Brazilian ground beef authentication by multiplex polymerase chain reaction
: The aim of the present study was to assess the efficacy ofmultiplex PCR in detecting the adulterationof commercially available ground beefvia addition and/orsubstitution ofground buffalo meat. Experimentally adulterated ground beefsamples were prepared
Andrey Carlos do Sacramento de Oliveira +9 more
doaj +1 more source
Comparison and transfer testing of multiplex ligation detection methods for GM plants [PDF]
Background With the increasing number of GMOs on the global market the maintenance of European GMO regulations is becoming more complex. For the analysis of a single food or feed sample it is necessary to assess the sample for the presence of many GMO ...
van Dijk Jeroen P +37 more
core +1 more source
Detection of cholera toxin (ctxAand ctxAB) genes in Vibrio choleraeisolated from clinical and environmental samples in Limbang Sarawak by multiplex polymerase chain reaction (PCR) [PDF]
Aims:Cholera epidemics have been occurred in Malaysia since 1991 till 2003 which can be proved from the records by the Infectious Diseases Division of the Ministry of Health. Moreover, therewere also course of cholera epidemics from the year 1994 to 2003
Amirah Zakirah, Ja’afar +5 more
core +1 more source
Optimised padlock probe ligation and microarray detection of multiple (non-authorised) GMOs in a single reaction [PDF]
Background To maintain EU GMO regulations, producers of new GM crop varieties need to supply an event-specific method for the new variety. As a result methods are nowadays available for EU-authorised genetically modified organisms (GMOs), but only to a ...
Aarts, H.J.M. +17 more
core +1 more source
This data article contains a supplementary figure and validation data relating to the research article entitled “Genotyping of wild-type cytochrome P450 2A6 and whole-gene deletion using human blood samples and a multiplex real-time polymerase chain ...
Makiko Shimizu +3 more
doaj +1 more source
Detection of Microsatellite Instability by Fluorescence Multiplex Polymerase Chain Reaction [PDF]
We have created a clinical molecular diagnostic assay to test for microsatellite instability (MSI) at multiple loci simultaneously in paraffin-embedded surgical pathology colon resection specimens. This fluorescent multiplex polymerase chain reaction (PCR) assay analyzes the five primary microsatellite loci recommended at the 1997 National Cancer ...
K D, Berg +5 more
openaire +2 more sources
Applied genomics: data mining reveals species-specific malaria diagnostic targets more sensitive than 18S rRNA. [PDF]
Accurate and rapid diagnosis of malaria infections is crucial for implementing species-appropriate treatment and saving lives. Molecular diagnostic tools are the most accurate and sensitive method of detecting Plasmodium, differentiating between ...
Jenna Oberstaller +27 more
core +1 more source
Usefulness of Stool Multiplex Polymerase Chain Reaction Assays in Patients with Acute Diarrhea
Background/Aims: There is a recent increase in the use of stool multiplex PCR assay-based diagnostic tests in patients with acute diarrhea. We used multiplex PCR assays to analyze the distribution of diarrhea-causing bacteria and viruses, as well as the ...
Seo Hyun Kim +6 more
doaj +1 more source
Chemically modified primers for improved multiplex polymerase chain reaction [PDF]
Multiplex polymerase chain reaction (PCR), the amplification of multiple targets in a single reaction, presents a new set of challenges that further complicate more traditional PCR setups. These complications include a greater probability for nonspecific amplicon formation and for imbalanced amplification of different targets, each of which can ...
Jonathan, Shum, Natasha, Paul
openaire +2 more sources
Multiplex Manager 1.0: a cross-platform computer program that plans and optimizes multiplex PCR
Multiplex Manager 1.0 is a user-friendly cross-platform program that designs efficient combinations of existing genetic marker loci into multiplex polymerase chain reactions and optimizes using prior marker information. The program has the flexibility to
Clare E. Holleley, Paul G. Geerts
doaj +1 more source

