Results 51 to 60 of about 75,375 (253)
FEBS Open Bio, EarlyView.Unique biological samples, such as site‐specific mutant proteins, are available only in limited quantities. Here, we present a polarization‐resolved transient infrared spectroscopy setup with referencing to improve signal‐to‐noise tailored towards tracing small signals. We provide an overview of characterizing the excitation conditions for polarization‐
Clark Zahn, Karsten Heyne
wiley +1 more source
FEBS Open Bio, EarlyView.Time‐resolved X‐ray solution scattering captures how proteins change shape in real time under near‐native conditions. This article presents a practical workflow for light‐triggered TR‐XSS experiments, from data collection to structural refinement. Using a calcium‐transporting membrane protein as an example, the approach can be broadly applied to study ...
Fatemeh Sabzian‐Molaei +3 more
wiley +1 more source
Spin-orbital-angular-momentum-coupled quantum gases
AAPPS Bulletin, 2022 We briefly review the recent progress of theories and experiments on spin-orbital-angular-momentum (SOAM)-coupled quantum gases. The coupling between the intrinsic degree of freedom of particles and their external orbital motions widely exists in the ...
Shi-Guo Peng +5 more
doaj +1 more source
Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies
FEBS Open Bio, EarlyView.We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli +8 more
wiley +1 more source
Supervised learning of time-independent Hamiltonians for gate design
New Journal of Physics, 2020 We present a general framework to tackle the problem of finding time-independent dynamics generating target unitary evolutions. We show that this problem is equivalently stated as a set of conditions over the spectrum of the time-independent gate ...
Luca Innocenti +4 more
doaj +1 more source
FEBS Open Bio, EarlyView.This protocol paper outlines methods to establish the success of a time‐resolved serial crystallographic experiment, by means of statistical analysis of timepoint data in reciprocal space and models in real space. We show how to amplify the signal from excited states to visualise structural changes in successful experiments.
Jake Hill +4 more
wiley +1 more source
Enhancing non-classicality in mechanical systems
New Journal of Physics, 2013 We study the effects of post-selection measurements on both the non-classicality of the state of a mechanical oscillator and the entanglement between two mechanical systems that are part of a distributed optomechanical network.
Jie Li +2 more
doaj +1 more source
Directed evolution of enzymes at the crossroads of tradition and innovation
FEBS Open Bio, EarlyView.An iterative cycle of data‐driven enzyme optimization comprising four stages: genetic diversification of a template enzyme, expression of protein variants, high‐throughput evaluation, and machine‐learning‐guided redesign of the next variant library.
Maria Tomkova +2 more
wiley +1 more source
Phase-dependent transparency in a two-level system with applications to all-optical switching
Frontiers in Quantum Science and TechnologyThe phenomenon of transparency, conventionally studied in three and higher level atomic systems, is extended to the case of a two-level system (TLS), where we use a semiclassical framework to describe the transparent propagation of classical fields in a ...
Aneesh Ramaswamy +1 more
doaj +1 more source
Implications of non-Markovian quantum dynamics for the Landauer bound
New Journal of Physics, 2016 We study the dynamics of a spin-1/2 particle interacting with a multi-spin environment, modelling the corresponding open system dynamics through a collision-based model.
Marco Pezzutto +2 more
doaj +1 more source