Results 251 to 260 of about 5,075,651 (313)

Optimizing photoactivation of PA‐mCherry for optical pooled CRISPR screens

FEBS Open Bio, EarlyView.
Photoactivatable PA‐mCherry finds widespread use to optically tag individual cells. However, confocal 405 nm UV laser‐scanning (normal scan) is much less efficient than widefield UV illumination, limiting the use of PA‐mCherry on confocal instruments. We remedy this limitation by reporting that rapid and repeated confocal scanning with a low‐intensity,
Sravasti Mukherjee, Giulia Zanetti, Bram van den Broek, Kees Jalink   +3 more
wiley   +1 more source

RoundMi: A quantitative method to analyze mitochondrial morphology in mitotic cells

FEBS Open Bio, EarlyView.
RoundMi is a workflow for rapid analysis of mitochondrial morphology in mitotic cells. By combining adaptive preprocessing with automated segmentation and quantification, it enables accurate measurements from single focal plane images, reducing acquisition time and computational demands while remaining compatible with high‐throughput fixed and live ...
Elmira Parvindokht Bararpour, Christine Volmert, Valentina Piano   +2 more
wiley   +1 more source

Versatile vector tools for efficient protein screening across multiple expression systems

FEBS Open Bio, EarlyView.
A unified vector toolkit enables rapid protein expression screening across E. coli, insect, and mammalian cells. A single primer pair amplifies the target gene, which is inserted into any vector via a standardized interface. This streamlined workflow eliminates repeated cloning steps, accelerating the identification of optimal expression conditions for
Zhimin Zhu, Yaqing Liu, Lei Qin, Feng Yu, Qisheng Wang, Yahui Liu   +5 more
wiley   +1 more source

UiO‐66 metal–organic frameworks in biomedicine: From structural tunability to bioimaging, photodiagnostics, and photodynamic cancer therapy

FEBS Open Bio, EarlyView.
UiO‐66(Zr) metal–organic frameworks are chemically stable, biocompatible, and highly tunable nanomaterials. Their modular structure enables controlled drug delivery, multimodal bioimaging, and light‐activated photodynamic therapy, supporting integrated diagnostic and therapeutic (theranostic) applications in cancer and biomedical research.
Veronika Huntošová, Grigorii Rakhalskii, Miroslav Almáši   +2 more
wiley   +1 more source

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Molecular Mapping in the CNS

Current Pharmaceutical Design, 2002
Since ancient times the operation of the brain has elicited more than usual interest. Data mining of the human genome is revealing that many CNS abnormalities have a genetic component. As yet this information can not be used directly to cure or ameliorate specific CNS disorders although this is regarded as having great potential for future therapies ...
Lloyd, Edward J., Tehan, Benjamin G., Wong, Margaret Gail   +2 more
openaire   +3 more sources

Molecular Surface Maps

IEEE Transactions on Visualization and Computer Graphics, 2017
We present Molecular Surface Maps, a novel, view-independent, and concise representation for molecular surfaces. It transfers the well-known world map metaphor to molecular visualization. Our application maps the complex molecular surface to a simple 2D representation through a spherical intermediate, the Molecular Surface Globe.
Michael Krone, Florian Friess, Katrin Scharnowski, Guido Reina, Silvia Fademrecht, Tobias Kulschewski, Jürgen Pleiss, Thomas Ertl   +7 more
openaire   +2 more sources

Molecular Mapping of Lipoarabinomannans on Mycobacteria

Langmuir, 2009
Although the chemical composition of mycobacterial cell walls is well known, the 3D organization of the various constituents is not fully understood. In particular, it is unclear whether the major wall component lipoarabinomannan (LAM) is exposed on the outermost surface or hindered by other constituents such as mycolic acids. To address this pertinent
Verbelen, Claire, Christiaens, Nicolas, Alsteens, David, Dupres, Vincent, Baulard, Alain R, Dufrêne, Yves   +5 more
openaire   +3 more sources

A MOLECULAR MARKER MAP FOR ROSES

Acta Horticulturae, 2001
n addition to an existing core map for diploid roses which comprised 305 molecular markers 60 additional markers were mapped to extend the map. As a first application of the information contained in the map, the map position of a resistance gene from roses, Rdr1, was determined by identifying closely linked markers on linkage group 1.
Debener, T., Mattiesch, L., Vosman, B.
openaire   +1 more source

Molecular map of the desmosomal plaque

Journal of Cell Science, 1999
ABSTRACT Recent biochemical and molecular approaches have begun to establish the protein interactions that lead to desmosome assembly. To determine whether these associations occur in native desmosomes we have performed ultrastructural localisation of specific domains of the major desmosomal components and have used the results to ...
A J, North, W G, Bardsley, J, Hyam, E A, Bornslaeger, H C, Cordingley, B, Trinnaman, M, Hatzfeld, K J, Green, A I, Magee, D R, Garrod   +9 more
openaire   +2 more sources

MOLECULAR MAPPING AND MOLECULAR CHARACTERIZATION OF CROPS

2023
Agriculture is the core of the functioning of the world. From ancient times many new techniques have been introduced to produce good quality crops. From the 18th century the improvement in biotechnology and the study of genes have paved the new paths for the development in agriculture.
Sristhi Yadav, Deep Nithun Senathipathi, Jyoti Barthawal, Vasudha Kak, Gururaj Moorthy, Kunal Kishore   +5 more
openaire   +1 more source