Results 71 to 80 of about 105,984 (266)

Dynamic Contact Analysis of Flexible Telescopic Boom Systems with Moving Boundary

open access: yesMathematics
A flexible telescopic boom is a multi-body system composed of several hollow booms nestled into each other. For this kind of system, due to the limitation of the elemental size being fixed, it is necessary to divide it into many small-sized elements and ...
Tianjiao Zhao, Zhaohui Qi, Tianyu Wang
doaj   +1 more source

Automated FRAP microscopy for high‐throughput analysis of protein dynamics in chromatin organization and transcription

open access: yesFEBS Open Bio, EarlyView.
RoboMic is an automated confocal microscopy pipeline for high‐throughput functional imaging in living cells. Demonstrated with fluorescence recovery after photobleaching (FRAP), it integrates AI‐driven nuclear segmentation, ROI selection, bleaching, and analysis.
Selçuk Yavuz   +6 more
wiley   +1 more source

Analytical Solution for Rheological Consolidation of Soft Clay with Threshold Hydraulic Gradient

open access: yes工程科学与技术, 2019
The influence of a threshold gradient of water flow in soft clays on consolidation behavior has been gradually recognized. However, the consolidation theory with consideration of a threshold hydraulic gradient and rheological characteristics has rarely ...
Chuanxun LI, Haotian MA, Dandan JIN
doaj  

Polarization‐resolved femtosecond Vis/IR spectroscopy tailored for resolving weak signals in biological samples using minimal sample volume

open access: yesFEBS Open Bio, EarlyView.
Unique biological samples, such as site‐specific mutant proteins, are available only in limited quantities. Here, we present a polarization‐resolved transient infrared spectroscopy setup with referencing to improve signal‐to‐noise tailored towards tracing small signals. We provide an overview of characterizing the excitation conditions for polarization‐
Clark Zahn, Karsten Heyne
wiley   +1 more source

Implementation and Validation of Explicit Immersed Boundary Method and Lattice Boltzmann Flux Solver in OpenFOAM

open access: yesDynamics
In this work, the explicit boundary-condition-enforced immersed boundary method (EIBM) and the lattice Boltzmann flux solver (LBFS) are integrated into OpenFOAM to efficiently solve incompressible flows with complex geometries and moving boundaries.
Yangyang Liu   +3 more
doaj   +1 more source

A light‐triggered Time‐Resolved X‐ray Solution Scattering (TR‐XSS) workflow with application to protein conformational dynamics

open access: yesFEBS Open Bio, EarlyView.
Time‐resolved X‐ray solution scattering captures how proteins change shape in real time under near‐native conditions. This article presents a practical workflow for light‐triggered TR‐XSS experiments, from data collection to structural refinement. Using a calcium‐transporting membrane protein as an example, the approach can be broadly applied to study ...
Fatemeh Sabzian‐Molaei   +3 more
wiley   +1 more source

On Advances of Diffuse‑Interface Immersed Boundary Method and Its Applications

open access: yesTransactions of Nanjing University of Aeronautics and Astronautics
The diffuse-interface immersed boundary method (IBM) possesses excellent capabilities for simulating flows around complex geometries and moving boundaries.
YANG Liming   +4 more
doaj   +1 more source

Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies

open access: yesFEBS Open Bio, EarlyView.
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli   +8 more
wiley   +1 more source

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