Results 231 to 240 of about 877,269 (241)

Characterization of the serum and skin inflammatory profile in canine pemphigus foliaceus using multiplex assay and quantitative real-time polymerase chain reaction (qRT-PCR)

Veterinary Immunology and Immunopathology, 2023
Canine pemphigus foliaceus (PF) is a common autoimmune skin disease characterized by autoantibodies binding to epithelial adhesion molecules resulting inflammatory response. The immune network of cytokine and chemokine abnormalities that characterize the immune response in canine PF are poorly explored.
Haley Starr, Elizabeth Howerth, Robert Gogal Jr, Jamie Barber, Renato Leon, Amanda Blubaugh, Frane Banovic   +6 more
openaire   +2 more sources

A multiplex qRT-PCR assay for detection of Influenza A and H5 subtype targeting new SNPs present in high pathogenicity avian influenza Canadian 2022 outbreak strains

2023
Abstract H5N1 is a highly pathogenic avian Influenza A subtype that has been known to also infect mammalian hosts and presents a potential public health risk. To address and mitigate the affects of new SNPs, found in recent Canadian outbreaks, on diagnostic detection we developed two qRT-PCR assays by modifying current probes to match ...
Tracy D. Lee, Frankie Tsang, Kathleen Kolehmainen, Natalie A. Prystajecky, Agatha N. Jassem, John R. Tyson   +5 more
openaire   +1 more source

Efficient assessment of the utility of immortalized Fa2N-4 cells for cytochrome P450 (CYP) induction studies using multiplex quantitative reverse transcriptase-polymerase chain reaction (qRT-PCR) and substrate cassette methodologies

Xenobiotica, 2008
Induction of cytochrome P450 (CYP) 1A2, CYP2B6, and CYP3A4 by 22 prototypical inducers was evaluated in the Fa2N-4 immortalized human hepatic cell line. To facilitate this a duplex one-step quantitative reverse transcriptase-polymerase chain reaction (qRT-PCR) assay for CYP1A2 and CYP3A4 and a substrate cassette allowing simultaneous monitoring of ...
J R, Kenny, L, Chen, D F, McGinnity, K, Grime, K M, Shakesheff, B, Thomson, R, Riley   +6 more
openaire   +2 more sources

Development and optimization of a multiplex qRT-PCR assay for Influenza A detection and subtyping using double-quencher TaqMan probes

Annals of the International Symposium on Immunobiologicals
Priscilla Guimarães, Pedro Cardoso, Barbara Nogueira, Élida Brandão, Beatriz Barreto, Fernando Motta, Monica Arruda, Patrícia Baptista   +7 more
openaire   +1 more source

AN ELECTROMAGNETICALLY-DRIVEN PORTABLE MICROFLUIDIC SYSTEM FOR MULTIPLEX QRT-PCR DIAGNOSIS OF SARS-COV-2 AND INFLUENZA A/B VIRUSES

2024 MicroTAS Book of Abstracts
Ko-Hua Lin, Chih-Hung Wang, Ying-Jun Lin, Wen-Yen Huang, Yan-Shen Shan, Huey-Pin Tsai, Gwo-Bin Lee   +6 more
openaire   +1 more source

Detection and Profiling of Diarrheic Marine Biotoxins in Shellfish by mRNA Analysis of Exposed Caco-2 Cells Using qRT-PCR and Multiplex Magnetic Bead-Based Assays

ALTEX: Alternatives To Animal Experimentation, 2018
Toine F H Bovee
exaly  

Design of a Multiplex Quantitative Reverse Transcription-PCR System to Simultaneously Detect 16 Pathogens Associated With Bovine Respiratory and Enteric Diseases

Journal of Applied Microbiology, 2020
Tohru Suzuki
exaly  

Validation of a Multiplex Qrt-Pcr Assay for the Detection of Rsv, Influenza Virus A/B and Sars-Cov-2

ALIHAN BULGURCU, A. Arzu Sayıner
openaire   +1 more source

Multiplex, Quantitative, Reverse Transcription PCR Detection of Influenza Viruses Using Droplet Microfluidic Technology

Micromachines, 2015
Raymond Tellier, Kanti Pabbaraju, Anita Wong   +2 more
exaly  

Identification of Genes That Exhibit Changes in Expression on the 8p Chromosomal Arm by the Systematic Multiplex RT-PCR (SM RT-PCR) and DNA Microarray Hybridization Methods.

Gene Expression, 2008
Miyako Yamamoto, Fumiichiro Yamamoto
exaly