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Identification of phloem-mobile mRNA
Journal of Plant Research, 2014Signaling between cells, tissues and organs is essential for multicellular organisms to coordinate and adapt their development and growth to internal and environmental changes. Plants have evolved a plant-specific symplasmic pathway, called plasmodesmata, for efficient intercellular communication, in addition to the receptor-ligand-based apoplasmic ...
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Science's STKE, 2007
Tuber formation in potato plants is promoted when plants are exposed to short-day photoperiods and inhibited in plants that experience summerlike long periods of daylight. This process requires that a signal be passed from the leaves to the growing tuber underground. Banerjee et al .
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Tuber formation in potato plants is promoted when plants are exposed to short-day photoperiods and inhibited in plants that experience summerlike long periods of daylight. This process requires that a signal be passed from the leaves to the growing tuber underground. Banerjee et al .
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Identification of Phloem Mobile mRNAs Using the Solanaceae Heterograft System
2019Large numbers of mRNAs move in the phloem and some may function as signals to exert important physiological functions in the distal recipient organs. Generating an authentic list of phloem mobile mRNA is a prerequisite for elucidating their physiological functions. Nicotiana benthamiana can be used as a scion to graft on a tomato (Solanum lycopersicum)
Chao, Xia +4 more
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Detection of sRNA–mRNA Interactions by Electrophoretic Mobility Shift Assay
2012Electrophoretic mobility shift assay is a simple, rapid, and sensitive technique to analyze the RNA-RNA interaction. A (32)P-labeled RNA is incubated with another unlabeled RNA and subjected to electrophoresis on a native polyacrylamide gel. If two RNA molecules base pair stably, the movement of the probe RNA through the gel is retarded resulting in a ...
Teppei, Morita +2 more
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The mRNA of a Knotted1-like transcription factor of potato is phloem mobile
Plant Molecular Biology, 2012Potato Homeobox1 (POTH1) is a Knotted1-like transcription factor from the Three Amino Acid Loop Extension (TALE) superfamily that is involved in numerous aspects of development in potato (Solanum tuberosum L). POTH1 interacts with its protein partner, StBEL5, to facilitate binding to specific target genes to modulate hormone levels, mediate leaf ...
Ameya, Mahajan +4 more
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Mutational Analysis of sRNA–mRNA Base Pairing by Electrophoretic Mobility Shift Assay
2018Small regulatory RNAs (sRNAs) in bacteria often act by base pairing to mRNAs. Direct interactions between an sRNA and its target mRNA can be investigated by electrophoretic mobility shift assay. In this assay, regions engaged in base pairing are analyzed by introducing mutations in one of the RNAs that prevent sRNA-mRNA complex formation, followed by ...
Lillebæk, Eva Maria Sternkopf +1 more
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Arabidopsis cyclophilins direct intracellular transport of mobile mRNA via organelle hitchhiking
Nature PlantsPlants convert external cues into mobile mRNAs to synchronize meristematic differentiation with environmental dynamics. These mRNAs are selectively transported to intercellular pores, plasmodesmata (PD), for cell-to-cell movement. However, how plants recognize and deliver mobile mRNAs to PD remains unknown.
Kai-Ren Luo +4 more
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Molecular Brain Research, 1994
Functional expression of opioid receptors was detected in the Xenopus oocyte translation system by a voltage-clamp recording. After injection of poly(A)+ RNA isolated from 3-week-old rat striatum or whole brain, the oocytes often demonstrated intracellular Ca(2+)-mediated oscillatory responsiveness to [D-Ala2, N-methyl-Phe4, Gly5-ol]enkephalin (DAMGO),
S, Kaneko +3 more
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Functional expression of opioid receptors was detected in the Xenopus oocyte translation system by a voltage-clamp recording. After injection of poly(A)+ RNA isolated from 3-week-old rat striatum or whole brain, the oocytes often demonstrated intracellular Ca(2+)-mediated oscillatory responsiveness to [D-Ala2, N-methyl-Phe4, Gly5-ol]enkephalin (DAMGO),
S, Kaneko +3 more
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Shock, 2002
There has been a widespread impression that tumor necrosis factor-alpha (TNF-alpha) and interleukin-1beta (IL-1beta) mediate the toxicity of high doses of lipopolysaccharide (LPS, endotoxin) and are key factors in septic shock. However, the clinical efficacy of treatment with antagonists of TNF-alpha and IL-1beta is still controversial, suggesting that
Wen-Hui, Fang +6 more
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There has been a widespread impression that tumor necrosis factor-alpha (TNF-alpha) and interleukin-1beta (IL-1beta) mediate the toxicity of high doses of lipopolysaccharide (LPS, endotoxin) and are key factors in septic shock. However, the clinical efficacy of treatment with antagonists of TNF-alpha and IL-1beta is still controversial, suggesting that
Wen-Hui, Fang +6 more
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Investigating the Effect of 5’ and 3’ UTRs on mRNA Mobility in Plants
In tomato rootstock and Nicotiana benthamiana heterografting systems, N. benthamiana scions show stress and defense-related transcripts derived from tomato rootstock. tRNA-like and poly-pyrimidine sequences in mRNA untranslated regions (UTRs) may facilitate mRNA mobility.Lin, Judith, Koretsky, Michael
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