: A performance, ease-of-use, and cost evaluation.

PLoS Medicine, 2019
BackgroundAccurate, comprehensive, and timely detection of drug-resistant tuberculosis (TB) is essential to inform patient treatment and enable public health surveillance. This is crucial for effective control of TB globally. Whole-genome sequencing (WGS)
Rebecca E Colman, Aurélien Mace, Marva Seifert, Jonathan Hetzel, Haifa Mshaiel, Anita Suresh, Darrin Lemmer, David M Engelthaler, Donald G Catanzaro, Amanda G Young, Claudia M Denkinger, Timothy C Rodwell   +11 more
doaj   +1 more source

Sample Preparation for Illumina MiSeq Dual Index Amplicon Sequencing v1 [PDF]

, 2019
Amplicon sequencing sample preparation for bacteria and microeukaryotes
Máté Vass, Anna Székely
openaire   +1 more source

Seasonal variation in wild pig (Sus scrofa) diet revealed by DNA metabarcoding

Wildlife Society Bulletin, EarlyView.
Using DNA metabarcoding of fecal samples, we investigated the diet of wild pigs at an extensive bottomland hardwood forest ecosystem within Felsenthal National Wildlife Refuge in Southern Arkansas. We found that wild pig diet was highly diverse and included at least 74 plant families and 106 genera and 23 species of vertebrates.
Kenneth C. Wilson, Scott J. Chiavacci, Caleb P. Roberts, Brett A. DeGregorio   +3 more
wiley   +1 more source

Sequencing of 231 forensic genetic markers using the MiSeq FGx™ forensic genomics system – an evaluation of the assay and software

Forensic Sciences Research, 2018
The MiSeq FGx™ Forensic Genomics System types 231 genetic markers in one multiplex polymerase chain reaction (PCR) assay. The markers include core forensic short tandem repeats (STRs) as well as identity, ancestry and phenotype informative short ...
Christian Hussing, Christina Huber, Rajmonda Bytyci, Helle S. Mogensen, Niels Morling, Claus Børsting   +5 more
doaj   +1 more source

Performance comparison of bench-top next generation sequencers using microdroplet PCR-based enrichment for targeted sequencing in patients with autism spectrum disorder.

PLoS ONE, 2013
Next-generation sequencing (NGS) combined with enrichment of target genes enables highly efficient and low-cost sequencing of multiple genes for genetic diseases.
Eriko Koshimizu, Satoko Miyatake, Nobuhiko Okamoto, Mitsuko Nakashima, Yoshinori Tsurusaki, Noriko Miyake, Hirotomo Saitsu, Naomichi Matsumoto   +7 more
doaj   +1 more source

Postauricular Skin Mycobiome Profiles in Atopic Dermatitis Treated With Dupilumab or Cyclosporine A: A Descriptive Case Series

The Journal of Dermatology, EarlyView.
ABSTRACT Atopic dermatitis (AD) essentially exhibits dysbiosis of skin fungal microbiome, mycobiome, characterized by depletion of Malassezia. The effects of recent systemic therapies for AD on skin mycobiome were not understood enough. We examined changes of skin mycobiome before and after systemic treatments with anti‐IL‐4Rα antibody (dupilumab: DUP)
Yuta Koike, Hitomi Morisaki, Daisuke Motooka, Mai Matsumoto, Motoi Takenaka, Hiroyuki Murota   +5 more
wiley   +1 more source

Comparative analyses of the results of HLA genes typing by NGS method using different platforms

Медицинская иммунология
Next generation sequencing (NGS) allows high-resolution and allelic HLA-typing. The most common platform is MiSeq (Illumina, USA). Currently, the maintenance of this device is difficult, which has necessitated the search for analogues that are not ...
I. E. Pavlova, E. V. Kuzmich, E. G. Khamaganova, D. V. Rudik, E. P. Kuzminova, A. R. Abdrakhimova   +5 more
doaj   +1 more source

Comparison of Two Massively Parallel Sequencing Platforms using 83 Single Nucleotide Polymorphisms for Human Identification

Scientific Reports, 2017
The potential of Massively Parallel Sequencing (MPS) technology to vastly expand the capabilities of human identification led to the emergence of different MPS platforms that use forensically relevant genetic markers.
Dame Loveliness T. Apaga, Sheila E. Dennis, Jazelyn M. Salvador, Gayvelline C. Calacal, Maria Corazon A. De Ungria   +4 more
doaj   +1 more source

SARS-CoV-2 Illumina MiSeq protocol v.2 v2

, 2021
ARTIC amplicon sequencing protocol adapted from Josh Quick's https://www.protocols.io/view/ncov-2019-sequencing-protocol-v2-bdp7i5rn for illumina sequencing of SARS-CoV-2
openaire   +1 more source