Results 91 to 100 of about 3,064,383 (240)

Unit of analysis issues in laboratory-based research

eLife, 2018
Many studies in the biomedical research literature report analyses that fail to recognise important data dependencies from multilevel or complex experimental designs.
Nick R Parsons, M Dawn Teare, Alice J Sitch   +2 more
doaj   +1 more source

Structural insights into an engineered feruloyl esterase with improved MHET degrading properties

FEBS Letters, EarlyView.
A feruloyl esterase was engineered to mimic key features of MHETase, enhancing the degradation of PET oligomers. Structural and computational analysis reveal how a point mutation stabilizes the active site and reshapes the binding cleft, expading substrate scope.
Panagiota Karampa, Konstantinos Makryniotis, Theofani‐Iosifina Sousani, Evangelos Topakas, Vangelis Daskalakis, Maria Dimarogona   +5 more
wiley   +1 more source

lmerTest Package: Tests in Linear Mixed Effects Models

Journal of Statistical Software, 2017
One of the frequent questions by users of the mixed model function lmer of the lme4 package has been: How can I get p values for the F and t tests for objects returned by lmer?
Alexandra Kuznetsova, Per B. Brockhoff, Rune H. B. Christensen   +2 more
doaj   +1 more source

Gut microbiome and aging—A dynamic interplay of microbes, metabolites, and the immune system

FEBS Letters, EarlyView.
Age‐dependent shifts in microbial communities engender shifts in microbial metabolite profiles. These in turn drive shifts in barrier surface permeability of the gut and brain and induce immune activation. When paired with preexisting age‐related chronic inflammation this increases the risk of neuroinflammation and neurodegenerative diseases.
Aaron Mehl, Eran Blacher
wiley   +1 more source

Variational Bayesian EM Algorithm for Quantile Regression in Linear Mixed Effects Models

Mathematics
This paper extends the normal-beta prime (NBP) prior to Bayesian quantile regression in linear mixed effects models and conducts Bayesian variable selection for the fixed effects of the model.
Weixian Wang, Maozai Tian
doaj   +1 more source

A methionine‐lined active site governs carbocation stabilization and product specificity in a bacterial terpene synthase

FEBS Letters, EarlyView.
This study reveals a unique active site enriched in methionine residues and demonstrates that these residues play a critical role by stabilizing carbocation intermediates through novel sulfur–cation interactions. Structure‐guided mutagenesis further revealed variants with significantly altered product profiles, enhancing pseudopterosin formation. These
Marion Ringel, Carl P. O. Helmer, Shani Zev, Ronja Driller, Emily Buhr, Markus Reinbold, Renana Schwartz, Gabriel Foley, Mikael Boden, Daniel Garbe, Gerhard Schenk, Dan Thomas Major, Bernhard Loll, Thomas Brück   +13 more
wiley   +1 more source

Occupational stress and body composition of hospital workers: a follow-up study

Frontiers in Public Health
This study sought to analyze the influence of occupational stress on the body composition of hospital workers after one year of follow-up. This prospective cohort study included 218 workers from one of the leading private hospitals in the municipality of
Carlos Rodrigo Nascimento de Lira, Rita de Cássia Coelho de Almeida Akutsu, Lorene Gonçalves Coelho, Renata Puppin Zandonadi, Priscila Ribas de Farias Costa   +4 more
doaj   +1 more source

Valosin‐containing protein counteracts ATP‐driven dissolution of FUS condensates through its ATPase activity in vitro

FEBS Letters, EarlyView.
Biomolecular condensates formed by fused in sarcoma (FUS) are dissolved by high ATP concentrations yet persist in cells. Using a reconstituted system, we demonstrate that valosin‐containing protein (VCP), an AAA+ ATPase, counteracts ATP‐driven dissolution of FUS condensates through its D2 ATPase activity.
Hitomi Kimura, Shin‐ichi Tate, Kyota Yasuda   +2 more
wiley   +1 more source

Linear Mixed Models: Gum and Beyond

Measurement Science Review, 2014
In Annex H.5, the Guide to the Evaluation of Uncertainty in Measurement (GUM) [1] recognizes the necessity to analyze certain types of experiments by applying random effects ANOVA models.
Arendacká Barbora, Täubner Angelika, Eichstädt Sascha, Bruns Thomas, Elster Clemens   +4 more
doaj   +1 more source

Diversity and complexity in neural organoids

FEBS Letters, EarlyView.
Neural organoid research aims to expand genetic diversity on one side and increase tissue complexity on the other. Chimeroids integrate multiple donor genomes within single organoids. Self‐organising multi‐identity organoids, exogenous cell seeding, or enforced assembly of region‐specific organoids contribute to tissue complexity.
Ilaria Chiaradia, Madeline A. Lancaster
wiley   +1 more source