Results 111 to 120 of about 88,611 (123)
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Photoinduced-electron transfer coupled with DNA cross-chain displacement multiple amplification for fluorescence biosensing of MicroRNA

Analytica Chimica Acta, 2021
In this work, a new fluorescence biosensor platform based on distance-dependent photoinduced-electron transfer (PET) coupled with target cross-chain displacement cyclic amplification strategy was developed to detect MicroRNA. The DNA cross structure was cleverly designed to protect restriction site, then multiple amplification reactions of target cycle
Qianqian, Cai   +3 more
openaire   +2 more sources

Simple, Rapid and Sensitive Detection of Pseudomonas aeruginesa by Colorimetric Multiple Cross Displacement Amplification

Current Medical Science, 2020
Pseudomonas aeruginosa (P. aeruginosa) is a major opportunistic pathogen in hospital-acquired infections. Thus, early diagnosis is the best strategy for fighting against these infections. In this report, we incorporated multiple cross displacement amplification (MCDA) combined with the malachite green (MG) for rapid, sensitive, specific and visual ...
Dong-Xun, Li   +4 more
exaly   +3 more sources

Biosensor‐based multiple cross displacement amplification platform for visual and rapid identification of hepatitis C virus

Journal of Medical Virology
AbstractHepatitis C remains a global health problem, especially in poverty‐stricken areas. A rapid and sensitive point‐of‐care (POC) diagnostic tool is critical for the early detection and timely treatment of hepatitis C virus (HCV) infection. Here, for the first time, we reported a novel molecular diagnostic assay, termed reverse transcription ...
Xu Chen   +9 more
openaire   +2 more sources

Ultra-efficient multiple cross displacement amplification-lateral flow biosensor (MCDA-LFB) for serogroup identification of prevalent Neisseria meningitidis

Analytical Biochemistry, 2022
Meningococcal disease caused by Neisseria meningitidis remains a major global public health concern. Serogroup A, B, C and W135 were the major disease-causing serogroups. It is vital to timely and efficiently detect and differentiate these four serogroups.
Linlin Yan   +9 more
openaire   +2 more sources

Development of a real-time multiple cross displacement amplification assay for rapid and highly sensitive detection of Streptococcus pyogenes

European Journal of Clinical Microbiology & Infectious Diseases
The aim of this study was to develop a method based on multiple cross displacement amplification (MCDA) and real-time fluorescence technique for rapid, highly sensitive and specific detection of Streptococcus pyogenes (Group A Streptococcus, GAS). A set of 10 primers targeting the speB gene of GAS was designed for the MCDA reaction.
Yiqin, Zhang   +8 more
openaire   +2 more sources

Establishment of a CRISPR/Cas12b-Based Multiple Cross Displacement Amplification Assay for the Rapid, Sensitive, and Specific Detection of Brucella ovis

ACS Infectious Diseases
Brucella ovis (B. ovis), a major pathogenic species within the Brucella genus, causes ovine epididymitis. Although the isolation and identification of B. ovis remain the gold standard for diagnosis, these methods are unsuitable for early detection.
Yue Zhang   +8 more
openaire   +2 more sources

Highly Sensitive and Specific Diagnosis of Enterovirus A71 by Reverse Transcription Multiple Cross‐Displacement Amplification‐Labeled Nanoparticles Biosensor

Journal of Medical Virology
ABSTRACT Enterovirus A71 (EVA71) is a leading causative agent of hand, foot, and mouth disease, posing a significant threat to the health of young children, particularly in the Asia‐Pacific region.
Jinzhi Cheng   +4 more
openaire   +2 more sources

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