Results 181 to 190 of about 279 (205)
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Identification of Phloem Mobile mRNAs Using the Solanaceae Heterograft System
2019Large numbers of mRNAs move in the phloem and some may function as signals to exert important physiological functions in the distal recipient organs. Generating an authentic list of phloem mobile mRNA is a prerequisite for elucidating their physiological functions. Nicotiana benthamiana can be used as a scion to graft on a tomato (Solanum lycopersicum)
Chao, Xia +4 more
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Detection of sRNA–mRNA Interactions by Electrophoretic Mobility Shift Assay
2012Electrophoretic mobility shift assay is a simple, rapid, and sensitive technique to analyze the RNA-RNA interaction. A (32)P-labeled RNA is incubated with another unlabeled RNA and subjected to electrophoresis on a native polyacrylamide gel. If two RNA molecules base pair stably, the movement of the probe RNA through the gel is retarded resulting in a ...
Teppei, Morita +2 more
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Mutational Analysis of sRNA–mRNA Base Pairing by Electrophoretic Mobility Shift Assay
2018Small regulatory RNAs (sRNAs) in bacteria often act by base pairing to mRNAs. Direct interactions between an sRNA and its target mRNA can be investigated by electrophoretic mobility shift assay. In this assay, regions engaged in base pairing are analyzed by introducing mutations in one of the RNAs that prevent sRNA-mRNA complex formation, followed by ...
Lillebæk, Eva Maria Sternkopf +1 more
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Arabidopsis cyclophilins direct intracellular transport of mobile mRNA via organelle hitchhiking
Nature PlantsPlants convert external cues into mobile mRNAs to synchronize meristematic differentiation with environmental dynamics. These mRNAs are selectively transported to intercellular pores, plasmodesmata (PD), for cell-to-cell movement. However, how plants recognize and deliver mobile mRNAs to PD remains unknown.
Kai-Ren Luo +4 more
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Molecular Brain Research, 1994
Functional expression of opioid receptors was detected in the Xenopus oocyte translation system by a voltage-clamp recording. After injection of poly(A)+ RNA isolated from 3-week-old rat striatum or whole brain, the oocytes often demonstrated intracellular Ca(2+)-mediated oscillatory responsiveness to [D-Ala2, N-methyl-Phe4, Gly5-ol]enkephalin (DAMGO),
S, Kaneko +3 more
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Functional expression of opioid receptors was detected in the Xenopus oocyte translation system by a voltage-clamp recording. After injection of poly(A)+ RNA isolated from 3-week-old rat striatum or whole brain, the oocytes often demonstrated intracellular Ca(2+)-mediated oscillatory responsiveness to [D-Ala2, N-methyl-Phe4, Gly5-ol]enkephalin (DAMGO),
S, Kaneko +3 more
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Investigating the Effect of 5’ and 3’ UTRs on mRNA Mobility in Plants
In tomato rootstock and Nicotiana benthamiana heterografting systems, N. benthamiana scions show stress and defense-related transcripts derived from tomato rootstock. tRNA-like and poly-pyrimidine sequences in mRNA untranslated regions (UTRs) may facilitate mRNA mobility.Lin, Judith, Koretsky, Michael
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Plant Molecular Biology, 1993
A cDNA clone encoding an HMG1 protein from Pharbitis nil was characterized with regard to its sequence, genomic organization and regulation in response to photoperiodic treatments that control floral induction. The HMG1 cDNA contains an open reading frame of 432 nucleotides encoding a 144 amino acid protein of approximately 16 kDa.
C C, Zheng, A Q, Bui, S D, O'Neill
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A cDNA clone encoding an HMG1 protein from Pharbitis nil was characterized with regard to its sequence, genomic organization and regulation in response to photoperiodic treatments that control floral induction. The HMG1 cDNA contains an open reading frame of 432 nucleotides encoding a 144 amino acid protein of approximately 16 kDa.
C C, Zheng, A Q, Bui, S D, O'Neill
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Mobile Host mRNAs Are Translated to Protein in the Associated Parasitic Plant Cuscuta campestris
Plants, 2022James Westwood, Koh Aoki, So-Yon Park
exaly
Phloem-Mobile MYB44 Negatively Regulates Expression of PHOSPHATE TRANSPORTER 1 in Arabidopsis Roots
Plants, 2023Leon Kochian +2 more
exaly
Self-demixing of mRNA copies buffers mRNA:mRNA and mRNA:regulator stoichiometries
Cell, 2023Agnes Loubat
exaly