Results 141 to 150 of about 147,075 (286)

Sample Preparation for Illumina MiSeq Dual Index Amplicon Sequencing v1 [PDF]

, 2019
Amplicon sequencing sample preparation for bacteria and microeukaryotes
Máté Vass, Anna Székely
openaire   +1 more source

Inulin‐Butyrate Nanogel for Modulation of Gut Microbiome, Intestinal Barrier, and Regulatory T‐Cells in Colitis

Small, EarlyView.
IBD is driven by a vicious cycle of dysbiosis, barrier dysfunction, and immune dysregulation. We developed an inulin–butyrate conjugate nanogel (IBN) that accumulates in inflamed colon and releases butyrate via microbial inulinase/esterase. The inulin shell remodels the microbiome, while localized butyrate strengthens barrier integrity and promotes ...
Nayoon Park, Bom Lee, Hyeon‐Jeong Jeon, Ji Yeon Kim, Suyeon Park, Seong‐Eun Kim, Dong‐Kyu Lee, Yonghyun Lee   +7 more
wiley   +1 more source

Sequencing of 231 forensic genetic markers using the MiSeq FGx™ forensic genomics system – an evaluation of the assay and software

Forensic Sciences Research, 2018
The MiSeq FGx™ Forensic Genomics System types 231 genetic markers in one multiplex polymerase chain reaction (PCR) assay. The markers include core forensic short tandem repeats (STRs) as well as identity, ancestry and phenotype informative short ...
Christian Hussing, Christina Huber, Rajmonda Bytyci, Helle S. Mogensen, Niels Morling, Claus Børsting   +5 more
doaj   +1 more source

Performance comparison of bench-top next generation sequencers using microdroplet PCR-based enrichment for targeted sequencing in patients with autism spectrum disorder.

PLoS ONE, 2013
Next-generation sequencing (NGS) combined with enrichment of target genes enables highly efficient and low-cost sequencing of multiple genes for genetic diseases.
Eriko Koshimizu, Satoko Miyatake, Nobuhiko Okamoto, Mitsuko Nakashima, Yoshinori Tsurusaki, Noriko Miyake, Hirotomo Saitsu, Naomichi Matsumoto   +7 more
doaj   +1 more source

Distinct Nasal Microbiome Profiles and Prediction Model for Allergic Rhinitis, Nonallergic Rhinitis, and Healthy Children

World Journal of Otorhinolaryngology - Head and Neck Surgery, EarlyView.
The alpha and beta diversity of the nasal microbiome differed among children with allergic rhinitis (AR), nonallergic rhinitis (NAR), and healthy controls (HCs). Compared to HC, AR had more Escherichia‐Shigella, Negativicoccus, and Campylobacter, while NAR had more Dolosigranulum and fewer Enterobacteriaceae.
Kantima Kanchanapoomi, Iyarit Thaipisuttikul, Perapon Nitayanon, Witchaya Srisuwatchari, Punchama Pacharn, Nualanong Visitsunthorn, Orathai Jirapongsananuruk   +6 more
wiley   +1 more source

Comparative analyses of the results of HLA genes typing by NGS method using different platforms

Медицинская иммунология
Next generation sequencing (NGS) allows high-resolution and allelic HLA-typing. The most common platform is MiSeq (Illumina, USA). Currently, the maintenance of this device is difficult, which has necessitated the search for analogues that are not ...
I. E. Pavlova, E. V. Kuzmich, E. G. Khamaganova, D. V. Rudik, E. P. Kuzminova, A. R. Abdrakhimova   +5 more
doaj   +1 more source

Comparison of Two Massively Parallel Sequencing Platforms using 83 Single Nucleotide Polymorphisms for Human Identification

Scientific Reports, 2017
The potential of Massively Parallel Sequencing (MPS) technology to vastly expand the capabilities of human identification led to the emergence of different MPS platforms that use forensically relevant genetic markers.
Dame Loveliness T. Apaga, Sheila E. Dennis, Jazelyn M. Salvador, Gayvelline C. Calacal, Maria Corazon A. De Ungria   +4 more
doaj   +1 more source

SARS-CoV-2 Illumina MiSeq protocol v.2 v2

, 2021
ARTIC amplicon sequencing protocol adapted from Josh Quick's https://www.protocols.io/view/ncov-2019-sequencing-protocol-v2-bdp7i5rn for illumina sequencing of SARS-CoV-2
openaire   +1 more source

Necromass chemistry drives the functional diversity of the necrobiome, resulting in microbe–organic matter feedbacks

Functional Ecology, EarlyView.
Read the free Plain Language Summary for this article on the Journal blog. Abstract In temperate European forests, soil fungal communities, dominated by saprotrophic and ectomycorrhizal (ECM) species, represent almost 25% of soil organic carbon (C) in the soil.
Elsa Hilaire, Laure Fauchery, Michaël Danger, Aurélie Cébron, Bernhard Zeller, Marc Buée   +5 more
wiley   +1 more source

Is the endophyte‐based plant protection against aphids mediated by changes in the insect microbiome?

Insect Science, EarlyView.
We showed that the fungal endophyte Epichloë strain AR37 increased the plant resistance against aphids via the production of indole diterpene alkaloids. The reduction in aphid performance was not associated with changes in abundance/composition/diversity of the insect's bacterial microbiota, but additional endophyte effects on this microbiota cannot be
Daniel A. Bastías, Luis Carvalho, Ruy Jáuregui, Richard D. Johnson, Wei Zhang, Pedro E. Gundel   +5 more
wiley   +1 more source