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Diferenciação de micobactérias por PCR multiplex Differentiation of micobacteria by multiplex PCR

Revista da Sociedade Brasileira de Medicina Tropical, 2009
O trabalho visou à otimização de um método baseado na reação em cadeia da polimerase multiplex - para diferenciação de micobactérias de interesse para a saúde pública.
Diogo da Rocha Poroca, Andrea Santos Lima, Juliana Falcão de Araújo Lima, Heidi Lacerda Alves da Cruz, Rosana de Albuquerque Montenegro, Fábio Lopes de Melo, Haiana Charifker Schindler, Lílian Maria Lapa Montenegro   +7 more
doaj   +3 more sources

Rapid multiplex PCR for respiratory viruses reduces time to result and improves clinical care: Results of a systematic review and meta-analysis

Journal of Infection, 2023
OBJECTIVES The clinical impact of rapid sample-to-answer 'syndromic' multiplex polymerase chain reaction (PCR) testing for respiratory viruses is not clearly established.
Jennifer Uyei, Tristan T Timbrook
exaly   +2 more sources

Multiplex-PCR for diagnosis of bacterial meningitis [PDF]

Brazilian Journal of Microbiology, 2019
Considering the great lethality and sequels caused by meningitis, rapid diagnosis and prompt treatment initiation have a great impact on patient outcome. Here, we developed a multiplex-PCR for simultaneous detection of the four most prevalent bacterial pathogens directly in CSF samples. The multiplex-PCR was designed to detect the following genes: fbsA
R. Albuquerque, A. C. R. Moreno, S. R. Santos, S. B. Ragazzi, M. Martinez   +4 more
semanticscholar   +3 more sources

Identification and Discrimination of Salmonella enterica Serovar Gallinarum Biovars Pullorum and Gallinarum Based on a One-Step Multiplex PCR Assay

Frontiers in Microbiology, 2018
Salmonella enterica serovar Gallinarum biovars Pullorum (S. Pullorum) and Gallinarum (S. Gallinarum) can result in pullorum disease and fowl typhoid in avian species, respectively, and cause considerable economic losses in poultry in many developing ...
Dan Xiong, Dan Xiong, Dan Xiong, Dan Xiong, Li Song, Li Song, Li Song, Li Song, Zhiming Pan, Zhiming Pan, Zhiming Pan, Zhiming Pan, Xinan Jiao, Xinan Jiao, Xinan Jiao, Xinan Jiao   +15 more
doaj   +2 more sources

Multiplex-PCR [PDF]

Springer Reference Medizin, 2019
J. Arnemann
semanticscholar   +3 more sources

Designing highly multiplex PCR primer sets with Simulated Annealing Design using Dimer Likelihood Estimation (SADDLE)

Nature Communications, 2021
One major challenge in the design of highly multiplexed PCR primer sets is the large number of potential primer dimer species that grows quadratically with the number of primers to be designed.
Nina G. Xie, M. Wang, P. Song, Shiqi Mao, Yifan Wang, Yuxia Yang, Junfeng Luo, Shengxiang Ren, David Yu Zhang   +8 more
semanticscholar   +1 more source

Outcomes and clinical relevance of stool multiplex bacterial polymerase chain reaction in patients with acute diarrhea: single center experience [PDF]

The Korean Journal of Internal Medicine, 2020
Background/Aims Diagnostic stool multiplex polymerase chain reaction (PCR) testing has attracted considerable interest, because of its high sensitivity, short turnaround time, and ability to detect multiple organisms simultaneously.
Won Gun Kwack, Yun Jeong Lim, Ki Hwan Kwon, Jae Woo Chung, Jin Young Oh   +4 more
doaj   +1 more source

Usefulness of Stool Multiplex Polymerase Chain Reaction Assays in Patients with Acute Diarrhea

The Korean Journal of Gastroenterology, 2022
Background/Aims: There is a recent increase in the use of stool multiplex PCR assay-based diagnostic tests in patients with acute diarrhea. We used multiplex PCR assays to analyze the distribution of diarrhea-causing bacteria and viruses, as well as the ...
Seo Hyun Kim, You Sun Kim, Seung Hyuk Kim, Won Eui Yoon, Hee Jun Myung, Jeong Seop Moon, Dong Hee Whang   +6 more
doaj   +1 more source

Direct Detection and Identification of the Most Common Bacteria and Fungi Causing Otitis Externa by a Stepwise Multiplex PCR

Frontiers in Cellular and Infection Microbiology, 2021
Background Considering the importance of differential diagnosis of infectious otitis externa (OE), a stepwise PCR-based assay using universal and genus- or species-specific primers for the detection/identification of the most prevalent bacterial and ...
Shima Aboutalebian, K. Ahmadikia, H. Fakhim, J. Chabavizadeh, A. Okhovat, M. Nikaeen, H. Mirhendi   +6 more
semanticscholar   +1 more source

Diagnostic accuracy of multiplex polymerase chain reaction on tissue biopsies in periprosthetic joint infections

Scientific Reports, 2021
The diagnosis and treatment of periprosthetic joint infection (PJI) currently relies on cultures, which are time-consuming and often fail. Multiplex PCR assays promise reliable and prompt results, but have been heterogeneously evaluated.
Igor Lazic, Susanne Feihl, Peter M. Prodinger, Ingo J. Banke, Andrej Trampuz, Rüdiger von Eisenhart-Rothe, Christian Suren   +6 more
doaj   +1 more source