Results 21 to 30 of about 353 (119)

Integrated RPA–CRISPR/Cas12a Technology for Rapid Detection of Salmonella enterica [PDF]

Diagnostics
Background/Objectives: Rapid identification of foodborne pathogens is of high practical significance because it enables prompt epidemiological response, timely patient management, and effective sanitary control of food products.
Ainur Akimbekova, Aisha Shaizadinova, Meruyert Amanzholova, Aitbay Bulashev, Sailau Abeldenov   +4 more
doaj   +2 more sources

Moraxella bovoculi em casos de ceratoconjuntivite infecciosa bovina no Rio Grande do Sul [PDF]

Pesquisa Veterinária Brasileira, 2012
A ceratoconjuntivite infecciosa (CI), embora raramente fatal, resulta em perdas econômicas significativas para os rebanhos bovinos e ovinos. Os principais agentes causadores dessa enfermidade são Moraxella bovis e Moraxella ovis. Em 2007 foi descrita uma
Felipe Libardoni, Charles F.C. Scherer, Luana Farias, Andréia Vielmo, Claudia Balzan, Agueda C. de Vargas   +5 more
doaj   +3 more sources

Antimicrobial Activity of Ultraviolet C Light as a Potential Novel Treatment for Moraxella bovis Infection-An In Vitro Study. [PDF]

Vet Ophthalmol
ABSTRACT Objective To investigate the antimicrobial effect of 275 nm wavelength ultraviolet C (UV‐C) light on M. bovis using an affordable, broadly available modified handheld device and to determine if photoreactivation plays a role in bacterial resistance to UV‐C. Methods UV‐C therapy (UVCT) was evaluated in vitro using triplicates of 3 M.
Turicea B, Sahoo DK, Allbaugh RA, Stinman CC, Kubai MA.   +4 more
europepmc   +2 more sources

Relatedness of type IV pilin PilA amongst geographically diverse Moraxella bovoculi isolated from cattle with infectious bovine keratoconjunctivitis. [PDF]

J Med Microbiol, 2021
Introduction.Moraxella bovoculiis frequently isolated from the eyes of cattle with infectious bovine keratoconjunctivitis (IBK; pinkeye). As withM. bovis,which has been causally linked to IBK,M. bovoculiexpresses an RTX (repeats in the structural toxin) cytotoxin that is related toM. boviscytotoxin. Pilin, another pathogenic factor inM.
Angelos JA, Clothier KA, Agulto RL, Mandzyuk B, Tryland M.   +4 more
europepmc   +6 more sources

Isolation of Moraxella bovoculi from racehorses with keratoconjunctivitis [PDF]

Journal of Veterinary Diagnostic Investigation, 2014
Moraxella bovoculi was isolated and identified in ocular fluid samples collected from 9 racehorses with infectious keratoconjunctivitis in China in 2013. All 9 M. bovoculi isolates were hemolytic, Gram-negative diplococci that were phenylalanine deaminase positive.
Huimin, Liu, Jing, Yan, Yutian, Wang, Qi, Yan, Linping, Zhao, Ruoqian, Yan, Hongxuan, He   +6 more
openaire   +2 more sources

The engineered CRISPR‐Mb2Cas12a variant enables sensitive and fast nucleic acid‐based pathogens diagnostics in the field

Plant Biotechnology Journal, Volume 21, Issue 7, Page 1465-1478, July 2023., 2023
Summary Existing CRISPR/Cas12a‐based diagnostic platforms offer accurate and vigorous monitoring of nucleic acid targets, but have the potential to be further optimized for more efficient detection. Here, we profiled 16 Cas12a orthologs, focusing on their trans‐cleavage activity and their potential as diagnostic enzymes.
Jian Jiao, Yiqi Liu, Mengli Yang, Jingcheng Zheng, Chonghuai Liu, Wenxiu Ye, Shangwei Song, Tuanhui Bai, Chunhui Song, Miaomiao Wang, Jiangli Shi, Ran Wan, Kunxi Zhang, Pengbo Hao, Jiancan Feng, Xianbo Zheng   +15 more
wiley   +1 more source

A Five Year Randomized Controlled Trial to Assess the Efficacy and Antibody Responses to a Commercial and Autogenous Vaccine for the Prevention of Infectious Bovine Keratoconjunctivitis

Vaccines, 2022
A randomized control trial was performed over a five-year period to assess the efficacy and antibody response induced by autogenous and commercial vaccine formulations against infectious bovine keratoconjunctivitis (IBK).
Matthew M. Hille, Matthew L. Spangler, Michael L. Clawson, Kelly D. Heath, Hiep L. X. Vu, Rachel E. S. Rogers, John Dustin Loy   +6 more
doaj   +1 more source

Application of shotgun metagenomics sequencing and targeted sequence capture to detect circulating porcine viruses in the Dutch–German border region

Transboundary and Emerging Diseases, Volume 69, Issue 4, Page 2306-2319, July 2022., 2022
Abstract Porcine viruses have been emerging in recent decades, threatening animal and human health, as well as economic stability for pig farmers worldwide. Next‐generation sequencing (NGS) can detect and characterize known and unknown viruses but has limited sensitivity when an unbiased approach, such as shotgun metagenomics sequencing, is used.
Leonard Schuele, Erley Lizarazo‐Forero, Katrin Strutzberg‐Minder, Sabine Schütze, Sandra Löbert, Claudia Lambrecht, Jürgen Harlizius, Alex W. Friedrich, Silke Peter, John W. A. Rossen, Natacha Couto   +10 more
wiley   +1 more source

Strong and tunable anti‐CRISPR/Cas activities in plants

Plant Biotechnology Journal, Volume 20, Issue 2, Page 399-408, February 2022., 2022
Summary CRISPR/Cas has revolutionized genome engineering in plants. However, the use of anti‐CRISPR proteins as tools to prevent CRISPR/Cas‐mediated gene editing and gene activation in plants has not been explored yet. This study describes the characterization of two anti‐CRISPR proteins, AcrIIA4 and AcrVA1, in Nicotiana benthamiana.
Camilo Calvache, Marta Vazquez‐Vilar, Sara Selma, Mireia Uranga, Asun Fernández‐del‐Carmen, José‐Antonio Daròs, Diego Orzáez   +6 more
wiley   +1 more source

Expanding the range of editable targets in the wheat genome using the variants of the Cas12a and Cas9 nucleases

Plant Biotechnology Journal, Volume 19, Issue 12, Page 2428-2441, December 2021., 2021
Summary The development of CRISPR‐based editors recognizing distinct protospacer‐adjacent motifs (PAMs), or having different spacer length/structure requirements broadens the range of possible genomic applications. We evaluated the natural and engineered variants of Cas12a (FnCas12a and LbCas12a) and Cas9 for their ability to induce mutations in ...
Wei Wang, Bin Tian, Qianli Pan, Yueying Chen, Fei He, Guihua Bai, Alina Akhunova, Harold N. Trick, Eduard Akhunov   +8 more
wiley   +1 more source