High molecular weight insoluble parkin in the substantia nigra of patients with idiopathic Parkinson's disease. [PDF]
Tremblay C +9 more
europepmc +1 more source
We describe detailed protocols for the purification and preparation of Marchantia polymorpha Auxin Response Factor 2 (MpARF2). This protein is fused to an MBP solubility tag and an mNG fluorescent tag and is purified from Escherichia coli. The presented procedures make it possible to study MpARF2 assemblies, which could arise from phase separation ...
Bas Janssen +5 more
wiley +1 more source
Electrospun Polyvinylpyrrolidone Fibers for Fast-Dissolving Drug Delivery: Defining the Viscosity Window and Evaluating the Role of Molecular Weight. [PDF]
Uhljar LÉ, Tagscherer ZV, Ambrus R.
europepmc +1 more source
Protocol for quantifying miRNA trafficking across the endosomal membrane
An in vitro protocol measures miRNA uptake into endosomes isolated from mammalian cell extracts, which are free of subcellular contaminants. Performed at 37 °C in the presence of ATP, it ensures the import of single‐stranded miRNA into the endosomal lumen.
Syamantak Ghosh +2 more
wiley +1 more source
Therapeutic role of low molecular weight heparin and fat-soluble vitamins in pregnancy-associated prethrombotic conditions. [PDF]
Yanhong H, Cuiyi H, Ruiling Y, Guowei H.
europepmc +1 more source
Research is strongest when conducted alongside patients, not just about them. Patient research organizations help integrate patient perspectives into research priorities, study design, and scientific meetings, leading to meaningful patient outcomes and development of relevant therapies.
Jenica H. Kakadia +9 more
wiley +1 more source
Hyaluronic Acid Molecular Weight Modulates Chitosan-Gelatin Scaffold Properties and Cancer Cell Organization in 3D Culture. [PDF]
Chanu LR, Dong GC, Lai PS.
europepmc +1 more source
Using peripheral blood for determining B‐cell or T‐cell clonality is more reliable when we use cell‐free RNA (cfRNA) because cells release blood significantly more RNA than DNA. Next‐generation sequencing (NGS) of cfRNA allows us to evaluate fragment cfRNA and evaluate clonality reliably without the need for prior determination of the specific dominant
Adam Albitar +11 more
wiley +1 more source
Factors Determining the Antimicrobial Effectiveness of Chitosan: A Critical Analysis of the Impact of Molecular Weight and Degree of Deacetylation. [PDF]
Czajkowska K +3 more
europepmc +1 more source

