Results 31 to 40 of about 35,830 (304)
CRISPRI‐mediated gene silencing and phenotypic exploration in nontuberculous mycobacteria. In this Research Protocol, we describe approaches to control, monitor, and quantitatively assess CRISPRI‐mediated gene silencing in M. smegmatis and M. abscessus model organisms.
Vanessa Point +7 more
wiley +1 more source
Continuous thermochemical sources of AlF molecules
The AlF molecule, currently subject to laser cooling and trapping efforts, has the advantage that it can be efficiently produced in a thermochemical reaction between sublimated aluminum trifluoride and aluminum metal.
Pulkit Kukreja +12 more
doaj +1 more source
Unique biological samples, such as site‐specific mutant proteins, are available only in limited quantities. Here, we present a polarization‐resolved transient infrared spectroscopy setup with referencing to improve signal‐to‐noise tailored towards tracing small signals. We provide an overview of characterizing the excitation conditions for polarization‐
Clark Zahn, Karsten Heyne
wiley +1 more source
Laser-Cooled Ion Beams and Strongly Coupled Plasmas for Precision Experiments [PDF]
The first part of this thesis summarizes the results of laser-cooling of relativistic C3+ ion beams at the ESR/GSI. It is shown that laser cooling at high beam energies is feasible and that momentum spreads much smaller than those observed for electron ...
Bussmann, Michael
core
Time‐resolved X‐ray solution scattering captures how proteins change shape in real time under near‐native conditions. This article presents a practical workflow for light‐triggered TR‐XSS experiments, from data collection to structural refinement. Using a calcium‐transporting membrane protein as an example, the approach can be broadly applied to study ...
Fatemeh Sabzian‐Molaei +3 more
wiley +1 more source
Anomaly in H2+ Induced X-Ray Emission: An Experimental approach [PDF]
Interaction of energetic ion beam with matter results in atomic excitation and ionization of matter which consequently leads to occurance of the characteristic X-rays fluorescence.
V Keyvan, O.R Kakuee, F Shokouhi
doaj
Controlling molecular broadband-emission by optical confinement [PDF]
We investigate experimentally and theoretically the fluorescence emitted by molecular ensembles as well as spatially isolated, single molecules of an organic dye immobilized in a quasi-planar optical microresonator at room temperature.
Steiner, Mathias +5 more
core +1 more source
This protocol paper outlines methods to establish the success of a time‐resolved serial crystallographic experiment, by means of statistical analysis of timepoint data in reciprocal space and models in real space. We show how to amplify the signal from excited states to visualise structural changes in successful experiments.
Jake Hill +4 more
wiley +1 more source
A detailed account of the measurements of cold collisions in a molecular synchrotron
We have recently demonstrated a general and sensitive method to study low energy collisions that exploits the unique properties of a molecular synchrotron (Van der Poel et al., Phys Rev Lett 120:033402, 2018).
Aernout P. P. van der Poel +1 more
doaj +1 more source
Light-Induced Ring Pattern in a Dye-Doped Nematic Liquid Crystal
The use of dye-doped liquid crystals allows the amplification of the coupling of light and liquid crystals. Light can induce the self-organization of the molecular order.
Marcel G. Clerc +5 more
doaj +1 more source

