Italian Journal of Animal Science, 2010 This study investigated the effects of a multienzyme complex containing protease and carbohydrase enzymes on the performance and nutrient digestibility of broiler chickens under different rearing conditions from 1 to 42 d of age. Two experimental starter
Fuensanta Hernández +3 more
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Automated Multienzyme Digestion Workflows for Confident Characterization of Challenging Posttranslational Modifications in Therapeutic Antibodies by LC-MS/MS. [PDF]
Rapid Commun Mass SpectromABSTRACT Rationale Non‐reduced trypsin digestion of therapeutic antibodies can generate large disulfide‐linked peptide complexes that exhibit poor chromatographic and mass spectrometric performance. This limits the confident characterization of posttranslational modifications (PTMs) in specific antibody regions, such as the heavy‐chain constant region ...
Eskesen NS +10 more
europepmc +2 more sources
Effect of Storage Duration on Amylase, Protease, and Lipase Activities in Ultrasound-Assisted Extracted Bovine Pancreatin [PDF]
MoleculesLong-term stability of multienzyme protein systems is governed by preservation of conformational integrity and resistance to thermally induced structural destabilization. This study evaluated bovine pancreatin (BP) obtained by conventional extraction (CM)
Gulmira Kenenbay +3 more
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Assembly-Line Biosynthesis in Living-Cell Emulsions via Tunable Supramolecular Surface Chemistry [PDF]
Nature CommunicationsNature organizes enzymes in micro-compartments to enable efficient biosynthesis, inspiring multienzyme cascades for producing complex molecules. However, practical implementation is often limited by enzyme incompatibility, poor substrate transfer, and ...
Xiankun Wu +4 more
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A simple strategy for enhancing NADPH-dependent biocatalysis via phosphate analogue-mediated maintenance of the NADPH pool [PDF]
RSC AdvancesNADPH-dependent biocatalysis is widely used for the synthesis of valuable chemicals, and maintaining an effective NADPH pool during catalysis is critical for its performance. Although cofactor regeneration has been extensively studied, the maintenance of
Rong Liu +8 more
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Structure of the murine lysosomal multienzyme complex core. [PDF]
Sci Adv, 2021 The 3D structure of the complex formed by the enzymes GLB1 and CTSA furthers our understanding of lysosomal storage diseases.
Gorelik A +4 more
europepmc +4 more sources
Controlled Orientation of Active Sites in a Nanostructured Multienzyme Complex. [PDF]
Sci Rep, 2016 Multistep cascade reactions in nature maximize reaction efficiency by co-assembling related enzymes. Such organization facilitates the processing of intermediates by downstream enzymes.
Lim SI +7 more
europepmc +2 more sources
Scanning electrochemical microscopy for the interrogation of biologically modified surfaces [PDF]
, 2007 This thesis describes two novel applications of scanning electrochemical microscopy (SECM) to biological systems. The first involves the characterisation of a novel, impedance based genomic DNA biosensor - previously developed within the group.
Roberts, William St John
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A light-responsive multienzyme complex combining cascade enzymes within a peptide-based matrix. [PDF]
RSC Adv, 2018 A light-responsive multienzyme complex was developed by incorporating glucose oxidase and hemin within a peptide-based matrix, in which the catalytic activity of multienzyme complex can be controlled using UV and visible light.
Wang Y +6 more
europepmc +2 more sources
Studies on the arom multienzyme complex of Neurospora crassa [PDF]
, 1977 Practical conditions were developed for growing Neurospora crassa on a scale from which substantial amounts of arom multienzyme complex could be extracted.
Jamieson, Janet M
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