Results 101 to 110 of about 18,173,972 (253)

Simultaneous Screening of 24 Target Genes of Foodborne Pathogens in 35 Foodborne Outbreaks Using Multiplex Real-Time SYBR Green PCR Analysis

International Journal of Microbiology, 2010
A set of 8 multiplex real-time SYBR Green PCR (SG-PCR) assays including 3 target primers and an internal amplification control (IAC) primer was simultaneously evaluated in 3 h or less with regard to detection of 24 target genes of 23 foodborne pathogens ...
Hiroshi Fukushima, Jun Kawase, Yoshiki Etoh, Kumiko Sugama, Shunshuke Yashiro, Natsuko Iida, Keiji Yamaguchi   +6 more
doaj   +1 more source

Multidimensional and Multifunctional Laser‐Induced Graphene (LIG) for Point‐of‐Care and Wearable Biosensing, Theranostics, and Bioactive Interfaces Toward Personalized Healthcare and Regenerative Medicine

Advanced Science, EarlyView.
Multidimensional laser‐induced graphene (LIG) spanning from 0D to 3D architectures is comprehensively reviewed for multifunctional biomedical platforms, including biosensing, theranostics, and bioactive interface applications, which highlights its potentials for point‐of‐care diagnostics, wearable health monitoring, smart drug delivery, and tissue ...
Li Zhang, Yurou Yuan, Xiaohong Ding, Wing Cheung Mak   +3 more
wiley   +1 more source

A Machine Vision‐Guided Microphysiological Platform With Automated Microfluidics Enables Longitudinal Biomarker Monitoring and Emulation of Translationally Relevant Exposure Scenarios

Advanced Science, EarlyView.
A pneumatically actuated multi‐tissue microphysiological system is integrated with AI‐based machine vision and automatic sampling and replenishment systems. The platform allows for the emulation of translationally relevant long‐term pharmacokinetic exposure scenarios for multiple weeks while enabling longitudinal monitoring of response biomarkers ...
Jibbe Keulen, Yi Zhong, Laura Dangel, Sonia Youhanna, Reza Zandi Shafagh, Jinhye Ryu, Domnica‐Gabriela Gurau, Mathias Haag, Thomas E. Mürdter, Yingxin Liang, Iro Beligiani, Nicole Ziegler, Sabine Willems, Nayere Taebnia, Wouter van der Wijngaart, Volker M. Lauschke   +15 more
wiley   +1 more source

Nucleotide sequence of the primers and probes used in conventional multiplex PCR, multiplex real-time PCR.

, 2014
*Conventional multiplex PCR, according to McClure et al. (2006) [43].†Multiplex real-time PCR, according to McDonald et al. (2005) [35].
Julie S. Sherwood (567228), Valeria Velasco (567227), Catherine M. Logue (294873), Pedro P. Rojas-García (5659072)   +3 more
core   +1 more source

Bioengineered Exosome‐Loaded Immunomodulatory Bioadhesive Spray Reverses Liver Fibrosis and Metabolic Dysfunction That Triggers Beneficial Gut‐Liver Crosstalk in Chronic Fatty Liver Disease

Advanced Science, EarlyView.
Umbilical cord stem cell‐derived exosomes encapsulated in an immunomodulatory bioadhesive hydrogel spray enable localized therapy, sustained vesicle delivery, macrophage polarization, suppress systemic inflammation, and mitigate glucose and insulin homeostasis impairments in MAFLD.
Triya Saha, Ayushi Mairal, Shreya Mehrotra, Shiv K. Sarin, Ashok Kumar   +4 more
wiley   +1 more source

〈Review〉Real-time PCR and multiplex real-time PCR for ocular infections by human herpes viruses [PDF]

, 2015
publisherCurrently, there are 9 human herpes viruses known that remain latent at various sites of the human body, and some of them could become reactivated and trigger various diseases.
ヒガキ, シロウ, イタハシ, モトキ, Higaki, Shiro, Itahashi, Motoki, Shimomura, Yoshikazu, シモムラ, ヨシカズ   +5 more
core   +1 more source

Reproducibility of the multiplex real-time PCR assays.

, 2016
Reproducibility of the multiplex real-time PCR assays.
Yongyu Rui (3156081), Qiu Yang (572481)
core   +1 more source

Systematic Position Mapping of Split CRISPR‐Cas12a Activators Enables Highly Sensitive miRNA Detection and Cancer Cell Stratification

Advanced Science, EarlyView.
ABSTRACT Amplification‐free Cas12a diagnostics with split crRNA enable rapid and programmable target recognition, yet insufficient understanding of DNA activator architecture prevents predictable control over trans‐cleavage activity and sensitivity. Here we systematically map over 200 split DNA activator configurations by introducing nicks at every ...
Xiaoyan Tang, Zhe Li, Yuning Lu, Miao Ma, Zijian Mo, Jiajun Ke, Xinyu Luan, Tiangang Luan, Junqiu Zhai   +8 more
wiley   +1 more source

A highly efficient and accurate method of detecting and subtyping Influenza A pdm H1N1 and H3N2 viruses with newly emerging mutations in the matrix gene in Eastern Taiwan

PLoS ONE, 2023
The rapid identification of Influenza A virus and its variants, which cause severe respiratory diseases, is imperative to providing timely treatment and improving patient outcomes.
Hui-Hua Yang, I-Tsong Huang, Ren-Chieh Wu, Li-Kuang Chen   +3 more
doaj  

A novel real-time PCR for Listeria monocytogenes that monitors analytical performance via an internal amplification control [PDF]

, 2005
We describe a novel quantitative real-time (Q)-PCR assay for Listeria monocytogenes based on the coamplification of a target hly gene fragment and an internal amplification control (IAC). The IAC is a chimeric double-stranded DNA containing a fragment of
Vázquez-Boland, JA, Pla, Maria, Monzó, HJ, Rodríguez-Lázaro, AD, Scortti, MM, Rodríguez-Lázaro, David, Monzó, Hector J, Pla, M, Scortti, Mariela, Vazquez-Boland, Jose A   +9 more
core