Purification tags markedly affect self‐aggregation of CPEB3
Although recombinant proteins are used to study protein aggregation in vitro, uncleaved tags can interfere with accurate interpretation. Our findings demonstrate that His₆‐GFP and His₁₂ tags significantly affect liquid droplet and amyloid fibril formation in the intrinsically disordered region (IDR) of mouse cytoplasmic polyadenylation element‐binding ...
Harunobu Saito+6 more
wiley +1 more source
Non-linear properties of supercooled liquids in the system Na2O---SiO2 [PDF]
The physical properties, viscosity, density, heat capacity and thermal expansivity, of relaxed supercooled liquids in the temperature range just above the glass transition have been determined for ten compositions along the compositional binary Na2O ...
Dingwell, Donald B.+3 more
core +1 more source
In this work, we reveal how different enzyme binding configurations influence the fluorescence decay of NAD(P)H in live cells using time‐resolved anisotropy imaging and fluorescence lifetime imaging microscopy (FLIM). Mathematical modelling shows that the redox states of the NAD and NADP pools govern these configurations, shaping their fluorescence ...
Thomas S. Blacker+8 more
wiley +1 more source
Cofinement, entropy, and single-particle dynamics of equilibrium hard-sphere mixtures
We use discontinuous molecular dynamics and grand-canonical transition-matrix Monte Carlo simulations to explore how confinement between parallel hard walls modifies the relationships between packing fraction, self-diffusivity, partial molar excess ...
Davis H. T.+6 more
core +1 more source
Molar mass and solution conformation of branched alpha(1 - 4), alpha(1 - 6) Glucans. Part I: Glycogens in water [PDF]
Solution molar masses and conformations of glycogens from different sources (rabbit, oyster, mussel and bovine) were analysed using sedimentation velocity in the analytical ultracentrifuge, size-exclusion chromatography coupled to multi-angle laser light
Ang, S.+6 more
core +1 more source
Microbial exopolysaccharide production by polyextremophiles in the adaptation to multiple extremes
Polyextremophiles are microorganisms that endure multiple extreme conditions by various adaptation strategies that also include the production of exopolysaccharides (EPSs). This review provides an integrated perspective on EPS biosynthesis, function, and regulation in these organisms, emphasizing their critical role in survival and highlighting their ...
Tracey M Gloster, Ebru Toksoy Öner
wiley +1 more source
Evaluation of First Permanent Molar Extractions Due to Caries in Children
Objective: The aim of this study was to determine the frequency of the first permanent molars extracted due to caries in children and their pre-extraction treatment status.
Müge TOKUÇ, Merve ÇELİK
doaj +1 more source
A nucleotide‐independent, pan‐RAS‐targeted DARPin elicits anti‐tumor activity in a multimodal manner
We report a Designed Ankyrin Repeat Protein that binds and inhibits RAS proteins, which serve as central cell signaling hubs and are essential for the progression of many cancers. Its unique feature is that it does not discriminate between different RAS isoforms or mutations and is capable of binding to RAS in both its active (GTP‐bound) and inactive ...
Jonas N. Kapp+13 more
wiley +1 more source
Comparison of two systems of tooth numbering among undergraduate dental students
Background: Dental charting is the basis of treatment in dentistry. It should be recorded to know the presence of healthy or diseased, for communication purpose with the colleagues and also used for reference purpose.
Deepthi Kannan, Deepa Gurunathan
doaj +1 more source
Removal of an aspirated tooth from the bronchus using a cryoprobe: A case report
An 88‐year‐old bedridden man with Alzheimer's disease developed fever and hypoxaemia. Chest radiography showed obstructive pneumonia caused by a foreign body in the airway.
Hiroshi Ishimoto+11 more
doaj +1 more source