Results 31 to 40 of about 25,457 (267)

microDuMIP: target-enrichment technique for microarray-based duplex molecular inversion probes [PDF]

Nucleic Acids Research, 2014
Molecular inversion probe (MIP)-based capture is a scalable and effective target-enrichment technology that can use synthetic single-stranded oligonucleotides as probes. Unlike the straightforward use of synthetic oligonucleotides for low-throughput target capture, high-throughput MIP capture has required laborious protocols to generate thousands of ...
Jung-Ki Yoon, Jinwoo Ahn, Han Sang Kim, Soo Min Han, Hoon Jang, Min Goo Lee, Ji Hyun Lee, Duhee Bang   +7 more
openalex   +5 more sources

MON-472 Potentially Pathogenic Variants Identified in Patients with Hypopituitarism by Molecular Inversion Probe Sequencing (MIPS), a New Molecular Approach for Low Cost Gene Panel Sequencing

J Endocr Soc, 2019
Vishnopolska S, Mortensen A, Braslavsky D, Keselman A, Bergada I, Marino R, Ramirez P, Perez Garrido N, Ciaccio M, Di Palma M, Belgorosky A, Marti M, Kitzman J, Camper S, Perez Millan M.   +14 more
europepmc   +2 more sources

An improved molecular inversion probe based targeted sequencing approach for low variant allele frequency. [PDF]

NAR Genom Bioinform, 2022
AbstractDeep targeted sequencing technologies are still not widely used in clinical practice due to the complexity of the methods and their cost. The Molecular Inversion Probes (MIP) technology is cost effective and scalable in the number of targets, however, suffers from low overall performance especially in GC rich regions.
Biezuner T, Brilon Y, Arye AB, Oron B, Kadam A, Danin A, Furer N, Minden MD, Hwan Kim DD, Shapira S, Arber N, Dick J, Thavendiranathan P, Moskovitz Y, Kaushansky N, Chapal-Ilani N, Shlush LI.   +16 more
europepmc   +4 more sources

Genome-wide copy number aberrations and HER2 and FGFR1 alterations in primary breast cancer by molecular inversion probe microarray. [PDF]

Oncotarget, 2017
Chen H, Singh RR, Lu X, Huo L, Yao H, Aldape K, Abraham R, Virani S, Mehrotra M, Mishra BM, Bousamra A, Albarracin C, Wu Y, Roy-Chowdhuri S, Kanagal-Shamanna R, Routbort MJ, Medeiros LJ, Patel KP, Broaddus R, Sahin A, Luthra R.   +20 more
europepmc   +3 more sources

SNP typing using molecular inversion probes [PDF]

Forensic Science International: Genetics Supplement Series, 2008
Single nucleotide polymorphism (SNP) typing will be useful in forensic analysis but is limited by the multiplexing capacity of multiple targets in the PCR. A strategy to circumvent this problem can be to use molecular inversion probes (MIPs). MIPs are linear oligonucleotides 70–100 nt in length, with end-segments that are complementary to a target ...
Hanna Edlund, Marie Allen
openalex   +3 more sources

Aligning sequence from molecular inversion probes [PDF]

, 2014
ABSTRACTSummaryMolecular inversion probes (MIP’s) allow efficient enrichment of genomic regions of interest for the purpose of targeted sequencing. To date, there is a paucity of simple-to-use software to align sequences derived from this method. Here, we describe a single program that performs mapping, arm removal, and deduplication before outputting ...
Brent S. Pedersen, Elissa Murphy, Ivana V. Yang, David A. Schwartz   +3 more
openalex   +2 more sources

Exome Survey and Candidate Gene Re-Sequencing Identifies Novel Exstrophy Candidate Genes and Implicates LZTR1 in Disease Formation

Biomolecules, 2023
Background: The bladder exstrophy-epispadias complex (BEEC) is a spectrum of congenital abnormalities that involves the abdominal wall, the bony pelvis, the urinary tract, the external genitalia, and, in severe cases, the gastrointestinal tract as well ...
Ricarda Köllges, Jil Stegmann, Sophia Schneider, Lea Waffenschmidt, Julia Fazaal, Katinka Breuer, Alina C. Hilger, Gabriel C. Dworschak, Enrico Mingardo, Wolfgang Rösch, Aybike Hofmann, Claudia Neissner, Anne-Karolin Ebert, Raimund Stein, Nina Younsi, Karin Hirsch-Koch, Eberhard Schmiedeke, Nadine Zwink, Ekkehart Jenetzky, Holger Thiele, Kerstin U. Ludwig, Heiko Reutter   +21 more
doaj   +1 more source

MIPgen: optimized modeling and design of molecular inversion probes for targeted resequencing [PDF]

Bioinformatics, 2014
Abstract Summary Molecular inversion probes (MIPs) enable cost-effective multiplex targeted gene resequencing in large cohorts. However, the design of individual MIPs is a critical parameter governing the performance of this technology with respect to capture uniformity and specificity.
Evan A. Boyle, Brian J. O’Roak, Beth Martin, Akash Kumar, Jay Shendure   +4 more
openalex   +4 more sources

PhenoMIP: High-Throughput Phenotyping of Diverse Caenorhabditis elegans Populations via Molecular Inversion Probes [PDF]

G3 Genes|Genomes|Genetics, 2020
Abstract Whether generated within a lab setting or isolated from the wild, variant alleles continue to be an important resource for decoding gene function in model organisms such as Caenorhabditis elegans. With advances in massively parallel sequencing, multiple whole-genome sequenced (WGS) strain collections are now available to the ...
Calvin Mok, Gabriella Belmarez, Mark L. Edgley, Donald G. Moerman, R Waterston   +4 more
openalex   +5 more sources

Using population selection and sequencing to characterize natural variation of starvation resistance in Caenorhabditis elegans

eLife, 2022
Starvation resistance is important to disease and fitness, but the genetic basis of its natural variation is unknown. Uncovering the genetic basis of complex, quantitative traits such as starvation resistance is technically challenging.
Amy K Webster, Rojin Chitrakar, Maya Powell, Jingxian Chen, Kinsey Fisher, Robyn E Tanny, Lewis Stevens, Kathryn Evans, Angela Wei, Igor Antoshechkin, Erik C Andersen, L Ryan Baugh   +11 more
doaj   +1 more source