Impact of multiplex polymerase chain reaction testing in patients with bacteremia [PDF]
Microbiology SpectrumRapid identification of pathogens in bacteremia is critical for optimizing antimicrobial therapy. The FilmArray blood culture identification 2 (BCID2) panel enables multiplex PCR-based detection of pathogens and resistance genes.
Daisuke Kitagawa +16 more
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Multiplex polymerase chain reaction for pathogen identification and antibiotic stewardship in human immunodeficiency virus-associated diarrhea: A retrospective study and systematic review [PDF]
SAGE Open MedicineBackground: Diarrhea causes significant morbidity among people living with human immunodeficiency virus. Compared to conventional diagnostic tests, multiplex polymerase chain reaction gastrointestinal panels provide faster and more accurate pathogen ...
Santiago Gómez-Jordan +6 more
doaj +2 more sources
Diagnostic Accuracy of Multiplex Polymerase Chain Reaction in Early Onset Neonatal Sepsis [PDF]
Children, 2023 Early onset neonatal sepsis is a significant contributor to neonatal morbidity and mortality. Although blood cultures remain the diagnostic gold standard, they detect pathogens in only a minority of suspected cases.
Anja Stein +3 more
doaj +2 more sources
Multiplex polymerase chain reaction detection of enteropathogens in sewage in Norway [PDF]
Acta Veterinaria Scandinavica, 2019 The primary objective of this small-scale study was to investigate the occurrence of enteropathogens in sewage (municipal wastewater) in Norway using the commercially available FilmArray® multiplex polymerase chain reaction (PCR) system with the ...
Øyvind Ørmen +2 more
doaj +3 more sources
Rapid identification of bacteria using a multiplex polymerase chain reaction system for acute abdominal infections [PDF]
Frontiers in Microbiology, 2023 Yoshihisa Saida +2 more
exaly +2 more sources
Pakistan Armed Forces Medical Journal, 2021 Objective: To detect Neisseria gonorrhoeae from the urine of male patients reporting with active urethral discharge using multiplex polymerase chain reaction (PCR).
Hafsa Waseem +5 more
doaj +1 more source
Thermally multiplexed polymerase chain reaction [PDF]
Biomicrofluidics, 2015 Amplification of multiple unique genetic targets using the polymerase chain reaction (PCR) is commonly required in molecular biology laboratories. Such reactions are typically performed either serially or by multiplex PCR. Serial reactions are time consuming, and multiplex PCR, while powerful and widely used, can be prone to amplification bias, PCR ...
Christopher R. Phaneuf +10 more
openaire +2 more sources
A simple, rapid and economic method for detecting multidrug-resistant tuberculosis
Brazilian Journal of Infectious Diseases, 2013 Objective: To evaluate multiplex allele specific polymerase chain reaction as a rapid molecular tool for detecting multidrug-resistant tuberculosis. Methods: Based on drug susceptibility testing, 103 isolates were multidrug-resistant tuberculosis and 45 ...
Xia Wang +5 more
doaj +3 more sources
Multiplex polymerase chain reaction: A practical approach [PDF]
Journal of Clinical Laboratory Analysis, 2002 AbstractConsiderable time and effort can be saved by simultaneously amplifying multiple sequences in a single reaction, a process referred to as multiplex polymerase chain reaction (PCR). Multiplex PCR requires that primers lead to amplification of unique regions of DNA, both in individual pairs and in combinations of many primers, under a single set ...
P, Markoulatos, N, Siafakas, M, Moncany
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Multiplex polymerase chain reaction (M-PCR) for bacterial vaginosis detection
Vietnam Journal of Science, Technology and Engineering, 2022 Bacterial vaginosis (BV) is one of the most common vaginal infections in women of reproductive age. If not treated promptly, the disease can lead to serious complications affecting the fertility and long-term health of women.
Tien Sang Trieu +7 more
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