Results 21 to 30 of about 957,735 (260)

Multiplex PCR: Critical Parameters and Step-by-Step Protocol

BioTechniques, 1997
By simultaneously amplifying more than one locus in the same reaction, multiplex PCR is becoming a rapid and convenient screening assay in both the clinical and the research laboratory.
O. Henegariu, N.A. Heerema, S.R. Dlouhy, G.H. Vance, P.H. Vogt   +4 more
doaj   +1 more source

Development of a Multiplex Crystal Digital RT-PCR for Differential Detection of Classical, Highly Pathogenic, and NADC30-like Porcine Reproductive and Respiratory Syndrome Virus

Animals, 2023
Porcine reproductive and respiratory syndrome virus (PRRSV) type 1 (European genotype) and PRRSV type 2 (North American genotype) are prevalent all over the world.
Feng Long, Yating Chen, Kaichuang Shi, Yanwen Yin, Shuping Feng, Hongbin Si   +5 more
doaj   +1 more source

Development of a multiplex DNA-based traceability tool for crop plant materials [PDF]

, 2011
The authenticity of food is of increasing importance for producers, retailers and consumers. All groups benefit from the correct labelling of the contents of food products.
Theo W. Prins, Jeroen P. van Dijk, van Dijk, J.P., van Hoef, A.M.A., Kok, E.J., Ralf Seyfarth, Marleen M. Voorhuijzen, Seyfarth, R., A. M. Angeline Van Hoef, Hoef, A.M.A., van, Dijk, J.P., van, Voorhuijzen, M.M., Prins, T.W., Esther J. Kok   +13 more
core   +1 more source

Optimizations of high throughput multiplex polymerase chain reaction with simple sequence repeat markers for genotyping of common walnut populations (Juglans regia L.)

Silva Fennica, 2016
Multiplex polymerase chain reaction (PCR) allows amplification of two or more pair of primers in parallel for amplification of multiple target sequences in a single reaction tube.
Nevenka Ćelepirović, Monika Karija Vlahović, Aikaterini Dounavi, Mladen Ivanković   +3 more
doaj   +1 more source

primerJinn: a tool for rationally designing multiplex PCR primer sets for amplicon sequencing and performing in silico PCR

BMC Bioinformatics, 2023
Background Multiplex PCR amplifies numerous targets in a single tube reaction and is essential in molecular biology and clinical diagnostics. One of its most important applications is in the targeted sequencing of pathogens.
Jason D. Limberis, John Z. Metcalfe
doaj   +1 more source

PCR múltiplex para el diagnóstico de microorganismos atípicos transmitidos sexualmente

Perinatología y Reproducción Humana, 2023
Antecedentes: Las infecciones de transmisión sexual son un problema de salud pública mundial. El análisis rutinario incluye solo pruebas microbiológicas y serológicas para el diagnóstico de patógenos.
Marcos R. Escobedo-Guerra, Marcela López-Hurtado, Rodrigo Gutiérrez-Trujillo, Abraham D. Bustos-López, Fernando M. Guerra-Infante   +4 more
doaj   +1 more source

Analysis of a novel multiplex polymerase chain reaction assay as a sensitive tool for the diagnosis of indeterminate and tuberculoid forms of leprosy

International Journal of Mycobacteriology, 2017
Objective/Background: Clinical diagnosis of indeterminate and tuberculoid leprosy is often difficult due to limited and confounding signs and symptoms. In the current study, we evaluated the utility of new multiplex polymerase chain reaction (PCR) using
V Sundeep Chaitanya, Luz Cuello, Madhusmita Das, A Sudharsan, Pavithrra Ganesan, K Kanmani, Lakshmi Rajan, Mannam Ebenezer   +7 more
doaj   +1 more source

Multiplex PCR assay based on the citE2 gene and intergenic sequence for the rapid detection of Salmonella Pullorum in chickens

Poultry Science, 2022
: Salmonella is one of the most common Gram-negative pathogens and seriously threatens chicken farms and food safety. This study aimed to establish a multiplex polymerase chain reaction (PCR) approach for the identification of different Salmonella ...
Zhike Liu, Yan Yu, Tetiana Fotina, Roman Petrov, Zhanna Klishchova, Anatoliy Fotin, Jinyou Ma   +6 more
doaj   +1 more source

Evaluation of multiplex ligation-dependent probe amplification analysis versus multiplex polymerase chain reaction assays in the detection of dystrophin gene rearrangements in an Iranian population subset

Advanced Biomedical Research, 2014
Background: The Duchenne muscular dystrophy (DMD) gene is located in the short arm of the X chromosome (Xp21). It spans 2.4 Mb of the human genomic DNA and is composed of 79 exons.
Nayereh Nouri, Esmat Fazel-Najafabadi, Mansoor Salehi, Majid Hosseinzadeh, Mahdieh Behnam, Mohammad Reza Ghazavi, Maryam Sedghi   +6 more
doaj   +1 more source

Primer1: Primer design Web service for Tetra-Primer ARMS-PCR [PDF]

, 2012
Tetra-primer ARMS-PCR is used extensively as a low cost, single PCR assay requiring no post-PCR manipulation. The design of successful primers depends on a number of variables such as melting temperatures, GC content, complementarity and selection of ...
Ke, Xiayi, Collins, Andrew
core   +1 more source