Results 21 to 30 of about 65,416 (260)

OCCURRENCE OF CAMPYLOBACTER SPECIES IN CHICKENS BY MULTIPLEX POLYMERASE CHAIN REACTION [PDF]

Assiut Veterinary Medical Journal, 2017
The genus Campylobacter is one of great importance to public health because it includes several species that may cause diarrhea. Poultry and poultry products are known as important sources of human campylobacteriosis. 225 samples were collected from (75)
ASMAA GAHLAN YOUSEEF, A.I. IBRAHIM, AMAL SAYED, MONA SOBHY   +3 more
doaj   +1 more source

Detection of blaOXA-10 and blaOXA-48 Genes in Pseudomonas aeruginosa Clinical Isolates by Multiplex PCR

Journal of Medical Microbiology and Infectious Diseases, 2021
Introduction: The rapidly increasing extended-spectrum β-lactamase-producing Pseudomonas aeruginosa is a threat to health. This study aims to detect the rpoD gene and blaOXA-10 and blaOXA-48 genes in imipenem-resistant P.
Aghdas Bibi Hashemi, Mahboobeh Nakhaei Moghaddam, Mohammad Mahdi Forghanifard, Ehsan Yousefi   +3 more
doaj   +1 more source

Outcomes and clinical relevance of stool multiplex bacterial polymerase chain reaction in patients with acute diarrhea: single center experience [PDF]

The Korean Journal of Internal Medicine, 2020
Background/Aims Diagnostic stool multiplex polymerase chain reaction (PCR) testing has attracted considerable interest, because of its high sensitivity, short turnaround time, and ability to detect multiple organisms simultaneously.
Won Gun Kwack, Yun Jeong Lim, Ki Hwan Kwon, Jae Woo Chung, Jin Young Oh   +4 more
doaj   +1 more source

Detection of Microsatellite Instability by Fluorescence Multiplex Polymerase Chain Reaction [PDF]

The Journal of Molecular Diagnostics, 2000
We have created a clinical molecular diagnostic assay to test for microsatellite instability (MSI) at multiple loci simultaneously in paraffin-embedded surgical pathology colon resection specimens. This fluorescent multiplex polymerase chain reaction (PCR) assay analyzes the five primary microsatellite loci recommended at the 1997 National Cancer ...
K D, Berg, C L, Glaser, R E, Thompson, S R, Hamilton, C A, Griffin, J R, Eshleman   +5 more
openaire   +2 more sources

Brazilian ground beef authentication by multiplex polymerase chain reaction

Ciência Rural, 2018
: The aim of the present study was to assess the efficacy ofmultiplex PCR in detecting the adulterationof commercially available ground beefvia addition and/orsubstitution ofground buffalo meat. Experimentally adulterated ground beefsamples were prepared
Andrey Carlos do Sacramento de Oliveira, Silvia Cristina da Silva Pedroso, Diogo José Cardilli, Fábio Pereira Leivas Leite, Gabrielle Virgínia Lopes Ferreira, Andréia Silva da Silva, Talita Bandeira Roos, Carina Martins de Moraes, Roberta Sales Sousa, Roseli do Socorro Dias Monteiro   +9 more
doaj   +1 more source

Detection of alpha-thalassemias by multiplex polymerase chain reaction

Clinical Chemistry, 1994
Abstract Although alpha-thalassemia is the most common genetic abnormality in the world, there is currently no routine laboratory method to definitively identify individuals who are affected. We describe a rapid and simple method that utilizes deletion-sensitive primers to amplify normal DNA sequences.
L J, Bowie, P L, Reddy, M, Nagabhushan, P, Sevigny   +3 more
openaire   +2 more sources

Dataset for genotyping validation of cytochrome P450 2A6 whole-gene deletion (CYP2A6*4) by real-time polymerase chain reaction platforms

Data in Brief, 2015
This data article contains a supplementary figure and validation data relating to the research article entitled “Genotyping of wild-type cytochrome P450 2A6 and whole-gene deletion using human blood samples and a multiplex real-time polymerase chain ...
Makiko Shimizu, Tomoki Koyama, Izumi Kishimoto, Hiroshi Yamazaki   +3 more
doaj   +1 more source

Forensic validation studies of a novel 35-InDel multiplex polymerase chain reaction system

Journal of Forensic Science and Medicine, 2023
Background: A difficulty associated with forensic applications is the detection of degraded biological materials. Due to the large amplicon sizes of short tandem repeat alleles, valid genotyping results cannot be obtained from degraded biological ...
Tong Xie, Hui Xu, Congying Zhao, Yating Fang, Yongsong Zhou, Qiong Lan, Chunmei Shen, Bofeng Zhu   +7 more
doaj   +1 more source

Chemically modified primers for improved multiplex polymerase chain reaction [PDF]

Analytical Biochemistry, 2009
Multiplex polymerase chain reaction (PCR), the amplification of multiple targets in a single reaction, presents a new set of challenges that further complicate more traditional PCR setups. These complications include a greater probability for nonspecific amplicon formation and for imbalanced amplification of different targets, each of which can ...
Jonathan, Shum, Natasha, Paul
openaire   +2 more sources

Detection of bovine pestiviruses by a multiplex real-time polymerase chain reaction

Вопросы вирусологии, 2020
Introduction. Pestiviruses are the cause of reproductive problems, diseases of the gastrointestinal and respiratory tracts of animals. Three species are important for cattle: Pestivirus A, B, and H.
Aleksey V. Nefedchenko, Svetlana V. Koteneva, Tatyana I. Glotova, Alexander G. Glotov   +3 more
doaj   +1 more source