Results 11 to 20 of about 8,966 (157)

Multiplex polymerase chain reaction detection of enteropathogens in sewage in Norway [PDF]

Acta Veterinaria Scandinavica, 2019
The primary objective of this small-scale study was to investigate the occurrence of enteropathogens in sewage (municipal wastewater) in Norway using the commercially available FilmArray® multiplex polymerase chain reaction (PCR) system with the ...
Øyvind Ørmen, Kristian Aalberg, Elisabeth Henie Madslien   +2 more
doaj   +3 more sources

Impact of multiplex polymerase chain reaction testing in patients with bacteremia

Microbiology Spectrum
Rapid identification of pathogens in bacteremia is critical for optimizing antimicrobial therapy. The FilmArray blood culture identification 2 (BCID2) panel enables multiplex PCR-based detection of pathogens and resistance genes.
Daisuke Kitagawa, Taito Kitano, Takehito Kasamatsu, Naoyuki Shiraishi, Mai Yasuda, Mai Okada, Soma Suzuki, Madoka Sekine, Ryo Yamanishi, Ayu Mukai, Ritsuki Uejima, Yuki Suzuki, Akiyo Nakano, Ryuichi Nakano, Hisakazu Yano, Fumihiko Nakamura, Koichi Maeda   +16 more
doaj   +4 more sources

Role of multiplex polymerase chain reaction in diagnosing tubercular meningitis

Journal of Laboratory Physicians, 2017
Tuberculous meningitis (TBM) is one of the most serious manifestations of extrapulmonary tuberculosis. Timely and accurate diagnosis provides a favorable prognosis in patients with TBM.
Vishnu Prasad Shenoy, Anupam Berwal, Kiran Chawla, Shashidhar Vishwanath   +3 more
core   +3 more sources

Detection of bovine pestiviruses by a multiplex real-time polymerase chain reaction

Вопросы вирусологии, 2020
Introduction. Pestiviruses are the cause of reproductive problems, diseases of the gastrointestinal and respiratory tracts of animals. Three species are important for cattle: Pestivirus A, B, and H.
Aleksey V. Nefedchenko, Svetlana V. Koteneva, Tatyana I. Glotova, Alexander G. Glotov   +3 more
doaj   +4 more sources

Thermally multiplexed polymerase chain reaction [PDF]

Biomicrofluidics, 2015
Amplification of multiple unique genetic targets using the polymerase chain reaction (PCR) is commonly required in molecular biology laboratories. Such reactions are typically performed either serially or by multiplex PCR. Serial reactions are time consuming, and multiplex PCR, while powerful and widely used, can be prone to amplification bias, PCR ...
Christopher R. Phaneuf, Nikita Pak, D. Curtis Saunders, Gregory L. Holst, Joav Birjiniuk, Nikita Nagpal, Stephen Culpepper, Emily Popler, Andi L. Shane, Robert Jerris, Craig R. Forest   +10 more
openaire   +2 more sources

Multiplex polymerase chain reaction: A practical approach [PDF]

Journal of Clinical Laboratory Analysis, 2002
AbstractConsiderable time and effort can be saved by simultaneously amplifying multiple sequences in a single reaction, a process referred to as multiplex polymerase chain reaction (PCR). Multiplex PCR requires that primers lead to amplification of unique regions of DNA, both in individual pairs and in combinations of many primers, under a single set ...
P, Markoulatos, N, Siafakas, M, Moncany
openaire   +2 more sources

Identification of Dermatophytes Using Multiplex Polymerase Chain Reaction [PDF]

Annals of Dermatology, 2011
Multiplex polymerase chain reaction (PCR) allows more than two target DNA molecules to be amplified with more than two primers. This method is also useful for detecting various other organisms simultaneously within a single test tube, and the scope of its use has been expanding widely in the field of clinical microbiology in recent years.To assess the ...
Kim, Ji Young, Choe, Yong Beom, Ahn, Kyu Joong, Lee, Yang Won   +3 more
openaire   +2 more sources

Multiplex Polymerase Chain Reaction for diagnosis of gastrointestinal tuberculosis [PDF]

JGH Open, 2018
Background and AimTo evaluate the role of multiplex polymerase chain reaction (PCR) for diagnosis of gastrointestinal tuberculosis (GITB).MethodsThis was a prospective observational study conducted from July 2015 to November 2016 at a tertiary care teaching institution in north India.
Sarthak Malik, Kusum Sharma, Kim Vaiphei, Narendra Dhaka, Neha Berry, Pankaj Gupta, Megha Sharma, Bipadabhanjan Mallick, Rakesh Kochhar, Saroj K Sinha   +9 more
openaire   +2 more sources

Optimised padlock probe ligation and microarray detection of multiple (non-authorised) GMOs in a single reaction [PDF]

, 2008
Background To maintain EU GMO regulations, producers of new GM crop varieties need to supply an event-specific method for the new variety. As a result methods are nowadays available for EU-authorised genetically modified organisms (GMOs), but only to a ...
Aarts, H.J.M., AM Angeline Van Hoef, van Dijk, J.P., van Hoef, A.M.A., Kok, E.J., Esther J Kok, Henriek G Beenen, Schoen, C.D., Beenen, H.G., Cor D Schoen, Henk JM Aarts, Marleen M Voorhuijzen, Hoef, A.M.A., van, Jeroen P van Dijk, Theo W Prins, Dijk, J.P., van, Prins, T.W., Voorhuijzen, M.M.   +17 more
core   +1 more source

Multiplex PCR-based titration (MPBT) assay for determination of infectious titers of the three Sabin strains of live poliovirus vaccine

Virology Journal, 2019
Background Conventional assays to titrate polioviruses usually test serial dilutions inoculated into replicate cell cultures to determine a 50% cytopathic endpoint, a process that is both time-consuming and laborious.
Hasmik Manukyan, Elvira Rodionova, Tatiana Zagorodnyaya, Tsai-Lien Lin, Konstantin Chumakov, Majid Laassri   +5 more
doaj   +1 more source