Results 31 to 40 of about 8,966 (157)

Diagnostic Accuracy of Multiplex Polymerase Chain Reaction in Early Onset Neonatal Sepsis

Children, 2023
Early onset neonatal sepsis is a significant contributor to neonatal morbidity and mortality. Although blood cultures remain the diagnostic gold standard, they detect pathogens in only a minority of suspected cases.
Anja Stein, Daniel Soukup, Peter-Michael Rath, Ursula Felderhoff-Müser   +3 more
doaj   +1 more source

Chemically modified primers for improved multiplex polymerase chain reaction [PDF]

Analytical Biochemistry, 2009
Multiplex polymerase chain reaction (PCR), the amplification of multiple targets in a single reaction, presents a new set of challenges that further complicate more traditional PCR setups. These complications include a greater probability for nonspecific amplicon formation and for imbalanced amplification of different targets, each of which can ...
Jonathan, Shum, Natasha, Paul
openaire   +2 more sources

Dataset for genotyping validation of cytochrome P450 2A6 whole-gene deletion (CYP2A6*4) by real-time polymerase chain reaction platforms

Data in Brief, 2015
This data article contains a supplementary figure and validation data relating to the research article entitled “Genotyping of wild-type cytochrome P450 2A6 and whole-gene deletion using human blood samples and a multiplex real-time polymerase chain ...
Makiko Shimizu, Tomoki Koyama, Izumi Kishimoto, Hiroshi Yamazaki   +3 more
doaj   +1 more source

A molecular approach (multiplex polymerase chain reaction) for diagnosis of rhinosporidiosis [PDF]

Indian Journal of Otolaryngology and Head and Neck Surgery, 2002
A New multiplex PCR have been developed in our laboratory using primer sets, aiming amplification of both D.N.A target fragments obtained in 18S RNA of commonly encountered fungi in human being and in Rhinosporidium seeberi using F1-fw/F2-rev (500 bp target) and Rhino-fw/ Rhino-rev (.177 bp target).
Somnath, Saha, Dibyakanti, Mondal, Dimple, Khetawat, Atanu, Roy, Sekhar, Chakrabarti, Basudev, Bhattacharya   +5 more
openaire   +2 more sources

Multiplex bacterial polymerase chain reaction in a cohort of patients with pleural effusion [PDF]

BMC Infectious Diseases, 2020
Abstract Background The identification of the pathogens in pleural effusion has mainly relied on conventional bacterial culture or single species polymerase chain reaction (PCR), both with relatively low sensitivity. We investigated the efficacy of a commercially available multiplex bacterial PCR assay developed for pneumonia to identify the pathogens ...
Franchetti L, Schumann DM, Tamm M, Jahn K, Stolz D   +4 more
openaire   +4 more sources

Aetiological role of viral and bacterial infections in acute adult lower respiratory tract infection (LRTI) in primary care

, 2005
Background: Lower respiratory tract infections (LRTI) are a common reason for consulting general practitioners (GPs). In most cases the aetiology is unknown, yet most result in an antibiotic prescription.
McHugh, TD, Gillespie, S H, Creer, D.D., Wallace, P.G., McHugh, T.D., Gillespie, S.H., Creer, D D, Johnston, S.L., Johnston, AR, Wallace, P G, Johnston, A R, Dilworth, J P, Sanderson, G, Sanderson, G., Johnston, S L, Ling, C., Johnston, A.R., McHugh, T D, Creer, DD, Dilworth, JP, Patel, S, Dilworth, J.P., Gillespie, SH, Ling, C, Patel, S., Wallace, PG, Johnston, SL   +26 more
core   +1 more source

Forensic validation studies of a novel 35-InDel multiplex polymerase chain reaction system

Journal of Forensic Science and Medicine, 2023
Background: A difficulty associated with forensic applications is the detection of degraded biological materials. Due to the large amplicon sizes of short tandem repeat alleles, valid genotyping results cannot be obtained from degraded biological ...
Tong Xie, Hui Xu, Congying Zhao, Yating Fang, Yongsong Zhou, Qiong Lan, Chunmei Shen, Bofeng Zhu   +7 more
doaj   +1 more source

Plasmodium diversity in non-malaria individuals from the Bioko Island in Equatorial Guinea (West Central-Africa). [PDF]

, 2006
BACKGROUND: In this paper we analyse the Plasmodium sp. prevalence in three villages with different isolation status on the island of Bioko (Equatorial Guinea) where malaria is a hyper-endemic disease.
Auñón, Antonio Sarrión, Ochando, Jordi, Agustín LLanes, Sarrión Auñón, Antonio, José M Rubio, Ortega Jorge, Royo, Jesús Roche, Royo Jesús, Roche, Jesus, Jesús Royo, Guerra-Neira Ana, Auñón Antonio, LLanes Agustín, Berzosa, Pedro, Diaz Pedro, Ortega, Jorge Cano, Jorge Ortega, Ana Guerra-Neira, Diaz, Pedro Berzosa, Pedro Diaz, Rubio Muñoz, Jose Miguel, Benito, Agustin, Antonio Auñón, Llanes, Agustín Benito, Rubio, José M, Guerra-Neira, Ana, Rubio José M   +26 more
core   +1 more source

Multiplex Manager 1.0: a cross-platform computer program that plans and optimizes multiplex PCR

BioTechniques, 2009
Multiplex Manager 1.0 is a user-friendly cross-platform program that designs efficient combinations of existing genetic marker loci into multiplex polymerase chain reactions and optimizes using prior marker information. The program has the flexibility to
Clare E. Holleley, Paul G. Geerts
doaj   +1 more source

Usefulness of Stool Multiplex Polymerase Chain Reaction Assays in Patients with Acute Diarrhea

The Korean Journal of Gastroenterology, 2022
Background/Aims: There is a recent increase in the use of stool multiplex PCR assay-based diagnostic tests in patients with acute diarrhea. We used multiplex PCR assays to analyze the distribution of diarrhea-causing bacteria and viruses, as well as the ...
Seo Hyun Kim, You Sun Kim, Seung Hyuk Kim, Won Eui Yoon, Hee Jun Myung, Jeong Seop Moon, Dong Hee Whang   +6 more
doaj   +1 more source