Results 91 to 100 of about 636,952 (296)
Screening and epitope characterization of Nidogen‐2‐specific nanobodies
Camel immunization and phage display were employed to generate high‐affinity VHH nanobodies against Nidogen‐2. After library construction, biopanning, ELISA screening, sequencing, and recombinant expression, selected nanobodies were purified and characterized, leading to the preliminary exploration of a nanobody‐based sandwich ELISA for specific ...
Jianchuan Wen +9 more
wiley +1 more source
Mass spectrometry based identification of AMP‐O‐Tris generated by Thermococcus onnurineus Cas10
Isolated Thermococcus onnurineus Cas10 generates the noncanonical ATP‐derived product AMP‐O‐Tris while in Tris‐containing buffer as identified via mass spectrometry, revealing relaxed nucleophile selectivity under isolated conditions. These findings suggest that multiprotein Csm complex assembly restricts Cas10 reactivity toward canonical cyclic ...
Su‐Jin Lee +6 more
wiley +1 more source
The MRP4 transporter exports several drugs and signaling molecules. Here, we identified key promoter elements regulating basal MRP4 expression. Using reporter assays, we defined a conserved region with essential Sp1 and contributory Ets sites, which controlled basal MRP4 expression.
Debora Singer +7 more
wiley +1 more source
During the past dozen years, several mobile mapping systems based on the use of imaging and positioning sensors mounted on terrestrial (and aerial) vehicles have been developed.
Andrea Masiero +5 more
doaj +1 more source
Information Acquisition with Sensing Robots: Algorithms and Error Bounds
Utilizing the capabilities of configurable sensing systems requires addressing difficult information gathering problems. Near-optimal approaches exist for sensing systems without internal states.
Atanasov, Nikolay +3 more
core +1 more source
Evolutionary analysis across 32 placental mammals identified positive selection at residues H148 and W149 in the immune receptor FcγR1. Ancestral reconstruction combined with molecular dynamics simulations reveals how these mutations may influence receptor structure and dynamics, providing insight into the evolution of antibody recognition and immune ...
David A. Young +7 more
wiley +1 more source
Effective Collaborative Mapping Based on Mobile Devices
There are numerous situations when it is utmost important to share efficiently some spatial data among a group of people. Floods can be taken as an obvious example.
Ivo Pisařovic +5 more
doaj +1 more source
An assistive robotics control system based on speech semantic recognition [PDF]
Since 90’s era, many researchers and organizations are working on assistive technologies to ease the disabilities people to move around freely, independence, comfort and have the capabilities to enjoy life to the fullest.
Mohamad, Siti Nur Ateeqa
core
Early‐life exposure to a high‐fat diet altered intact Achilles tendons in rat offspring, making them thinner, stiffer, and molecularly distinct even without injury. These findings suggest that developmental high‐fat diet exposure may impair tendon quality and increase susceptibility to mechanical overload or tendon injury later in life.
Heyong Yin +3 more
wiley +1 more source
Cloud-Based Geospatial 3D Image Spaces—A Powerful Urban Model for the Smart City
In this paper, we introduce the concept and an implementation of geospatial 3D image spaces as new type of native urban models. 3D image spaces are based on collections of georeferenced RGB-D imagery.
Stephan Nebiker +2 more
doaj +1 more source

