Results 181 to 190 of about 4,649,595 (248)

Sustainable Valorization of Grape Pomace Digestate as Fertilizer: Effects on the Agronomic and Biochemical Performance of <i>Calendula officinalis</i> L. under Variable Irrigation Regimes. [PDF]

ACS Omega
Castro LEN, Mazzini-Guedes RB, Ribeiro MD, Matheus LR, de Souza DLQ, Ribeiro LF, Colpini LMS, Forster-Carneiro T.   +7 more
europepmc   +1 more source

Automated FRAP microscopy for high‐throughput analysis of protein dynamics in chromatin organization and transcription

FEBS Open Bio, EarlyView.
RoboMic is an automated confocal microscopy pipeline for high‐throughput functional imaging in living cells. Demonstrated with fluorescence recovery after photobleaching (FRAP), it integrates AI‐driven nuclear segmentation, ROI selection, bleaching, and analysis.
Selçuk Yavuz, Bart Geverts, Johan A. Slotman, Andrea Sacchetti, Stefan Prekovic, Martin E. van Royen, Adriaan B. Houtsmuller   +6 more
wiley   +1 more source

Recombinant Protein Expression in Rhodococcus species. [PDF]

Curr Protoc
Akintubosun M, Higgins MA.
europepmc   +1 more source

Polarization‐resolved femtosecond Vis/IR spectroscopy tailored for resolving weak signals in biological samples using minimal sample volume

FEBS Open Bio, EarlyView.
Unique biological samples, such as site‐specific mutant proteins, are available only in limited quantities. Here, we present a polarization‐resolved transient infrared spectroscopy setup with referencing to improve signal‐to‐noise tailored towards tracing small signals. We provide an overview of characterizing the excitation conditions for polarization‐
Clark Zahn, Karsten Heyne
wiley   +1 more source

Development and validation of HPLC-FLD method for simultaneous determination of ternary therapy used for COVID-19 in plasma samples. [PDF]

Sci Rep
Madbouly EA, El-Shanawani AA, El-Adl SM, Abdelkhalek AS.   +3 more
europepmc   +1 more source

A light‐triggered Time‐Resolved X‐ray Solution Scattering (TR‐XSS) workflow with application to protein conformational dynamics

FEBS Open Bio, EarlyView.
Time‐resolved X‐ray solution scattering captures how proteins change shape in real time under near‐native conditions. This article presents a practical workflow for light‐triggered TR‐XSS experiments, from data collection to structural refinement. Using a calcium‐transporting membrane protein as an example, the approach can be broadly applied to study ...
Fatemeh Sabzian‐Molaei, Fredrik Orädd, Konstantinos Magkakis, Magnus Andersson   +3 more
wiley   +1 more source

Comprehensive Phytochemical Profiling and Multi-Target Biological Evaluation of <i>Hibiscus sabdariffa</i> L. Calyx Extracts From Libya: A Promising Functional Food Candidate With Antibacterial, Antioxidant, Anti-Inflammatory, and Cytotoxic Properties: Validation Through In Silico Approaches. [PDF]

Food Sci Nutr
Kabbashi AS, Mussa Akrim ZS, Suliman MB, El-Hddad SSA, Mustafa AA, Ibrahim MM, Ibrahim ER, Mosa MR, Hasan RB.   +8 more
europepmc   +1 more source

Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies

FEBS Open Bio, EarlyView.
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli, Mohammad Nour Alsamsam, Ugnė Bagdonaitė, Van Truc Vu, Chun‐Jen Huang, Polina Kuzhir, Mindaugas Zaremba, Aurimas Kopūstas, Marijonas Tutkus   +8 more
wiley   +1 more source

Primary Yolk Sac Tumor of the Endometrium: A Case Report and Comprehensive Literature Review. [PDF]

Case Rep Pathol
Cheng H, Song Y, Shi SS.
europepmc   +1 more source