Results 61 to 70 of about 57,121 (194)

Single-molecule digital sizing of proteins in solution

Nature Communications
The physical characterization of proteins in terms of their sizes, interactions, and assembly states is key to understanding their biological function and dysfunction.
Georg Krainer, Raphael P. B. Jacquat, Matthias M. Schneider, Timothy J. Welsh, Jieyuan Fan, Quentin A. E. Peter, Ewa A. Andrzejewska, Greta Šneiderienė, Magdalena A. Czekalska, Hannes Ausserwoeger, Lin Chai, William E. Arter, Kadi L. Saar, Therese W. Herling, Titus M. Franzmann, Vasilis Kosmoliaptsis, Simon Alberti, F. Ulrich Hartl, Steven F. Lee, Tuomas P. J. Knowles   +19 more
doaj   +1 more source

Mechanisms of mutant SOD1 induced mitochondrial toxicity in amyotrophic lateral sclerosis

Frontiers in Cellular Neuroscience, 2014
In amyotrophic lateral sclerosis (ALS) mitochondrial dysfunction is recognized as one of the key elements contributing to the pathology. Mitochondria are the major source of intracellular reactive oxygen species (ROS). Increased production of ROS as well
Piia eVehviläinen, Jari eKoistinaho, Gundars eGoldsteins   +2 more
doaj   +1 more source

Blood SOD1 Activity in ALS Patients Receiving Tofersen Treatment

Annals of Neurology, EarlyView.
Objective The antisense oligonucleotide tofersen is the first disease‐modifying drug for SOD1‐related amyotrophic lateral sclerosis (ALS) and was approved because of its ability to reduce SOD1 protein and neurofilament levels. The effect of tofersen on SOD1 activity is unclear but of clinical relevance because homozygous SOD1 mutations, linked to ...
Katharina Goehring, Enya Abler, Jakub Vavra, Kristina Mayer, Johannes Dorst, Zeynep Elmas, Maximilian Wiesenfarth, Lars Richter, Susanne Petri, Martin Regensburger, Peter Reilich, Bogdan Bjelica, Rea Lumi, Patrick Süß, Hayrettin Tumani, Markus Otto, Jochen H. Weishaupt, Albert C. Ludolph, Patrick Oeckl   +18 more
wiley   +1 more source

Developability engineering of scFvs enables robust CAR function under transient mRNA expression

Scientific Reports
Lipid nanoparticle delivery of mRNA enables in vivo generation of CAR-T cells while avoiding viral vector manufacturing constraints. However, transient expression places stringent requirements on the developability of CAR components, particularly single ...
Maurizio Mangolini, Aubin Ramon, Bingqian Li, Emily Souster, Jasvinder Hayre, Rajesh Karattil, Pietro Sormanni, Shimobi Onuoha   +7 more
doaj   +1 more source

GroEL dependency affects codon usage—support for a critical role of misfolding in gene evolution

Molecular Systems Biology, 2010
It has recently been suggested that the use of optimal codons limits mistranslation‐induced protein misfolding, yet evidence for this remains largely circumstantial.
Tobias Warnecke, Laurence D Hurst
doaj   +1 more source

Chitosan-myristate nanogel as an artificial chaperone protects neuroserpin from misfolding

Advanced Biomedical Research, 2016
Background: Molecular chaperon-like activity for protein refolding was studied using nanogel chitosan-myristic acid (CMA) and the protein neuroserpin (NS), a member of the serine proteinase inhibitor superfamily (serpin).
Habib Nazem, Afshin Mohsenifar, Sahar Majdi   +2 more
doaj   +1 more source

Publisher Correction: Two human metabolites rescue a C. elegans model of Alzheimer’s disease via a cytosolic unfolded protein response

Communications Biology, 2021
Priyanka Joshi, Michele Perni, Ryan Limbocker, Benedetta Mannini, Sam Casford, Sean Chia, Johnny Habchi, Johnathan Labbadia, Christopher M. Dobson, Michele Vendruscolo   +9 more
doaj   +1 more source

Disulfide Bond–Modified Proteomics Reveals the Effects of Riboflavin on Protein Folding Dynamics

Animal Research and One Health, EarlyView.
Riboflavin deficiency impairs oxidative protein folding, causing ER misfolded protein accumulation, CHOP activation, and apoptosis, revealing a role of riboflavin in proteostasis. ABSTRACT Riboflavin is a crucial micronutrient essential for maintaining cellular homeostasis, acting as an important precursor for flavoproteins that utilize flavin ...
Bo Zhang, Shuisheng Hou, Jing Tang
wiley   +1 more source

Protein thermal stability in the undergraduate biochemistry laboratory: Exploring protein thermal stability with yeast alcohol dehydrogenase

Biochemistry and Molecular Biology Education, Volume 53, Issue 2, Page 209-217, March/April 2025.
Abstract We created a novel laboratory experience where undergraduate students explore the techniques used to study protein misfolding, unfolding, and aggregation. Despite the importance of protein misfolding and aggregation diseases, protein unfolding is not typically explored in undergraduate biochemistry laboratory classes.
Alison Bates, Kathryn M. Williams, Ann E. Hagerman   +2 more
wiley   +1 more source

Bridging the translational gap in clinical nanomedicine: From rational design to clinical reality

BMEMat, EarlyView.
Nanotechnology offers a multi‐faceted approach to modern healthcare, encompassing high‐sensitivity diagnostic imaging, innovative vaccine delivery, and targeted therapeutics. This review explores how these nanomedicine applications address critical challenges in cancer recurrence and the treatment of neurologic and respiratory diseases to combat rising
Ahmed H. Ghonaim, Marwa Ahmed El‐Naka, Wedad M. Nageeb, Atta Ullah Khan, Noura M. Seleem, Aliaa Abdelghafar, Doaa Zamel, Yang Liu, Qigai He, Wentao Li   +9 more
wiley   +1 more source