Results 61 to 70 of about 1,088,260 (325)

Applying the auxin-based degron system for the inducible, reversible and complete protein degradation in Komagataella phaffii

iScience, 2022
Summary: The auxin-inducible degron (AID) system is a useful technique to rapidly deplete any protein of interest “on-demand.” In this study, we successfully established the AID system for the “biotech” yeast Komagataella phaffii.
Leonie Lehmayer, Lukas Bernauer, Anita Emmerstorfer-Augustin   +2 more
doaj   +1 more source

Synthetic microbial ecosystems : an exciting tool to understand and apply microbial communities [PDF]

, 2013
Many microbial ecologists have described the composition of microbial communities in a plenitude of environments, which has greatly improved our basic understanding of microorganisms and ecosystems.
Boon, Nico, De Roy, Karen, Marzorati, Massimo, Van de Wiele, Tom, Van den Abbeele, Pieter   +4 more
core   +2 more sources

CDK11 inhibition induces cytoplasmic p21WAF1 splice variant by p53 stabilisation and SF3B1 inactivation

Molecular Oncology, EarlyView.
CDK11 inhibition stabilises the tumour suppressor p53 and triggers the production of an alternative p21WAF1 splice variant p21L, through the inactivation of the spliceosomal protein SF3B1. Unlike the canonical p21WAF1 protein, p21L is localised in the cytoplasm and has reduced cell cycle‐blocking activity.
Radovan Krejcir, Lukasz Arcimowicz, Lucia Martinkova, Vaclav Hrabal, Filip Zavadil Kokas, Tomas Henek, Martina Kucerikova, Ondrej Bonczek, Pavlina Zatloukalova, Lenka Hernychova, Philip J. Coates, Borivoj Vojtesek, David P. Lane   +12 more
wiley   +1 more source

Proteolysis of Rab32 by Salmonella GtgE induces an inactive GTPase conformation

iScience, 2021
Summary: Rab GTPases are central regulators of intracellular vesicular trafficking. They are frequently targeted by bacterial pathogens through post-translational modifications. Salmonella typhimurium secretes the cysteine protease GtgE during infection,
Sergey Savitskiy, Rudolf Wachtel, Danial Pourjafar-Dehkordi, Hyun-Seo Kang, Vanessa Trauschke, Don C. Lamb, Michael Sattler, Martin Zacharias, Aymelt Itzen   +8 more
doaj   +1 more source

Nitrification-denitrification in WSP: a mechanism for permanent nitrogen removal in maturation ponds [PDF]

, 2009
A pilot-scale primary maturation pond was spiked with 15N-labelled ammonia (15NH4Cl) and 15N labelled nitrite (Na15NO2), in order to improve current understanding of the dynamics of inorganic nitrogen transformations and removal in WSP systems.
Camargo Valero, M, Curtis, T, Davenport, R, Mara, D, Newton, R, Read L F   +5 more
core  

Diverse reductive dehalogenases are associated with Clostridiales-enriched microcosms dechlorinating 1,2-dichloroethane [PDF]

, 2015
The achievement of successful biostimulation of active microbiomes for the cleanup of a polluted site is strictly dependent on the knowledge of the key microorganisms equipped with the relevant catabolic genes responsible for the degradation process.
Balloi, Annalisa, Carpani, Giovanna, Daffonchio, Daniele, de Ferra, Francesca, Lavazza, Davide, Mapelli, Francesca, Marzorati, Massimo, Merlino, Giuseppe, Rizzi, Aurora   +8 more
core   +5 more sources

Effect of chemotherapy on passenger mutations in metastatic colorectal cancer

Molecular Oncology, EarlyView.
Changes in passenger mutation load and predicted immunotherapy response after chemotherapy treatment. Tumor cells rich with passenger mutations have increased sensitivity to chemotherapy. Correlation of passenger mutations with neoantigen load suggests highly mutated clones promote a more effective response to immunotherapy, and therefore, first‐line ...
Marium T. Siddiqui, Matthew A. Cottam, Muhammad Bilal Mirza, Keeli B. Lewis, Kristen K. Ciombor, Mary Kay Washington, Kamran Idrees   +6 more
wiley   +1 more source

On the kinetic and allosteric regulatory properties of the ADP-glucose pyrophosphorylase from Rhodococcus jostii: An approach to evaluate glycogen metabolism in oleaginous bacteria [PDF]

, 2016
Rhodococcus spp. are oleaginous bacteria that accumulate glycogen during exponential growth. Despite the importance of these microorganisms in biotechnology, little is known about the regulation of carbon and energy storage, mainly the relationship ...
Alvarez, Hector Manuel, Asención Diez, Matías Damián, Cereijo, Antonela Estefanía, Dávila Costa, José Sebastián, Iglesias, Alberto Alvaro   +4 more
core   +1 more source

Molecular characterisation of human penile carcinoma and generation of paired epithelial primary cell lines

Molecular Oncology, EarlyView.
Generation of two normal and tumour (cancerous) paired human cell lines using an established tissue culture technique and their characterisation is described. Cell lines were characterised at cellular, protein, chromosome and gene expression levels and for HPV status.
Simon Broad, Mahmood Hachim, Tom Bertin, Karolina Penderecka, Rifat Hamoudi, Saif Khan, Rui Henrique, Natalie Bergmoser, Manit Arya, Kalle Sipila, Matteo Vietri Rudan, Asif Muneer, Aamir Ahmed   +12 more
wiley   +1 more source

An outer membrane determinant for RNA phage genome entry in Pseudomonas aeruginosa

iScience
Summary: Host range of a phage is determined at the various life cycle stages during phage infection. We reported the limited phage-receptor interaction between the RNA phage, PP7 and its host Pseudomonas aeruginosa strains: PAO1 has susceptible type IV ...
Hee-Won Bae, Shin-Yae Choi, You-Hee Cho
doaj   +1 more source