Results 101 to 110 of about 308,109 (309)
Rapid screening of staphylokinase protein variants using an unpurified cell‐free expression system
FEBS Open Bio, EarlyView.An unpurified cell‐free protein synthesis (CFPS) platform enables rapid functional screening of staphylokinase variants. Direct plasminogen‐activation assays performed in microplate format provide real‐time activity readouts, allowing rapid identification and ranking of variants with improved or reduced fibrinolytic activity without protein ...
Maria Tomková +3 more
wiley +1 more source
Projection filters for modal parameter estimate for flexible structures [PDF]
Single-mode projection filters are developed for eigensystem parameter estimates from both analytical results and test data. Explicit formulations of these projection filters are derived using the pseudoinverse matrices of the controllability and ...
Chen, Chung-Wen, Huang, Jen-Kuang
core +1 more source
Systemic dysregulation of apolipoproteins in amyotrophic lateral sclerosis serum
FEBS Open Bio, EarlyView.Amyotrophic lateral sclerosis (ALS) is a fatal disease that damages motor neurons. This study found that people with ALS show significant changes in blood fats and the proteins that carry them. Several apolipoproteins were higher, lipid balances were altered, and normal protein–lipid relationships were disrupted.
Finula I. Isik +6 more
wiley +1 more source
Modal Parameter Identification from Output Data Only: Equivalent Approaches
Shock and Vibration, 2015 The problem of modal parameter identification from output data only is presented. To identify the modal parameters different algorithms are presented: the block Hankel matrix and its shifted version and the block observability and block controllability ...
Joseph Lardies
doaj +1 more source
Experimental Validation of Modal Parameters in Rotating Machinery [PDF]
In this paper, instability of rotating machinery systems due to rotating damping is investigated through experimental modal analysis. Generally, advanced methods are needed for these kind of systems because of the asymmetry of the matrices. However, it is shown by means of cyclic energy dissipation that the rotating damping can be handled as damping ...
Vervisch, Bram +2 more
openaire +2 more sources
FEBS Open Bio, EarlyView.Evolutionary analysis across 32 placental mammals identified positive selection at residues H148 and W149 in the immune receptor FcγR1. Ancestral reconstruction combined with molecular dynamics simulations reveals how these mutations may influence receptor structure and dynamics, providing insight into the evolution of antibody recognition and immune ...
David A. Young +7 more
wiley +1 more source
Algorithms for on-line parameter and mode shape estimation [PDF]
Algorithms are presented for on-line parameter and mode-shape estimation. The approach used is based upon a modal decomposition of the dynamic response of the flexible structure and is designed to make use of the parallel processing features of modern ...
Thau, F. E.
core +1 more source
Optimizing photoactivation of PA‐mCherry for optical pooled CRISPR screens
FEBS Open Bio, EarlyView.Photoactivatable PA‐mCherry finds widespread use to optically tag individual cells. However, confocal 405 nm UV laser‐scanning (normal scan) is much less efficient than widefield UV illumination, limiting the use of PA‐mCherry on confocal instruments. We remedy this limitation by reporting that rapid and repeated confocal scanning with a low‐intensity,
Sravasti Mukherjee +3 more
wiley +1 more source
FEBS Open Bio, EarlyView.Abruptly changing from aerobic to anaerobic conditions (sudden anaerobization) induced growth inhibition and a significant increase in intracellular labile ferrous iron in the aerotolerant anaerobe Amphibacillus xylanus. We found that free flavins mediate efficient electron transfer from NADH to ferric iron under anaerobic conditions, suggesting that ...
Shinya Kimata +13 more
wiley +1 more source
A New Maximum Likelihood Estimator Formulated in Pole-Residue Modal Model
Applied Sciences, 2019 Recently, a lot of efforts have been devoted to developing more precise Modal Parameter Estimation (MPE) techniques. This is explained by the necessity in civil, mechanical and aerospace engineering of obtaining accurate estimates for the modal ...
Sandro Amador +3 more
doaj +1 more source