Results 11 to 20 of about 65,416 (260)

Identification of Dermatophytes Using Multiplex Polymerase Chain Reaction [PDF]

Annals of Dermatology, 2011
Multiplex polymerase chain reaction (PCR) allows more than two target DNA molecules to be amplified with more than two primers. This method is also useful for detecting various other organisms simultaneously within a single test tube, and the scope of its use has been expanding widely in the field of clinical microbiology in recent years.To assess the ...
Kim, Ji Young, Choe, Yong Beom, Ahn, Kyu Joong, Lee, Yang Won   +3 more
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Enhanced detection and serotyping of using multiplex polymerase chain reaction [PDF]

Korean Journal of Pediatrics, 2012
PurposeMethods for quick and reliable detection of Streptococcus pneumoniae are needed for the diagnosis of pneumococcal disease and vaccine studies. This study aimed to show that sequential multiplex polymerase chain reaction (PCR) is more efficient ...
Jong Gyun Ahn, Seong Yeol Choi, Dong Soo Kim, Ki Hwan Kim   +3 more
doaj   +1 more source

Case Report: Identification of Maternal Low-Level Mosaicism in the Dystrophin Gene by Droplet Digital Polymerase Chain Reaction

Frontiers in Genetics, 2021
Germline mosaicism should be suspected when the same de novo mutations are identified in a second pregnancy with asymptomatic parents. Our study aims to find a feasible approach to reveal the existence of germline mosaicism.
Pengzhen Jin, Pengzhen Jin, Xiaoyang Gao, Xiaoyang Gao, Miaomiao Wang, Miaomiao Wang, Yeqing Qian, Yeqing Qian, Jingjin Yang, Jingjin Yang, Yanmei Yang, Yanmei Yang, Yuqing Xu, Yuqing Xu, Yanfei Xu, Yanfei Xu, Minyue Dong, Minyue Dong   +17 more
doaj   +1 more source

Multiplex Polymerase Chain Reaction for diagnosis of gastrointestinal tuberculosis [PDF]

JGH Open, 2018
Background and AimTo evaluate the role of multiplex polymerase chain reaction (PCR) for diagnosis of gastrointestinal tuberculosis (GITB).MethodsThis was a prospective observational study conducted from July 2015 to November 2016 at a tertiary care teaching institution in north India.
Sarthak Malik, Kusum Sharma, Kim Vaiphei, Narendra Dhaka, Neha Berry, Pankaj Gupta, Megha Sharma, Bipadabhanjan Mallick, Rakesh Kochhar, Saroj K Sinha   +9 more
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Phenotypic and genotypic characterization of locally isolated Salmonella strains used in preparation of Salmonella antigens in Egypt [PDF]

Veterinary World, 2016
Aim: This work was conducted to study the phenotypic and genotypic characterization of locally isolated Salmonella strains (Salmonella Pullorum, Salmonella Enteritidis, and Salmonella Typhimurium) from poultry used in the preparation of Salmonella ...
Hazem Mohammed Ibrahim, Dalia Ahmed Mohammed Abd El-moaty, Hanan Ali Ahmed, Mona Ibrahim El-Enbaawy   +3 more
doaj   +1 more source

Interspecies comparison of probiotics isolated from different animals [PDF]

Veterinary World, 2018
Aim: The aim of the current study was to isolate and identify naturally occurring probiotic Lactobacillus species in different animals with the different environmental background including fish, and farm animals to investigate interspecies differences in
Amr M. Abdou, Riham H. Hedia, Shimaa T. Omara, Mohamed Abd El-Fatah Mahmoud, Mai M. Kandil, M. A. Bakry   +5 more
doaj   +1 more source

A search for Clostridium difficile ribotypes 027 and 078 in Brazil

Brazilian Journal of Infectious Diseases, 2014
Toxigenic strains of Clostridium difficile may be disseminating. Here we prospectively screened patients with nosocomial diarrhoea in two hospitals in Brazil. To identify C.
Alexandre de Almeida Monteiro, Renata Neto Pires, Søren Persson, Edison Moraes Rodrigues Filho, Alessandro Comarú Pasqualotto   +4 more
doaj   +1 more source

Multiplex PCR-based titration (MPBT) assay for determination of infectious titers of the three Sabin strains of live poliovirus vaccine

Virology Journal, 2019
Background Conventional assays to titrate polioviruses usually test serial dilutions inoculated into replicate cell cultures to determine a 50% cytopathic endpoint, a process that is both time-consuming and laborious.
Hasmik Manukyan, Elvira Rodionova, Tatiana Zagorodnyaya, Tsai-Lien Lin, Konstantin Chumakov, Majid Laassri   +5 more
doaj   +1 more source

Results of Multiplex Polymerase Chain Reaction Assay to Identify Urethritis Pathogens

Journal of Urological Surgery, 2017
Objective: The purpose of this study was to evaluate the results of multiplex polymerase chain reaction (PCR) test applied to identify the pathogens in male patients who attended our urology clinic with a pre-diagnosis of urethritis related with sexual ...
Mehmet Sarıer, İbrahim Duman, Şafak Göktaş, Meltem Demir, Erdal Kukul   +4 more
doaj   +1 more source

Specific detection of Salmonella spp. by multiplex polymerase chain reaction [PDF]

Applied and Environmental Microbiology, 1993
Three sets of oligonucleotide primers were used in the polymerase chain reaction (PCR) assay to detect Salmonella species. phoP primers specific to the phoP/phoQ loci of coliform pathogenic bacteria such as Salmonella, Shigella, Escherichia coli, and Citrobacter species served as presumptive indicators of enteric bacteria.
J S, Way, K L, Josephson, S D, Pillai, M, Abbaszadegan, C P, Gerba, I L, Pepper   +5 more
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