Results 241 to 250 of about 168,707,534 (305)

Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies

FEBS Open Bio, EarlyView.
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli, Mohammad Nour Alsamsam, Ugnė Bagdonaitė, Van Truc Vu, Chun‐Jen Huang, Polina Kuzhir, Mindaugas Zaremba, Aurimas Kopūstas, Marijonas Tutkus   +8 more
wiley   +1 more source

Mode of action-specific and cause-specific retention of biologic and targeted synthetic disease-modifying antirheumatic drugs in anti-SS-A antibody-positive rheumatoid arthritis: The ANSWER cohort study. [PDF]

PLoS One
Nishisaka K, Okano T, Imai T, Tsubosaka M, Kamenaga T, Nakano N, Hayashi S, Yamamoto W, Onishi A, Murakami K, Tsujimoto K, Katsushima M, Yoshikawa A, Kotani T, Amuro H, Son Y, Itami T, Nozaki Y, Nose Y, Yamashita M, Shirasugi I, Yamada H, Nishimura K, Ueda Y, Sendo S, Hashimoto M, Kuroda R, Saegusa J.   +27 more
europepmc   +1 more source

Optimizing photoactivation of PA‐mCherry for optical pooled CRISPR screens

FEBS Open Bio, EarlyView.
Photoactivatable PA‐mCherry finds widespread use to optically tag individual cells. However, confocal 405 nm UV laser‐scanning (normal scan) is much less efficient than widefield UV illumination, limiting the use of PA‐mCherry on confocal instruments. We remedy this limitation by reporting that rapid and repeated confocal scanning with a low‐intensity,
Sravasti Mukherjee, Giulia Zanetti, Bram van den Broek, Kees Jalink   +3 more
wiley   +1 more source

Comparative Analysis of Plant Defense Activation by Four Biosurfactants: Mode of Action and Disease Control Potential. [PDF]

Int J Mol Sci
Aoki Y, Asada T, Nojiri M, Suzuki S.
europepmc   +1 more source

PreMode predicts mode-of-action of missense variants by deep graph representation learning of protein sequence and structural context. [PDF]

Nat Commun
Zhong G, Zhao Y, Zhuang D, Chung WK, Shen Y.   +4 more
europepmc   +1 more source

Anti?atherosclerotic effect of aged garlic extract: Mode of action and therapeutic benefits (Review). [PDF]

Exp Ther Med
Miki S, Takashima M, Suzuki JI.
europepmc   +1 more source

Knockout of nAChR subunits in spider mites and their phytoseiid predators confers spinosyn cross-resistance and reveals a conserved mode of action in mites.

Insect Biochem Mol Biol
Mocchetti A, Steelant P, Hosseinkhani M, De Rouck S, Khajehali J, Van Leeuwen T.   +5 more
europepmc   +1 more source

Antitubercular potential and pH-driven mode of action of salicylic acid derivatives. [PDF]

FEBS Open Bio
Laudouze J, Francis T, Forest E, Mies F, Bolla JM, Crauste C, Canaan S, Shlyonsky V, Santucci P, Cavalier JF.   +9 more
europepmc   +1 more source

Unprecedented Photoinduced-Electron-Transfer Probe with a Turn-ON Chemiluminescence Mode-of-Action. [PDF]

Angew Chem Int Ed Engl
David M, Gutkin S, Nithun RV, Jbara M, Shabat D.   +4 more
europepmc   +1 more source