Evolution‐guided yeast complementation reveals functional differences in human PSPH variants
Ancient genomes can help guide which human genetic variants are tested experimentally. This study applies that idea to PSPH, a gene involved in serine biosynthesis, and uses high‐throughput yeast complementation to compare variant function. The findings reveal measurable differences among selected alleles and illustrate the value of evolution‐guided ...
Mauricio Campa‐Álvarez +6 more
wiley +1 more source
Hybrid LTCC-Polyimide Approach for High-Sensitivity Mechanical Sensing Applications. [PDF]
Tounsi F +5 more
europepmc +1 more source
We report the first β‐glucosidase with demonstrated hydrolytic activity on an N‐linked glycopyranoside. The enzyme, native to maize, was biochemically characterised for this novel reaction, and structural modelling of the enzyme–substrate complex revealed several clues to the underlying reduced catalytic rate relative to its native O‐glycopyranoside ...
Hani Gharabli +3 more
wiley +1 more source
Molecular Investigation of <i>Eimeria</i> spp. Infection in Weaned Dairy Calves in Thessaly, Greece, and Associated Risk Factors. [PDF]
Arsenopoulos KV +2 more
europepmc +1 more source
In silico and in vitro exploration of a tyrosinase for biocatalytic production of catechols
Tyrosinase from Ralstonia pseudosolanacearum is a promising biocatalyst for producing valuable catechols from monophenol substrates. This tyrosinase is uniquely suited to this due to its high monophenolase : diphenolase ratio. We combined in silico docking and in vivo kinetic characterisation of this tyrosinase with 11 industrially relevant monophenols,
James Britton +6 more
wiley +1 more source
Status of Beekeeping Industry in Tanzania: Resources, Practices, and Conservation. [PDF]
Mussa IS +3 more
europepmc +1 more source
Adenosine triphosphate as a modulator of protein interactions and stability
ATP is best known as the cell's energy currency, but it also shapes how proteins fold, interact, aggregate and form biomolecular condensates. This review explains the emerging physical principles behind these effects, including weak binding to charged protein regions, magnesium‐dependent behaviour and concentration‐dependent control of protein ...
Shuyuan Tan, Robin Curtis
wiley +1 more source
Development of a Low-Cost Portable System for Text-to-Speech Conversion: A Mechanical-Aided Optical Solution for Educational Inclusion. [PDF]
Rentería L +4 more
europepmc +1 more source
Nonvolatile silicon photonic MEMS switches enabled by van der Waals force. [PDF]
Hu Y +9 more
europepmc +1 more source
Laser-Assisted Diamond Turning for Anisotropy Suppression in Calcium Fluoride. [PDF]
Xing E +10 more
europepmc +1 more source

