Results 21 to 30 of about 3,481,864 (291)

Chimerization of antibodies by isolation of rearranged genomic variable regions by the polymerase chain reaction [PDF]

, 1991
We describe a new method for amplification, by polymerase chain reaction (PCR), of rearranged segments encoding the variable part of light and heavy chains of an antibody (Ab) from the chromosomal DNA of hybridoma cells for the chimerization ofAbs.
Schwirzke, Marina, Kaluzab, Brigitte, Weissenhorn, Winfried, Weiss, Elisabeth H., Weidle, Ulrich H.   +4 more
core   +1 more source

Aetiological role of viral and bacterial infections in acute adult lower respiratory tract infection (LRTI) in primary care [PDF]

, 2005
Background: Lower respiratory tract infections (LRTI) are a common reason for consulting general practitioners (GPs). In most cases the aetiology is unknown, yet most result in an antibiotic prescription.
McHugh, TD, Gillespie, S H, Creer, D.D., Wallace, P.G., McHugh, T.D., Gillespie, S.H., Creer, D D, Johnston, S.L., Johnston, AR, Wallace, P G, Johnston, A R, Dilworth, J P, Sanderson, G, Sanderson, G., Johnston, S L, Ling, C., Johnston, A.R., McHugh, T D, Creer, DD, Dilworth, JP, Patel, S, Dilworth, J.P., Gillespie, SH, Ling, C, Patel, S., Wallace, PG, Johnston, SL   +26 more
core   +1 more source

A search for Clostridium difficile ribotypes 027 and 078 in Brazil

Brazilian Journal of Infectious Diseases, 2014
Toxigenic strains of Clostridium difficile may be disseminating. Here we prospectively screened patients with nosocomial diarrhoea in two hospitals in Brazil. To identify C.
Alexandre de Almeida Monteiro, Renata Neto Pires, Søren Persson, Edison Moraes Rodrigues Filho, Alessandro Comarú Pasqualotto   +4 more
doaj   +1 more source

Multiplex PCR-based titration (MPBT) assay for determination of infectious titers of the three Sabin strains of live poliovirus vaccine

Virology Journal, 2019
Background Conventional assays to titrate polioviruses usually test serial dilutions inoculated into replicate cell cultures to determine a 50% cytopathic endpoint, a process that is both time-consuming and laborious.
Hasmik Manukyan, Elvira Rodionova, Tatiana Zagorodnyaya, Tsai-Lien Lin, Konstantin Chumakov, Majid Laassri   +5 more
doaj   +1 more source

Specific detection of Salmonella spp. by multiplex polymerase chain reaction [PDF]

Applied and Environmental Microbiology, 1993
Three sets of oligonucleotide primers were used in the polymerase chain reaction (PCR) assay to detect Salmonella species. phoP primers specific to the phoP/phoQ loci of coliform pathogenic bacteria such as Salmonella, Shigella, Escherichia coli, and Citrobacter species served as presumptive indicators of enteric bacteria.
J S, Way, K L, Josephson, S D, Pillai, M, Abbaszadegan, C P, Gerba, I L, Pepper   +5 more
openaire   +2 more sources

Numerical and experimental study of a droplet-based PCR chip [PDF]

, 2007
A two-temperature continuous-flow polymerase chain reaction (PCR) polymer chip has been constructed that takes advantage of droplet technology to avoid sample contamination and adsorption at the surface.
MacAskill, Alexandra, Goddard, N.J., Emerson, David, Fielden, P.R., Zhang, Yonghao, Barber, Robert W., Mohr, S., Day, P.J.R.   +7 more
core   +4 more sources

OCCURRENCE OF CAMPYLOBACTER SPECIES IN CHICKENS BY MULTIPLEX POLYMERASE CHAIN REACTION [PDF]

Assiut Veterinary Medical Journal, 2017
The genus Campylobacter is one of great importance to public health because it includes several species that may cause diarrhea. Poultry and poultry products are known as important sources of human campylobacteriosis. 225 samples were collected from (75)
ASMAA GAHLAN YOUSEEF, A.I. IBRAHIM, AMAL SAYED, MONA SOBHY   +3 more
doaj   +1 more source

Detection of blaOXA-10 and blaOXA-48 Genes in Pseudomonas aeruginosa Clinical Isolates by Multiplex PCR

Journal of Medical Microbiology and Infectious Diseases, 2021
Introduction: The rapidly increasing extended-spectrum β-lactamase-producing Pseudomonas aeruginosa is a threat to health. This study aims to detect the rpoD gene and blaOXA-10 and blaOXA-48 genes in imipenem-resistant P.
Aghdas Bibi Hashemi, Mahboobeh Nakhaei Moghaddam, Mohammad Mahdi Forghanifard, Ehsan Yousefi   +3 more
doaj   +1 more source

Outcomes and clinical relevance of stool multiplex bacterial polymerase chain reaction in patients with acute diarrhea: single center experience [PDF]

The Korean Journal of Internal Medicine, 2020
Background/Aims Diagnostic stool multiplex polymerase chain reaction (PCR) testing has attracted considerable interest, because of its high sensitivity, short turnaround time, and ability to detect multiple organisms simultaneously.
Won Gun Kwack, Yun Jeong Lim, Ki Hwan Kwon, Jae Woo Chung, Jin Young Oh   +4 more
doaj   +1 more source

Detection of alpha-thalassemias by multiplex polymerase chain reaction

Clinical Chemistry, 1994
Abstract Although alpha-thalassemia is the most common genetic abnormality in the world, there is currently no routine laboratory method to definitively identify individuals who are affected. We describe a rapid and simple method that utilizes deletion-sensitive primers to amplify normal DNA sequences.
L J, Bowie, P L, Reddy, M, Nagabhushan, P, Sevigny   +3 more
openaire   +2 more sources