Results 51 to 60 of about 57,121 (194)

dsRADAR: Imaging and Detecting Cellular dsRNA by Repurposing RNA‐Binding Proteins

Angewandte Chemie, EarlyView.
Double‐stranded RNA (dsRNA) plays an important role in the innate immune system, but can be misregulated in disease, leading to inflammation. Despite the importance of this motif, methods for detecting dsRNA remain limited. This work shows that ADAR3 can be repurposed to provide improved detection of dsRNA, which is demonstrated in the context of viral
Weina Cheng, Tyson D. Todd, Alexandria L. Quillin, Harshad Ingle, Angela M. Halstead, Megan T. Baldridge, José B. Sáenz, Jennifer M. Heemstra   +7 more
wiley   +2 more sources

AlphaFold prediction of structural ensembles of disordered proteins

Nature Communications
Deep learning methods of predicting protein structures have reached an accuracy comparable to that of high-resolution experimental methods. It is thus possible to generate accurate models of the native states of hundreds of millions of proteins.
Z. Faidon Brotzakis, Shengyu Zhang, Mhd Hussein Murtada, Michele Vendruscolo   +3 more
doaj   +1 more source

Lipid Metabolic Dysregulation Driven by OSMR Mutations Underlies Amyloidogenesis in Primary Localized Cutaneous Amyloidosis

Advanced Science, EarlyView.
OSMR mutations disrupt STAT5 signaling and AKR1B10‐mediated lipid metabolism, leading to lipid accumulation and protein aggregation in keratinocytes. This metabolic reprogramming drives amyloid deposition in primary localized cutaneous amyloidosis, revealing a mechanistic link between lipid dysregulation and amyloidogenesis.
Huiting Liu, Wen Zheng, Chao Yang, Tao Yang, Qiuyu Chen, Ping Lu, Huan Yang, Jun Liu, Bin Yang   +8 more
wiley   +1 more source

Adaptation of a Fluorogenic RNA to N1‐Methylpseudouridine Nucleoside Chemistry

Angewandte Chemie, EarlyView.
Several green‐emitting fluorogenic RNA aptamers were evaluated following the replacement of every uridine by N1‐methylpseudouridine (mΨ). Because this modification impaired the function of most aptamers, a directed evolution strategy combining droplet microfluidics, bioinformatics, and rational design was used to identify an aptamer preserving its ...
Patrick P.A. Bertrand, Roger Cubi, Claire Husser, Magali Frugier, Michael Ryckelynck   +4 more
wiley   +2 more sources

Decoding Biomolecular Phase Separation: From Physiological Roles to Pathological Mechanisms and Therapeutic Biomaterials Design

Advanced Science, EarlyView.
This review systematically elaborates on the mechanisms underlying physiological regulation and pathological dysregulation of biomolecular phase separation. It also summarizes advances in phase‑separation‑targeted therapeutic strategies and the design of functional biomaterials inspired by this process.
Jiawei Zhu, Jingbo Dong, Qiufang Gong, Xuejiao Song, Zhifa Shen, Chao Liang   +5 more
wiley   +1 more source

Bacteria‐Derived 1‐Allylpiperazine Alleviates Symptoms of Parkinson's Disease Model by Promoting Autophagy

Advanced Science, EarlyView.
Parkinson's disease currently has no disease‐modifying therapies. In Caenorhabditis elegans, Escherichia coli E8 alleviates α‐synuclein‐provoked PD‐related defects through its bioactive metabolite 1‐ALPP, which combats PD by triggering AMPK/mTOR/HLH‐30‐dependent autophagy and suppressing α‐synuclein LLPS‐mediated fibrillization.
Mingqin Qu, Xinyuan Zhang, Xinyi Chen, Jiaxin Shi, Meng Xu, Fuhao Wang, Jinling Lv, Jiaman Sun, Weiyi Niu, Zihan Liu, Li Li, Fufeng Liu, Xiangming Wang   +12 more
wiley   +1 more source

Rapid elongation drives the exceptionally fast aggregation of the most common localized human amyloid medin

Communications Chemistry
Amyloid deposition is a hallmark of numerous age-related diseases, and understanding the chemical mechanisms that govern amyloid formation is crucial for advancing the rational development of protein aggregation inhibitors.
Vaidehi Roy Chowdhury, Robert I. Horne, Mariana P. Cali, Zenon Toprakcioglu, Sara Linse, Michele Vendruscolo   +5 more
doaj   +1 more source

Prediction of protein biophysical traits from limited data: a case study on nanobody thermostability through NanoMelt

mAbs
In-silico prediction of protein biophysical traits is often hindered by the limited availability of experimental data and their heterogeneity. Training on limited data can lead to overfitting and poor generalizability to sequences distant from those in ...
Aubin Ramon, Mingyang Ni, Olga Predeina, Rebecca Gaffey, Patrick Kunz, Shimobi Onuoha, Pietro Sormanni   +6 more
doaj   +1 more source

Injectable Peptide‐Based Functional Amyloid Hydrogels as Tunable Depots for Sustained Drug Delivery

Advanced Science, EarlyView.
An injectable and tunable peptide‐based functional amyloid hydrogel provides sustained insulin release while preserving insulin stability and bioactivity. The hydrogel enables prolonged glycemic regulation in vivo, exhibits excellent biocompatibility, and shows no detectable IAPP and α‐Syn heterologous cross‐seeding, highlighting its potential as a ...
Ranjit Shaw, Niyamat M. A. Chimthanawala, Shouvik Manna, Sadhana Sathaye, Samir K. Maji   +4 more
wiley   +1 more source

Animal models for investigating post‐stroke cognitive impairment: A neurovascular trafficking perspective

Animal Models and Experimental Medicine, EarlyView.
Animal models enable mechanistic testing of how ischemic injury disrupts neurovascular trafficking and drives cognitive vulnerability after stroke. Complementary models capture distinct vascular, cellular, white matter, and cognitive features of PSCI.
Pratchaya Kaewkaen
wiley   +1 more source