Results 51 to 60 of about 140,266 (262)

Patient therapy outcome modeling in cancer organoids is improved by cancer‐associated fibroblasts and organoid assembly convolution

open access: yesMolecular Oncology, EarlyView.
Patient‐derived organoids (PDOs) from pancreatic, colorectal, and gastric cancers were used to evaluate standard and experimental therapies. Incorporating cancer‐associated fibroblasts (CAFs) into organoid cultures improved patient therapy outcome prediction.
Marcin Grochowski   +12 more
wiley   +1 more source

Automated FRAP microscopy for high‐throughput analysis of protein dynamics in chromatin organization and transcription

open access: yesFEBS Open Bio, EarlyView.
RoboMic is an automated confocal microscopy pipeline for high‐throughput functional imaging in living cells. Demonstrated with fluorescence recovery after photobleaching (FRAP), it integrates AI‐driven nuclear segmentation, ROI selection, bleaching, and analysis.
Selçuk Yavuz   +6 more
wiley   +1 more source

Polarization‐resolved femtosecond Vis/IR spectroscopy tailored for resolving weak signals in biological samples using minimal sample volume

open access: yesFEBS Open Bio, EarlyView.
Unique biological samples, such as site‐specific mutant proteins, are available only in limited quantities. Here, we present a polarization‐resolved transient infrared spectroscopy setup with referencing to improve signal‐to‐noise tailored towards tracing small signals. We provide an overview of characterizing the excitation conditions for polarization‐
Clark Zahn, Karsten Heyne
wiley   +1 more source

A new efficient adaptive rood pattern search motion estimation algorithm

open access: yesНаучно-технический вестник информационных технологий, механики и оптики
Motion estimation plays a crucial role in video coding; the Adaptive Rood Pattern Search (ARPS) algorithm is a well known fast motion estimation algorithm.
Sh. A. Shaker   +2 more
doaj   +1 more source

EFFICIENT BLOCK MATCHING ALGORITHMS FOR MOTION ESTIMATION IN H.264/AVC [PDF]

open access: yesICTACT Journal on Image and Video Processing, 2015
In Scalable Video Coding (SVC), motion estimation and inter-layer prediction play an important role in elimination of temporal and spatial redundancies between consecutive layers.
P. Muralidhar, C.B. Rama Rao
doaj  

Relative Importance of Binocular Disparity and Motion Parallax for Depth Estimation: A Computer Vision Approach

open access: yesRemote Sensing, 2019
Binocular disparity and motion parallax are the most important cues for depth estimation in human and computer vision. Here, we present an experimental study to evaluate the accuracy of these two cues in depth estimation to stationary objects in a static
Mostafa Mansour   +3 more
doaj   +1 more source

Divergence-Free Motion Estimation [PDF]

open access: yes, 2012
This paper describes an innovative approach to estimate motion from image observations of divergence-free flows. Unlike most state-of-the-art methods, which only minimize the divergence of the motion field, our approach utilizes the vorticity-velocity formalism in order to construct a motion field in the subspace of divergence free functions.
Herlin, Isabelle   +3 more
openaire   +1 more source

A light‐triggered Time‐Resolved X‐ray Solution Scattering (TR‐XSS) workflow with application to protein conformational dynamics

open access: yesFEBS Open Bio, EarlyView.
Time‐resolved X‐ray solution scattering captures how proteins change shape in real time under near‐native conditions. This article presents a practical workflow for light‐triggered TR‐XSS experiments, from data collection to structural refinement. Using a calcium‐transporting membrane protein as an example, the approach can be broadly applied to study ...
Fatemeh Sabzian‐Molaei   +3 more
wiley   +1 more source

Analysing the significance of small conformational changes and low occupancy states in serial crystallographic data

open access: yesFEBS Open Bio, EarlyView.
This protocol paper outlines methods to establish the success of a time‐resolved serial crystallographic experiment, by means of statistical analysis of timepoint data in reciprocal space and models in real space. We show how to amplify the signal from excited states to visualise structural changes in successful experiments.
Jake Hill   +4 more
wiley   +1 more source

Decreased cold‐sensing function of the transient receptor potential channel TRPM8 from tailed amphibians

open access: yesFEBS Open Bio, EarlyView.
Despite frogs avoiding low temperatures, examination of four salamander species revealed that none avoided cold and all possessed cold tolerance. Functional analysis of TRPM8, a cold sensor, showed that all salamander TRPM8s had lost their cold sensitivity.
Tadahiro Sawao   +3 more
wiley   +1 more source

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