Results 21 to 30 of about 1,719,427 (359)

Detection and identification of mycobacteria in sputum from suspected tuberculosis patients

BMC Research Notes, 2010
Background Detection of Tuberculosis agent like nontuberculous mycobacteria (NTM) species by culture and microscopic methods remains difficult and time consuming.
Yadi, Masjudi, Tandirogang Nataniel, Sultan Andi, Hatta Mochammad   +4 more
doaj   +1 more source

Multiplex PCR for identifying mycobacterial isolates. [PDF]

Journal of Clinical Pathology, 1995
AIMS--To develop a multiplex polymerase chain reaction (PCR) method to facilitate identification of mycobacterial isolates. METHODS--Type strains of 14 species of mycobacteria and 56 clinical isolates were lysed by boiling in TE Triton. The lysate (5 microliters) was used directly in a PCR reaction incorporating three pairs of PCR primers expected to ...
Cormican, M, Glennon, M, Riain, U N, Flynn, J   +3 more
openaire   +3 more sources

MegaPlex PCR: a strategy for multiplex amplification [PDF]

Nature Methods, 2007
'MegaPlex PCR' is a robust technology for highly multiplexed amplification of specific DNA sequences. It uses target-specific pairs of PCR primers that are physically separated by surface immobilization. Initial surface-based amplification cycles are then coupled to efficient solution-phase PCR using one common primer pair.
Meuzelaar, Linda Strömqvist, Lancaster, Owen, Pasche, J. Paul, Kopal, Guido, Brookes, Anthony J.   +4 more
openaire   +2 more sources

Multiplex PCR for detection of plasmid-mediated colistin resistance determinants, mcr-1, mcr-2, mcr-3, mcr-4 and mcr-5 for surveillance purposes

Euro surveillance : bulletin Europeen sur les maladies transmissibles = European communicable disease bulletin, 2018
Background and aim Plasmid-mediated colistin resistance mechanisms have been identified worldwide in the past years. A multiplex polymerase chain reaction (PCR) protocol for detection of all currently known transferable colistin resistance genes (mcr-1 ...
A. R. Rebelo, Valeria Bortolaia, J. Kjeldgaard, S. Pedersen, P. Leekitcharoenphon, I. M. Hansen, B. Guerra, B. Malorny, M. Borowiak, J. Hammerl, A. Battisti, A. Franco, Patricia Alba, A. Perrin-Guyomard, S. Granier, Cristina De Frutos Escobar, S. Malhotra-Kumar, L. Villa, A. Carattoli, R. Hendriksen   +19 more
semanticscholar   +1 more source

Comparison and transfer testing of multiplex ligation detection methods for GM plants [PDF]

, 2012
Background With the increasing number of GMOs on the global market the maintenance of European GMO regulations is becoming more complex. For the analysis of a single food or feed sample it is necessary to assess the sample for the presence of many GMO ...
van Dijk Jeroen P, van Hoef, A.M.A., Beenen Henriek G, van Dijk, Jeroen P., Voorhuijzen, Marleen, Marleen M Voorhuijzen, Theo W Prins, Hoef, A.M.A., van, Gruden, K., Jeroen P van Dijk, Kristina Gruden, Prins Theo W, Gabriella Ujhelyi, Voorhuijzen, M.M., Kok Esther J, Ujhelyi, Gabriella, Gruden Kristina, Morisset, D., Gruden, Kristina, AM Angeline Van Hoef, Morisset, Dany, van Dijk, J.P., Kok, E.J., Prins, Theo W., Esther J Kok, Henriek G Beenen, Beenen, H.G., Van Hoef, Angeline, Morisset Dany, Ujhelyi, G., Dijk, J.P., van, Dany Morisset, Angeline Van Hoef AM, Prins, T.W., Ujhelyi Gabriella, Kok, Esther, Voorhuijzen Marleen M, Beenen, Henriek G.   +37 more
core   +1 more source

Clinical use of multiplex-PCR for the diagnosis of acute bacterial meningitis

Journal of Family Medicine and Primary Care, 2022
Background and Objectives: Prompt and accurate diagnosis of acute bacterial meningitis (ABM) is critical for patient management. We designed and evaluated two sets of multiplex-PCR assays for the simultaneous detection of six major etiologies of ABM i.e.,
Nupur Sharma, Hitender Gautam, Sonu Tyagi, Shahid Raza, Sarita Mohapatra, Seema Sood, Benu Dhawan, Arti Kapil, Bimal K Das   +8 more
doaj   +1 more source

Fundamentals of multiplexing with digital PCR

Biomolecular Detection and Quantification, 2016
Over the past decade numerous publications have demonstrated how digital PCR (dPCR) enables precise and sensitive quantification of nucleic acids in a wide range of applications in both healthcare and environmental analysis. This has occurred in parallel with the advances in partitioning fluidics that enable a reaction to be subdivided into an ...
Whale, Alexandra S., Huggett, Jim F., Tzonev, Svilen   +2 more
openaire   +2 more sources

Development of multiplex PCR for rapid detection of metallo-β-lactamase genes in clinical isolates of Acinetobacter baumannii

Iranian Journal of Microbiology, 2020
Background and Objectives: Acinetobacter baumannii has been known as a major pathogen causing nosocomial infections. The aim of this study was to develop multiplex PCR for rapid and simultaneous detection of metallo-β-lactamase (MBL) genes in clinical ...
Reza Ranjbar, Shahin Zayeri, Amir Mirzaie   +2 more
doaj   +1 more source

Molecular diagnosis of the human immunodeficiency, Hepatitis B and C viruses among blood donors in Lomé (Togo) by multiplex real time PCR

The Pan African Medical Journal, 2016
This study aimed to compare the sensitivity of multiplex PCR to ELISA technique in the instantaneous detection of HBV, HCV and HIVin blood samples from donors of the National blood Transfusion Centre in Togo.
Maleki Assih, Lochina Feteke, Cyrille Bisseye, Djeneba Ouermi, Florencia Djigma, Simplice Damintoti Karou, Jacques Simpore   +6 more
doaj   +1 more source

Fragment Size Difference between Multiplex and Singleplex PCR Products and Their Practical Implications

BioTechniques, 2000
By simultaneously amplifying several loci in the same reaction, multiplex PCR has been used in gene mapping and DNA typing with polymorphic short tandem repeat loci.
H.-W. Deng, Y. Zhou, R.R. Recker, M.L. Johnson, J. Li   +4 more
doaj   +1 more source