Results 31 to 40 of about 35,830 (304)

Mycobacterial cell division arrest and smooth‐to‐rough envelope transition using CRISPRi‐mediated genetic repression systems

open access: yesFEBS Open Bio, EarlyView.
CRISPRI‐mediated gene silencing and phenotypic exploration in nontuberculous mycobacteria. In this Research Protocol, we describe approaches to control, monitor, and quantitatively assess CRISPRI‐mediated gene silencing in M. smegmatis and M. abscessus model organisms.
Vanessa Point   +7 more
wiley   +1 more source

Continuous thermochemical sources of AlF molecules

open access: yesNew Journal of Physics
The AlF molecule, currently subject to laser cooling and trapping efforts, has the advantage that it can be efficiently produced in a thermochemical reaction between sublimated aluminum trifluoride and aluminum metal.
Pulkit Kukreja   +12 more
doaj   +1 more source

Polarization‐resolved femtosecond Vis/IR spectroscopy tailored for resolving weak signals in biological samples using minimal sample volume

open access: yesFEBS Open Bio, EarlyView.
Unique biological samples, such as site‐specific mutant proteins, are available only in limited quantities. Here, we present a polarization‐resolved transient infrared spectroscopy setup with referencing to improve signal‐to‐noise tailored towards tracing small signals. We provide an overview of characterizing the excitation conditions for polarization‐
Clark Zahn, Karsten Heyne
wiley   +1 more source

Laser-Cooled Ion Beams and Strongly Coupled Plasmas for Precision Experiments [PDF]

open access: yes, 2008
The first part of this thesis summarizes the results of laser-cooling of relativistic C3+ ion beams at the ESR/GSI. It is shown that laser cooling at high beam energies is feasible and that momentum spreads much smaller than those observed for electron ...
Bussmann, Michael
core  

A light‐triggered Time‐Resolved X‐ray Solution Scattering (TR‐XSS) workflow with application to protein conformational dynamics

open access: yesFEBS Open Bio, EarlyView.
Time‐resolved X‐ray solution scattering captures how proteins change shape in real time under near‐native conditions. This article presents a practical workflow for light‐triggered TR‐XSS experiments, from data collection to structural refinement. Using a calcium‐transporting membrane protein as an example, the approach can be broadly applied to study ...
Fatemeh Sabzian‐Molaei   +3 more
wiley   +1 more source

Anomaly in H2+ Induced X-Ray Emission: An Experimental approach [PDF]

open access: yesمجله علوم و فنون هسته‌ای, 2012
Interaction of energetic ion beam with matter results in atomic excitation and ionization of matter which consequently leads to occurance of the characteristic X-rays fluorescence.
V Keyvan, O.R Kakuee, F Shokouhi
doaj  

Controlling molecular broadband-emission by optical confinement [PDF]

open access: yes, 2008
We investigate experimentally and theoretically the fluorescence emitted by molecular ensembles as well as spatially isolated, single molecules of an organic dye immobilized in a quasi-planar optical microresonator at room temperature.
Steiner, Mathias   +5 more
core   +1 more source

Analysing the significance of small conformational changes and low occupancy states in serial crystallographic data

open access: yesFEBS Open Bio, EarlyView.
This protocol paper outlines methods to establish the success of a time‐resolved serial crystallographic experiment, by means of statistical analysis of timepoint data in reciprocal space and models in real space. We show how to amplify the signal from excited states to visualise structural changes in successful experiments.
Jake Hill   +4 more
wiley   +1 more source

A detailed account of the measurements of cold collisions in a molecular synchrotron

open access: yesEPJ Techniques and Instrumentation, 2018
We have recently demonstrated a general and sensitive method to study low energy collisions that exploits the unique properties of a molecular synchrotron (Van der Poel et al., Phys Rev Lett 120:033402, 2018).
Aernout P. P. van der Poel   +1 more
doaj   +1 more source

Light-Induced Ring Pattern in a Dye-Doped Nematic Liquid Crystal

open access: yesApplied Sciences, 2021
The use of dye-doped liquid crystals allows the amplification of the coupling of light and liquid crystals. Light can induce the self-organization of the molecular order.
Marcel G. Clerc   +5 more
doaj   +1 more source

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