Results 41 to 50 of about 155,803 (258)

PhenoMIP: High-Throughput Phenotyping of Diverse Caenorhabditis elegans Populations via Molecular Inversion Probes [PDF]

G3 Genes|Genomes|Genetics, 2020
Abstract Whether generated within a lab setting or isolated from the wild, variant alleles continue to be an important resource for decoding gene function in model organisms such as Caenorhabditis elegans. With advances in massively parallel sequencing, multiple whole-genome sequenced (WGS) strain collections are now available to the ...
Calvin Mok, Gabriella Belmarez, Mark L. Edgley, Donald G. Moerman, R Waterston   +4 more
openalex   +5 more sources

Using population selection and sequencing to characterize natural variation of starvation resistance in Caenorhabditis elegans

eLife, 2022
Starvation resistance is important to disease and fitness, but the genetic basis of its natural variation is unknown. Uncovering the genetic basis of complex, quantitative traits such as starvation resistance is technically challenging.
Amy K Webster, Rojin Chitrakar, Maya Powell, Jingxian Chen, Kinsey Fisher, Robyn E Tanny, Lewis Stevens, Kathryn Evans, Angela Wei, Igor Antoshechkin, Erik C Andersen, L Ryan Baugh   +11 more
doaj   +1 more source

Connector inversion probe technology: a powerful one-primer multiplex DNA amplification system for numerous scientific applications. [PDF]

PLoS ONE, 2007
We combined components of a previous assay referred to as Molecular Inversion Probe (MIP) with a complete gap filling strategy, creating a versatile powerful one-primer multiplex amplification system.
Michael S Akhras, Magnus Unemo, Sreedevi Thiyagarajan, Pål Nyrén, Ronald W Davis, Andrew Z Fire, Nader Pourmand   +6 more
doaj   +1 more source

Molecular Inversion Probes for targeted resequencing in non-model organisms

Scientific Reports, 2016
AbstractApplications that require resequencing of hundreds or thousands of predefined genomic regions in numerous samples are common in studies of non-model organisms. However few approaches at the scale intermediate between multiplex PCR and sequence capture methods are available.
Marta Niedzicka, Anna Fijarczyk, Katarzyna Dudek, Michał Stuglik, Wiesław Babik   +4 more
openalex   +4 more sources

Targeted next-generation sequencing for the detection of ciprofloxacin resistance markers using molecular inversion probes [PDF]

Scientific Reports, 2016
AbstractAntibiotic resistance (AR) is an epidemic of increasing magnitude requiring rapid identification and profiling for appropriate and timely therapeutic measures and containment strategies. In this context, ciprofloxacin is part of the first-line of countermeasures against numerous high consequence bacteria.
Christopher P. Stefan, Jeffrey W. Koehler, Timothy D. Minogue   +2 more
openalex   +3 more sources

PathogenMip assay: a multiplex pathogen detection assay. [PDF]

PLoS ONE, 2007
The Molecular Inversion Probe (MIP) assay has been previously applied to a large-scale human SNP detection. Here we describe the PathogenMip Assay, a complete protocol for probe production and applied approaches to pathogen detection.
Michael S Akhras, Sreedevi Thiyagarajan, Andrea C Villablanca, Ronald W Davis, Pål Nyrén, Nader Pourmand   +5 more
doaj   +1 more source

Development of Molecular Inversion Probes for Soybean Progeny Genomic Selection Genotyping

The Plant Genome, 2022
AbstractIncreasing rate of genetic gain for key agronomic traits through genomic selection requires the development of new molecular methods to run genome-wide single nucleotide polymorphisms (SNPs). The main limitation of current methods is the cost is too high to screen breeding populations.
Haichuan Wang, Benjamin Campbell, Mary Happ, Samantha McConaughy, Aaron Lorenz, Keenan Amundsen, Qijian Song, Vincent Pantalone, David Hyten   +8 more
openaire   +3 more sources

Ultrasensitive detection of acute myeloid leukemia minimal residual disease using single molecule molecular inversion probes

Haematologica, 2017
The identification of minimal residual disease is the primary diagnostic finding which predicts relapse in patients treated for acute myeloid leukemia. Ultrasensitive detection of minimal residual disease would enable better patient risk stratification ...
Adam Waalkes, Kelsi Penewit, Brent L. Wood, David Wu, Stephen J. Salipante   +4 more
doaj   +1 more source

Inverse Mixed-Solvent Molecular Dynamics for Visualization of the Residue Interaction Profile of Molecular Probes

International Journal of Molecular Sciences, 2022
To ensure efficiency in discovery and development, the application of computational technology is essential. Although virtual screening techniques are widely applied in the early stages of drug discovery research, the computational methods used in lead optimization to improve activity and reduce the toxicity of compounds are still evolving.
Keisuke Yanagisawa, Ryunosuke Yoshino, Genki Kudo, Takatsugu Hirokawa   +3 more
openaire   +2 more sources

BRCA Testing by Single-Molecule Molecular Inversion Probes

Clinical Chemistry, 2017
Abstract BACKGROUND Despite advances in next generation DNA sequencing (NGS), NGS-based single gene tests for diagnostic purposes require improvements in terms of completeness, quality, speed, and cost.
Neveling, K., Mensenkamp, A.R., Derks, R, Kwint, M.P., Ouchene, H., Steehouwer, M., van Lier, L.A., Bosgoed, E.A.J., Rikken, A., Tychon, M.W.J., Zafeiropoulou, D., Castelein, S., Hehir-Kwa, J.Y., Thung, G.W., Hofste, T., Lelieveld, S.H., Bertens, S.M., Adan, I.B., Eijkelenboom, A., Tops, B.B.J., Yntema, H.G., Stokowy, T., Knappskog, P.M., Hoberg-Vetti, H., Steen, V.M., Boyle, E., Martin, B., Ligtenberg, M.J.L., Ligtenberg, M.J.L., Shendure, J., Nelen, M.R., Hoischen, A., Hoischen, A.   +32 more
openaire   +4 more sources