A Simple, Rapid, and Highly Efficient Gene Expression System for Multiheme Cytochromes c [PDF]
The genes of tetraheme cytochrome c3 and hexadecaheme high-molecular-weight cytochrome c from Desulfovibrio vulgaris could be overexpressed as holoproteins in Shewanella oneidensis TSP-C using pUC-type vectors of E. coli. Surprisingly, S. oneidensis was transformed directly by pUC-type vectors through electroporation.
K, Ozawa +3 more
openaire +2 more sources
Photoreduction of Shewanella oneidensis Extracellular Cytochromes by Organic Chromophores and Dye-Sensitized TiO2. [PDF]
The transfer of photoenergized electrons from extracellular photosensitizers across a bacterial cell envelope to drive intracellular chemical transformations represents an attractive way to harness nature's catalytic machinery for solar-assisted chemical
Ainsworth, Emma V +10 more
core +3 more sources
Deep-branching ANME-1c archaea grow at the upper temperature limit of anaerobic oxidation of methane
In seafloor sediments, the anaerobic oxidation of methane (AOM) consumes most of the methane formed in anoxic layers, preventing this greenhouse gas from reaching the water column and finally the atmosphere.
David Benito Merino +7 more
doaj +1 more source
Extracellular electron uptake in Methanosarcinales is independent of multiheme c -type cytochromes [PDF]
Abstract The co-occurrence of Geobacter and Methanosarcinales is often used as a proxy for the manifestation of direct interspecies electron transfer (DIET) in man-made and natural aquatic environments. We previously reported that not all
Yee, Mon Oo, Rotaru, Amelia-Elena
openaire +7 more sources
Impact of conductive magnetite nanoparticles and anode polarization on glucose fermentation
Abstract BACKGROUND Electrically conductive materials and electrochemical stimulation strategies are increasingly explored to steer microbial fermentation towards desired metabolic outputs. These approaches also hold remarkable potential to couple wastewater treatment (WWT) to the production of value‐added compounds, in turn enhancing environmental ...
Clara Marandola +6 more
wiley +1 more source
A suite of de novo c-type cytochromes for functional oxidoreductase engineering [PDF]
Central to the design of an efficient de novo enzyme is a robust yet mutable protein scaffold. The maquette approach to protein design offers precisely this, employing simple four-α-helix bundle scaffolds devoid of evolutionary complexity and with proven
Anderson, J L R +7 more
core +2 more sources
Reconstruction of Extracellular Respiratory Pathways for Iron(III) Reduction in Shewanella Oneidensis Strain MR-1 [PDF]
Shewanella oneidensis strain MR-1 is a facultative anaerobic bacterium capable of respiring a multitude of electron acceptors, many of which require the Mtr respiratory pathway.
Dan Coursolle, Jeffrey A. Gralnick
core +2 more sources
Abstract The QrcABCD quinone reductase complex is an electrogenic complex present in sulfate‐reducing bacteria of the Desulfobacterota phylum. It operates as a cytochrome c3:menaquinone oxidoreductase involved in electron transfer from periplasmic hydrogen or formate oxidation to the menaquinone (MK) pool.
Gonçalo Manteigas +4 more
wiley +1 more source
AbstractBiohybrid systems can combine inorganic light‐harvesting materials and whole‐cell biocatalysts to utilize solar energy for the production of chemicals and fuels. Whole‐cell biocatalysts have an intrinsic self‐repair ability and are able to produce a wide variety of multicarbon chemicals in a sustainable way with metabolic engineering.
Huijie Zhang +6 more
openaire +3 more sources
While early genetic and low-resolution structural observations suggested that extracellular conductive filaments on metal-reducing organisms such as Geobacter were composed of type IV pili, it has now been established that bacterial c-type cytochromes ...
Fengbin Wang +8 more
doaj +1 more source

