Versatile vector tools for efficient protein screening across multiple expression systems
A unified vector toolkit enables rapid protein expression screening across E. coli, insect, and mammalian cells. A single primer pair amplifies the target gene, which is inserted into any vector via a standardized interface. This streamlined workflow eliminates repeated cloning steps, accelerating the identification of optimal expression conditions for
Zhimin Zhu +5 more
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Sensor-Based Tracking and Localization of In-Line Inspection Tools in Oil and Gas Pipelines: A Review. [PDF]
Zheng J, Ju B, Sun A.
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Time‐restricted feeding (TRF) in mice increased liver fatty acid oxidation and decreased fatty acid biosynthesis. These alterations persisted when TRF was discontinued and the host was infected with Mycobacterium tuberculosis. Pre‐exposure to TRF did not alter tissue (lung and spleen) mycobacterial burden but significantly reduced CD3+ T cells in lungs
Ashish Gupta +7 more
wiley +1 more source
Quantitative Characterization of Flow Hysteresis for Fractured-Vuggy Underground Gas Storage during High-Rate Injection and Production Based on Multiscale Digital Cores. [PDF]
Li L, Wang M, Zhang L, Wang R, Yu P.
europepmc +1 more source
In a murine model of myocardial ischemia and reperfusion (MI/R), the CD36 azapeptide ligand MPE‐298 reduces cardiac injury and transiently lowers left ventricular long‐chain fatty acids (LCFAs) accumulation 3 h after reperfusion, accompanied by a decrease of oxidative stress and inflammation‐associated genes' expression in the heart and adipose tissue.
Jade Gauvin +12 more
wiley +1 more source
AI-driven predictive modelling of residual stress of HVOF thermal sprayed carbon-based composite coatings using physics-informed neural networks. [PDF]
Tyagi A, Dadhich A, Sirohi S, Gupta AK.
europepmc +1 more source
UiO‐66(Zr) metal–organic frameworks are chemically stable, biocompatible, and highly tunable nanomaterials. Their modular structure enables controlled drug delivery, multimodal bioimaging, and light‐activated photodynamic therapy, supporting integrated diagnostic and therapeutic (theranostic) applications in cancer and biomedical research.
Veronika Huntošová +2 more
wiley +1 more source
A Distance-Adaptive Method for Three-Axis Angle Measurement Based on an Optical Wedge. [PDF]
Wang J, Ran C, Wang L, Zhang L, Bai Y.
europepmc +1 more source
Directed evolution of enzymes at the crossroads of tradition and innovation
An iterative cycle of data‐driven enzyme optimization comprising four stages: genetic diversification of a template enzyme, expression of protein variants, high‐throughput evaluation, and machine‐learning‐guided redesign of the next variant library.
Maria Tomkova +2 more
wiley +1 more source
Vibration response of blue honeysuckle branches based on mathematical model. [PDF]
Wei Y +4 more
europepmc +1 more source

