Results 41 to 50 of about 25,457 (267)

Connector inversion probe technology: a powerful one-primer multiplex DNA amplification system for numerous scientific applications. [PDF]

PLoS ONE, 2007
We combined components of a previous assay referred to as Molecular Inversion Probe (MIP) with a complete gap filling strategy, creating a versatile powerful one-primer multiplex amplification system.
Michael S Akhras, Magnus Unemo, Sreedevi Thiyagarajan, Pål Nyrén, Ronald W Davis, Andrew Z Fire, Nader Pourmand   +6 more
doaj   +1 more source

BRCA Testing by Single-Molecule Molecular Inversion Probes [PDF]

Clinical Chemistry, 2017
Abstract BACKGROUND Despite advances in next generation DNA sequencing (NGS), NGS-based single gene tests for diagnostic purposes require improvements in terms of completeness, quality, speed, and cost.
Neveling, K., Mensenkamp, A.R., Derks, R, Kwint, M.P., Ouchene, H., Steehouwer, M., van Lier, L.A., Bosgoed, E.A.J., Rikken, A., Tychon, M.W.J., Zafeiropoulou, D., Castelein, S., Hehir-Kwa, J.Y., Thung, G.W., Hofste, T., Lelieveld, S.H., Bertens, S.M., Adan, I.B., Eijkelenboom, A., Tops, B.B.J., Yntema, H.G., Stokowy, T., Knappskog, P.M., Hoberg-Vetti, H., Steen, V.M., Boyle, E., Martin, B., Ligtenberg, M.J.L., Ligtenberg, M.J.L., Shendure, J., Nelen, M.R., Hoischen, A., Hoischen, A.   +32 more
openaire   +3 more sources

Molecular Inversion Probes for targeted resequencing in non-model organisms

Scientific Reports, 2016
AbstractApplications that require resequencing of hundreds or thousands of predefined genomic regions in numerous samples are common in studies of non-model organisms. However few approaches at the scale intermediate between multiplex PCR and sequence capture methods are available.
Marta Niedzicka, Anna Fijarczyk, Katarzyna Dudek, Michał Stuglik, Wiesław Babik   +4 more
openalex   +4 more sources

Targeted next-generation sequencing for the detection of ciprofloxacin resistance markers using molecular inversion probes [PDF]

Scientific Reports, 2016
AbstractAntibiotic resistance (AR) is an epidemic of increasing magnitude requiring rapid identification and profiling for appropriate and timely therapeutic measures and containment strategies. In this context, ciprofloxacin is part of the first-line of countermeasures against numerous high consequence bacteria.
Christopher P. Stefan, Jeffrey W. Koehler, Timothy D. Minogue   +2 more
openalex   +3 more sources

Molecular Inversion Probe Analysis of Gene Copy Alterations Reveals Distinct Categories of Colorectal Carcinoma [PDF]

Cancer Research, 2006
Abstract Genomic instability is a major feature of neoplastic development in colorectal carcinoma and other cancers. Specific genomic instability events, such as deletions in chromosomes and other alterations in gene copy number, have potential utility as biologically relevant prognostic biomarkers.
Hanlee P. Ji, Jochen Kumm, Michael Zhang, Kyle Farnam, Keyan Salari, Malek Faham, James M. Ford, Ronald W. Davis   +7 more
openalex   +5 more sources

PathogenMip assay: a multiplex pathogen detection assay. [PDF]

PLoS ONE, 2007
The Molecular Inversion Probe (MIP) assay has been previously applied to a large-scale human SNP detection. Here we describe the PathogenMip Assay, a complete protocol for probe production and applied approaches to pathogen detection.
Michael S Akhras, Sreedevi Thiyagarajan, Andrea C Villablanca, Ronald W Davis, Pål Nyrén, Nader Pourmand   +5 more
doaj   +1 more source

Development of Molecular Inversion Probes for Soybean Progeny Genomic Selection Genotyping

The Plant Genome, 2022
AbstractIncreasing rate of genetic gain for key agronomic traits through genomic selection requires the development of new molecular methods to run genome-wide single nucleotide polymorphisms (SNPs). The main limitation of current methods is the cost is too high to screen breeding populations.
Haichuan Wang, Benjamin Campbell, Mary Happ, Samantha McConaughy, Aaron Lorenz, Keenan Amundsen, Qijian Song, Vincent Pantalone, David Hyten   +8 more
openaire   +3 more sources

A Genetics-First Approach Revealed Monogenic Disorders in Patients With ARM and VACTERL Anomalies

Frontiers in Pediatrics, 2020
Background: The VATER/VACTERL association (VACTERL) is defined as the non-random occurrence of the following congenital anomalies: Vertebral, Anal, Cardiac, Tracheal-Esophageal, Renal, and Limb anomalies.
Romy van de Putte, Gabriel C. Dworschak, Gabriel C. Dworschak, Erwin Brosens, Erwin Brosens, Heiko M. Reutter, Heiko M. Reutter, Carlo L. M. Marcelis, Rocio Acuna-Hidalgo, Nehir E. Kurtas, Marloes Steehouwer, Sally L. Dunwoodie, Eberhard Schmiedeke, Stefanie Märzheuser, Nicole Schwarzer, Alice S. Brooks, Annelies de Klein, Cornelius E. J. Sloots, Dick Tibboel, Giulia Brisighelli, Giulia Brisighelli, Anna Morandi, Maria F. Bedeschi, Michael D. Bates, Michael D. Bates, Marc A. Levitt, Marc A. Levitt, Marc A. Levitt, Alberto Peña, Alberto Peña, Alberto Peña, Ivo de Blaauw, Nel Roeleveld, Han G. Brunner, Han G. Brunner, Iris A. L. M. van Rooij, Alexander Hoischen, Alexander Hoischen   +37 more
doaj   +1 more source

Inverse Mixed-Solvent Molecular Dynamics for Visualization of the Residue Interaction Profile of Molecular Probes

International Journal of Molecular Sciences, 2022
To ensure efficiency in discovery and development, the application of computational technology is essential. Although virtual screening techniques are widely applied in the early stages of drug discovery research, the computational methods used in lead optimization to improve activity and reduce the toxicity of compounds are still evolving.
Keisuke Yanagisawa, Ryunosuke Yoshino, Genki Kudo, Takatsugu Hirokawa   +3 more
openaire   +2 more sources

Profiling of the metabolic transcriptome via single molecule molecular inversion probes [PDF]

Scientific Reports, 2017
AbstractCancer-specific metabolic alterations are of high interest as therapeutic targets. These alterations vary between tumor types, and to employ metabolic targeting to its fullest potential there is a need for robust methods that identify candidate targetable metabolic pathways in individual cancers.
Bitter, T.J.J. de, Water, C. van de, Heuvel, C.N.A.M. van den, Zeelen, C., Eijkelenboom, A., Tops, B.B., Oosterwijk, E., Kolev, D., Mulders, P.F.A., Laan, M. ter, Lith, S.A. van, Leenders, W.P.   +11 more
openaire   +5 more sources