Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies
FEBS Open Bio, EarlyView.We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli +8 more
wiley +1 more source
FEBS Open Bio, EarlyView.This study investigated a novel WST‐8‐based assay for evaluating d‐Amino acid oxidase (DAO) inhibitors. We confirmed its effectiveness using known inhibitors and found that uremic toxins possess relatively weak inhibitory activity compared to existing drugs.
Kahoko Miyake +4 more
wiley +1 more source
Study on Damage Detection of Cantilever Structure Based on Point Spectral Correlation Function
工程科学与技术, 2013 :In order to identify the damage location and extent of the cantilever structure, a flexible damage identification method was proposed based on point spectral correlation function.
doaj
Modal parameter identification based on NEXT/ERA time-domain joint algorithm
上海师范大学学报. 自然科学版, 2015 This paper summarizes the basic idea of NEXT algorithm and features of the system, and the basic steps of implementation.On this basis,a modal identification program is written using Matlab.The monitoring data of East China Sea bridge are selected as an ...
WANG Hongwei
doaj +1 more source
Output-Only Time-Varying Modal Parameter Identification Method Based on the TARMAX Model for the Milling of a Thin-Walled Workpiece. [PDF]
Micromachines (Basel), 2022 Ma J, Yan X, Li Y, Li H, Li Y, Pang X.
europepmc +1 more source
FEBS Open Bio, EarlyView.This protocol paper outlines methods to establish the success of a time‐resolved serial crystallographic experiment, by means of statistical analysis of timepoint data in reciprocal space and models in real space. We show how to amplify the signal from excited states to visualise structural changes in successful experiments.
Jake Hill +4 more
wiley +1 more source
Experimental modal analysis in research
Budownictwo i Architektura, 2017 Experimental modal analysis has grown steadily in popularity since the advent of the digital FFT spectrum analyser in the 1970’s. This days impact testing has become widespread as a fast and economical means of finding the vibration modes of a machine or
Mariusz Żółtowski +1 more
doaj +1 more source
Comparisons of Differential Filtering and Homography Transformation in Modal Parameter Identification from UAV Measurement. [PDF]
Sensors (Basel), 2021 Zhang J, Wu Z, Chen G, Liang Q.
europepmc +1 more source
Rapid screening of staphylokinase protein variants using an unpurified cell‐free expression system
FEBS Open Bio, EarlyView.An unpurified cell‐free protein synthesis (CFPS) platform enables rapid functional screening of staphylokinase variants. Direct plasminogen‐activation assays performed in microplate format provide real‐time activity readouts, allowing rapid identification and ranking of variants with improved or reduced fibrinolytic activity without protein ...
Maria Tomková +3 more
wiley +1 more source
High‐Fidelity Finite Element Model Development for Damage Detection of Wind Turbine Composite Blades
Wind EnergyThe structural stiffness of a blade changes when it is defective, which affects its modal parameters. Consequently, modal parameters can be crucial to investigating defects.
Sungmok Hwang +3 more
doaj +1 more source