Results 81 to 90 of about 145,034 (286)

Comparison of Pre‐ and Postoperative Gut Microbiota Diversity in Patients With Rectal Cancer Undergoing Stoma Creation and Closure

open access: yesAnnals of Gastroenterological Surgery, EarlyView.
We investigated the impact of temporary stoma creation and closure on gut microbiota diversity in rectal cancer patients. 16S rRNA sequencing revealed that microbial diversity significantly decreased postoperatively in the stoma group but remained stable in non‐stoma patients.
Yusuke Suzuki   +9 more
wiley   +1 more source

Towards clinical molecular diagnosis of inherited cardiac conditions: a comparison of bench-top genome DNA sequencers.

open access: yesPLoS ONE, 2013
BackgroundMolecular genetic testing is recommended for diagnosis of inherited cardiac disease, to guide prognosis and treatment, but access is often limited by cost and availability.
Xinzhong Li   +11 more
doaj   +1 more source

Draft genome sequence of Pseudomonas aeruginosa strain Hex1T isolated from soils contaminated with used lubricating oil in Argentina [PDF]

open access: yes, 2017
Pseudomonas aeruginosa Hex1T was isolated from soils contaminated with used lubricating oil from a garage in Córdoba, Argentina. This strain is capable of utilizing this pollutant as the sole carbon and energy source.
Feliziani, Sofía   +2 more
core   +1 more source

Nextera XT protocol for MiSeq HIVPR-RT sequencing v1

open access: yes, 2021
The protocol allows amplification and NGS sequencing of a PR-RT HIV-1 sequence using tagmentation strategy and Illumina MiSeq equipment, followed by analysis of drug-resistance associated mutations by HyDRA
openaire   +1 more source

De novo yeast genome assemblies from MinION, PacBio and MiSeq platforms [PDF]

open access: yesScientific Reports, 2017
AbstractLong-read sequencing technologies such as Pacific Biosciences and Oxford Nanopore MinION are capable of producing long sequencing reads with average fragment lengths of over 10,000 base-pairs and maximum lengths reaching 100,000 base- pairs. Compared with short reads, the assemblies obtained from long-read sequencing platforms have much higher ...
Francesca Giordano   +13 more
openaire   +4 more sources

Dietary Baicalin Supplementation Can Enhance the Growth Performance of Weaned Piglets and Maintain the Intestinal Barrier Integrity

open access: yesAnimal Research and One Health, EarlyView.
This research innovatively illustrates baicalin’s multifaceted mechanisms in enhancing piglets’ intestinal health: modulating bile acid metabolism via probiotics, reinforcing tight junction proteins (ZO‐1/claudin), suppressing TLR4/NF‐κB‐mediated inflammation, whereas promoting growth and reducing diarrhea.
Yuhui Gao   +6 more
wiley   +1 more source

Protocol for Oligo pool sequencing on MiSeq v1

open access: yes, 2022
The protocol is modified based on Illumina's guidance. It is used to prepare oligo-pool samples for sequencing. 30 % of Phix is recommended. Sequencing cycles and kits can be adjusted based on the size of the library.
openaire   +1 more source

Effects of Lycium barbarum Residue Substituting Dietary Maize on Fecal Microbiota and Growth Performance of Crossbred Simmental Cattle

open access: yesAnimal Research and One Health, EarlyView.
The bioactive substances we extracted from Lycium barbarum residue (LBR) have a typical polysaccharide structure. When 1.80% LBR was used to replace maize in the diet, the average daily gain of rossbred Simmental was significantly increased, and anti‐inflammatory ability was improved. In addition, LBR improved the fecal microbial composition, increased
Kun Cai   +10 more
wiley   +1 more source

Microbial communities and arsenic biogeochemistry at the outflow of an alkaline sulfide-rich hot spring. [PDF]

open access: yes, 2016
Alkaline sulfide-rich hot springs provide a unique environment for microbial community and arsenic (As) biogeochemistry. In this study, a representative alkaline sulfide-rich hot spring, Zimeiquan in the Tengchong geothermal area, was chosen to study ...
Dai, Xinyue   +9 more
core   +1 more source

Investigation on the Genetic-Inconsistent Paternity Cases Using the MiSeq FGx System

open access: yesForensic Sciences Research, 2022
AbstractMutations might challenge the paternity index calculation in forensic identification. While many studies have focussed on the autosomal short tandem repeats (A-STR), the mutation status of sex chromosomes and single nucleotide polymorphism (SNP) remain blank.
Anqi Chen   +3 more
openaire   +3 more sources

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