Results 61 to 70 of about 1,092,921 (340)

Protocol for the generation of HIV-1 genomic RNA with altered levels of N6-methyladenosine

STAR Protocols, 2022
Summary: N6-methyladenosine (m6A) modification of human immunodeficiency virus type 1 (HIV-1) RNA plays a critical role in regulating viral replication and evasion of innate immunity.
Stacia Phillips, Alice Baek, Sanggu Kim, Shuliang Chen, Li Wu   +4 more
doaj   +1 more source

Combined 5‐aminolevulinic acid and ferric ammonium citrate treatment promotes hair follicle growth by activating dermal papilla cells

FEBS Open Bio, EarlyView.
5‐Aminolevulinic acid combined with ferric ammonium citrate (5‐ALA/FAC) stimulates dermal papilla cell activity and promotes hair follicle growth. The treatment enhances ERK and AKT signaling, increases hair‐inductive gene expression, and restores dermal papilla function suppressed by dihydrotestosterone and oxidative stress, resulting in enhanced hair
Han‐Wook Ryu, Eok‐Soo Oh, Sewoon Kim
wiley   +1 more source

Applying the auxin-based degron system for the inducible, reversible and complete protein degradation in Komagataella phaffii

iScience, 2022
Summary: The auxin-inducible degron (AID) system is a useful technique to rapidly deplete any protein of interest “on-demand.” In this study, we successfully established the AID system for the “biotech” yeast Komagataella phaffii.
Leonie Lehmayer, Lukas Bernauer, Anita Emmerstorfer-Augustin   +2 more
doaj   +1 more source

Molecular biology techniques as a tool for detection and characterisation of Mycobacterium avium subsp. paratuberculosis [PDF]

, 2002
Mycobacterium avium subsp. paratuberculosis (M. paratuberculosis) is the causative agent of paratuberculosis, also known as Johne’s disease, a chronic intestinal infection in cattle and other ruminants.
Englund, Stina
core  

Sudden anaerobization in Amphibacillus xylanus increases intracellular labile ferrous iron and inhibits cell growth

FEBS Open Bio, EarlyView.
Abruptly changing from aerobic to anaerobic conditions (sudden anaerobization) induced growth inhibition and a significant increase in intracellular labile ferrous iron in the aerotolerant anaerobe Amphibacillus xylanus. We found that free flavins mediate efficient electron transfer from NADH to ferric iron under anaerobic conditions, suggesting that ...
Shinya Kimata, Yoichi Sakai, Keisuke Tanaka, Etsuro Yoshimura, Ken Kitano, Morio Ishikawa, Tadahiro Nakamoto, Tsutomu Takayama, Hirofumi Inoue, Kaho Nomura, Tomonori Suzuki, Masataka Uchino, Akira Abe, Youichi Niimura   +13 more
wiley   +1 more source

Virulence Regulation in Borrelia burgdorferi

Microorganisms
Borrelia burgdorferi, the causative agent of Lyme disease, is the most common vector-borne disease in the United States. Compared with other bacterial pathogens, B. burgdorferi has many unique features.
Sierra George, Zhiming Ouyang
doaj   +1 more source

Proteolysis of Rab32 by Salmonella GtgE induces an inactive GTPase conformation

iScience, 2021
Summary: Rab GTPases are central regulators of intracellular vesicular trafficking. They are frequently targeted by bacterial pathogens through post-translational modifications. Salmonella typhimurium secretes the cysteine protease GtgE during infection,
Sergey Savitskiy, Rudolf Wachtel, Danial Pourjafar-Dehkordi, Hyun-Seo Kang, Vanessa Trauschke, Don C. Lamb, Michael Sattler, Martin Zacharias, Aymelt Itzen   +8 more
doaj   +1 more source

Natural Products as Geroprotective Modulators in Diabetic Nephropathy: A Mechanistic Framework Integrating Aging Hallmarks and the AMPK–SIRT1–Nrf2 Axis

Aging and Cancer, EarlyView.
Natural products target the aging kidney in diabetic nephropathy by restoring the AMPK–SIRT1–Nrf2 axis, reducing oxidative stress, inflammation, fibrosis, and cellular senescence while enhancing mitochondrial biogenesis and antioxidant defenses.
Sherif Hamidu, Latif Adams, Eunice Ampem‐Danso, Justice Kumi, Abigail Aning, Fatima Hamidu, Mustapha Abdul Mumin Mohammed, Ayuba Nii Darku Ankrah, Seth Kwabena Amponsah   +8 more
wiley   +1 more source

Microbiome and Dental Changes in Horses Fed a High Soluble Carbohydrate Diet

Animals
This study investigated the oral microbiome of horses maintained on a high soluble carbohydrate diet based on sugarcane, in comparison to those fed a pasture-based diet composed of Cynodon spp., aiming to identify associations between dietary intake, the
Milena Domingues Lacerenza, Júlia de Assis Arantes, Gustavo Morandini Reginato, Gabriela Luiza Fagundes Finardi, Pedro Henrique Marchi, Thiago Henrique Annibale Vendramini, Rodrigo Romero Corrêa, Pamela Aparecida Maldaner Pereira, Carlos Augusto Araújo Valadão, Renata Gebara Sampaio Dória   +9 more
doaj   +1 more source

Crosstalk between the ancestral type VII secretion system ESX-4 and other T7SS in Mycobacterium marinum

iScience, 2022
Summary: The type VII secretion system (T7SS) of Mycobacterium tuberculosis secretes three substrate classes: Esx, Esp, and PE/PPE proteins, that play important roles in bacterial physiology and host interaction.
Yuchen Wang, Yuting Tang, Chen Lin, Junli Zhang, Juntao Mai, Jun Jiang, Xiaoxiao Gao, Yao Li, Guoping Zhao, Lu Zhang, Jun Liu   +10 more
doaj   +1 more source