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A series of experiments was carried out to determine the accuracy and reproducibility of the most probable number (MPN) technique for estimating concentrations of viable planktonic diatoms from sediment samples. Two contrasting species were used to inoculate sterilised sediments: Ditylum brightwellii (large single-celled species) and Skeletonema ...
A.S.D. Harris, K.J. Jones, J. Lewis
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Enumeration of Specific Populations by the Most-Probable-Number (MPN) Method
1979The most-probable-number (MPN) method of bacteria enumeration dates back to the earliest days of bacteriology. Despite drawbacks of the method, it offers a relatively simple and useful means of estimating microbial populations, especially for determining the physiological potential of microbial biomass.
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A Most Probable Number (MPN) method was developed allowing for the first time estimation of populations of bacteria capable of heterotrophic nitrification. The method was applied to an acidic soil of a coniferous forest exhibiting nitrate production.
H. Papen, R. von Berg
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Water Research, 2008
Most probable number (MPN) and colony-forming-unit (CFU) estimates of fecal coliform bacteria concentration are common measures of water quality in coastal shellfish harvesting and recreational waters. Estimating procedures for MPN and CFU have intrinsic variability and are subject to additional uncertainty arising from minor variations in experimental
Andrew Gronewold, Robert Wolpert
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Most probable number (MPN) and colony-forming-unit (CFU) estimates of fecal coliform bacteria concentration are common measures of water quality in coastal shellfish harvesting and recreational waters. Estimating procedures for MPN and CFU have intrinsic variability and are subject to additional uncertainty arising from minor variations in experimental
Andrew Gronewold, Robert Wolpert
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Applied Microbiology and Biotechnology, 2014
We developed a sensitive quantitative assay for detecting Ralstonia solanacearum in soil by most probable number (MPN) analysis based on bio-PCR results. For development of the detection method, we optimized an elution buffer containing 5 g/L skim milk for extracting bacteria from soil and reducing contamination of polymerase inhibitors in soil ...
Yasuhiro Inoue
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We developed a sensitive quantitative assay for detecting Ralstonia solanacearum in soil by most probable number (MPN) analysis based on bio-PCR results. For development of the detection method, we optimized an elution buffer containing 5 g/L skim milk for extracting bacteria from soil and reducing contamination of polymerase inhibitors in soil ...
Yasuhiro Inoue
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Soil Biology and Biochemistry, 1981
Abstract A rapid, precise and accurate microtiter most-probable-number (MPN) procedure has been developed and tested for simultaneous (two reagent addition) enumeration of denitrifier and nitrate reducer populations in an agricultural soil. Values from 0.12 × 10 6 to 0.22 × 10 6 denitrifiers g −1 soil were determined using two concentrations of ...
T.E. Staley, J.B. Griffin
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Abstract A rapid, precise and accurate microtiter most-probable-number (MPN) procedure has been developed and tested for simultaneous (two reagent addition) enumeration of denitrifier and nitrate reducer populations in an agricultural soil. Values from 0.12 × 10 6 to 0.22 × 10 6 denitrifiers g −1 soil were determined using two concentrations of ...
T.E. Staley, J.B. Griffin
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Journal of Microbiological Methods, 1998
Abstract An adjustment of DAPI direct count (DDC) technique for bacteria was developed for enumeration of protozoa. A comparison between the DDC technique and the MPN method was then conducted in a determination of the number of protozoa in porous media.
Tor Kristian Stevik +2 more
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Abstract An adjustment of DAPI direct count (DDC) technique for bacteria was developed for enumeration of protozoa. A comparison between the DDC technique and the MPN method was then conducted in a determination of the number of protozoa in porous media.
Tor Kristian Stevik +2 more
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A general purpose program for obtaining most probable number tables
4 páginas.-- 2 figuras.-- 6 referencias.-- El autor González Grau, Juan Miguel pertenece actualmente al Instituto de Recursos Naturales y Agrobiología de SevillaThe most-probable number (MPN) method is frequently used for counting microorganisms.
Juan Miguel Gonzalez
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Estimation of methane-producing bacterial numbers by the most probable number (MPN) technique
Water Research, 1967Abstract Heukelekian and Heinemann proposed a method in 1939 of enumerating the methane-producing bacteria that occur in anaerobic digesters. A modification of their method is presented here which is simpler to use and yields more consistent results.
M SIEBERT, W HATTINGH
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