Results 21 to 30 of about 8,966 (157)

Comparison and transfer testing of multiplex ligation detection methods for GM plants [PDF]

, 2012
Background With the increasing number of GMOs on the global market the maintenance of European GMO regulations is becoming more complex. For the analysis of a single food or feed sample it is necessary to assess the sample for the presence of many GMO ...
van Dijk Jeroen P, van Hoef, A.M.A., Beenen Henriek G, van Dijk, Jeroen P., Voorhuijzen, Marleen, Marleen M Voorhuijzen, Theo W Prins, Hoef, A.M.A., van, Gruden, K., Jeroen P van Dijk, Kristina Gruden, Prins Theo W, Gabriella Ujhelyi, Voorhuijzen, M.M., Kok Esther J, Ujhelyi, Gabriella, Gruden Kristina, Morisset, D., Gruden, Kristina, AM Angeline Van Hoef, Morisset, Dany, van Dijk, J.P., Kok, E.J., Prins, Theo W., Esther J Kok, Henriek G Beenen, Beenen, H.G., Van Hoef, Angeline, Morisset Dany, Ujhelyi, G., Dijk, J.P., van, Dany Morisset, Angeline Van Hoef AM, Prins, T.W., Ujhelyi Gabriella, Kok, Esther, Voorhuijzen Marleen M, Beenen, Henriek G.   +37 more
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Applied genomics: data mining reveals species-specific malaria diagnostic targets more sensitive than 18S rRNA. [PDF]

, 2011
Accurate and rapid diagnosis of malaria infections is crucial for implementing species-appropriate treatment and saving lives. Molecular diagnostic tools are the most accurate and sensitive method of detecting Plasmodium, differentiating between ...
Jenna Oberstaller, Ganesh Srinivasamoorthy, Venkatachalam Udhayakumar, John W. Barnwell, Leopoldo Villegas, S. Patrick Kachur, Demas, Allison, Kissinger, Jessica C, Villegas, Leopoldo, Abdunoor M. Kabanywanyi, Jessica C. Kissinger, Deborah Sumari, Escalante, Ananias A, Lucchi, Naomi W, Udhayakumar, Venkatachalam, Sumari, Deborah, Peterson, David S, Ananias A. Escalante, Naomi W. Lucchi, Jeremy DeBarry, DeBarry, Jeremy, David S. Peterson, Oberstaller, Jenna, Allison Demas, Srinivasamoorthy, Ganesh, Kachur, S Patrick, Kabanywanyi, Abdunoor M, Barnwell, John W   +27 more
core   +1 more source

Detection of Microsatellite Instability by Fluorescence Multiplex Polymerase Chain Reaction [PDF]

The Journal of Molecular Diagnostics, 2000
We have created a clinical molecular diagnostic assay to test for microsatellite instability (MSI) at multiple loci simultaneously in paraffin-embedded surgical pathology colon resection specimens. This fluorescent multiplex polymerase chain reaction (PCR) assay analyzes the five primary microsatellite loci recommended at the 1997 National Cancer ...
K D, Berg, C L, Glaser, R E, Thompson, S R, Hamilton, C A, Griffin, J R, Eshleman   +5 more
openaire   +2 more sources

MCMC-ODPR : primer design optimization using Markov Chain Monte Carlo sampling [PDF]

, 2012
Background Next generation sequencing technologies often require numerous primer designs that require good target coverage that can be financially costly.
Kitchen, James, Kitchen James L, Allaby Robin G, Moore, Jonathan D., Palmer Sarah A, Allaby, Robin G., Moore Jonathan D, Palmer, Sarah A.   +7 more
core   +1 more source

Specific detection of Salmonella spp. by multiplex polymerase chain reaction [PDF]

Applied and Environmental Microbiology, 1993
Three sets of oligonucleotide primers were used in the polymerase chain reaction (PCR) assay to detect Salmonella species. phoP primers specific to the phoP/phoQ loci of coliform pathogenic bacteria such as Salmonella, Shigella, Escherichia coli, and Citrobacter species served as presumptive indicators of enteric bacteria.
J S, Way, K L, Josephson, S D, Pillai, M, Abbaszadegan, C P, Gerba, I L, Pepper   +5 more
openaire   +2 more sources

OCCURRENCE OF CAMPYLOBACTER SPECIES IN CHICKENS BY MULTIPLEX POLYMERASE CHAIN REACTION [PDF]

Assiut Veterinary Medical Journal, 2017
The genus Campylobacter is one of great importance to public health because it includes several species that may cause diarrhea. Poultry and poultry products are known as important sources of human campylobacteriosis. 225 samples were collected from (75)
ASMAA GAHLAN YOUSEEF, A.I. IBRAHIM, AMAL SAYED, MONA SOBHY   +3 more
doaj   +1 more source

Detection of blaOXA-10 and blaOXA-48 Genes in Pseudomonas aeruginosa Clinical Isolates by Multiplex PCR

Journal of Medical Microbiology and Infectious Diseases, 2021
Introduction: The rapidly increasing extended-spectrum β-lactamase-producing Pseudomonas aeruginosa is a threat to health. This study aims to detect the rpoD gene and blaOXA-10 and blaOXA-48 genes in imipenem-resistant P.
Aghdas Bibi Hashemi, Mahboobeh Nakhaei Moghaddam, Mohammad Mahdi Forghanifard, Ehsan Yousefi   +3 more
doaj   +1 more source

Outcomes and clinical relevance of stool multiplex bacterial polymerase chain reaction in patients with acute diarrhea: single center experience [PDF]

The Korean Journal of Internal Medicine, 2020
Background/Aims Diagnostic stool multiplex polymerase chain reaction (PCR) testing has attracted considerable interest, because of its high sensitivity, short turnaround time, and ability to detect multiple organisms simultaneously.
Won Gun Kwack, Yun Jeong Lim, Ki Hwan Kwon, Jae Woo Chung, Jin Young Oh   +4 more
doaj   +1 more source

Detection of alpha-thalassemias by multiplex polymerase chain reaction

Clinical Chemistry, 1994
Abstract Although alpha-thalassemia is the most common genetic abnormality in the world, there is currently no routine laboratory method to definitively identify individuals who are affected. We describe a rapid and simple method that utilizes deletion-sensitive primers to amplify normal DNA sequences.
L J, Bowie, P L, Reddy, M, Nagabhushan, P, Sevigny   +3 more
openaire   +2 more sources

Brazilian ground beef authentication by multiplex polymerase chain reaction

Ciência Rural, 2018
: The aim of the present study was to assess the efficacy ofmultiplex PCR in detecting the adulterationof commercially available ground beefvia addition and/orsubstitution ofground buffalo meat. Experimentally adulterated ground beefsamples were prepared
Andrey Carlos do Sacramento de Oliveira, Silvia Cristina da Silva Pedroso, Diogo José Cardilli, Fábio Pereira Leivas Leite, Gabrielle Virgínia Lopes Ferreira, Andréia Silva da Silva, Talita Bandeira Roos, Carina Martins de Moraes, Roberta Sales Sousa, Roseli do Socorro Dias Monteiro   +9 more
doaj   +1 more source